| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,548,960 | C→T | 13.5% | L13L (CTG→TTG) | kbl → | glycine C‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,548,960 | 0 | C | T | 13.5% | 165.7 / 15.6 | 59 | L13L (CTG→TTG) | kbl | glycine C‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (30/21); new base T (5/3); total (35/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
ATGTGCTGGAATTTGCCCTGTCTGGAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTACGTCTGCGCAGCAAGCAGATAT > minE/2548890‑2549030 | atGTGCTGGAATTTGCCCTGTCTGGAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATc > 1:2704255/1‑71 (MQ=255)atGTGCTGGAATTTGCCCTGTCTGGAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAAAGATc > 1:2655210/1‑71 (MQ=255) tgtgCTGGAATTTGCCCTGTCTGGAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCt > 1:2971222/1‑71 (MQ=255) tgtgCTGGAATTTGCCCTGTCTGGAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCt > 1:1950573/1‑71 (MQ=255) tgtgCTGGAATTTGCCCTGTCTGGAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCt > 1:3254434/1‑71 (MQ=255) tgtgCTGGAATTTGCCCTGTCTGGAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCt > 1:139842/1‑71 (MQ=255) tgtgCTGGAATTTGCCCTGTCTGGAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCt > 1:2772000/1‑71 (MQ=255) gCTGGAATTTGCCCTGTCTGGAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTgg > 1:2383972/1‑70 (MQ=255) cTGGAATTTGCCCTGTCTGGAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGaa < 1:1939735/71‑1 (MQ=255) cTGGAATTTGCCCTGTCTGGAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGa < 1:2406042/70‑1 (MQ=255) ggAATTTGCCCTGTCTGGAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTg < 1:1507518/66‑1 (MQ=255) aaTTTGCCCTGTCTGGAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATTTGGAAACCg < 1:1773210/71‑1 (MQ=255) tGCCCTGACTGGAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACg > 1:3196345/1‑71 (MQ=255) tCTGGAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCg < 1:2212314/60‑1 (MQ=255) tCTGGAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGa < 1:2052152/70‑1 (MQ=255) cTGGAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATTTGGa < 1:2494095/54‑1 (MQ=255) ggAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAggg > 1:932277/1‑71 (MQ=255) ggAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAgg < 1:2177934/70‑1 (MQ=255) ggAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAgg < 1:16831/70‑1 (MQ=255) gagaATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCg < 1:2771855/56‑1 (MQ=255) gagaATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCg < 1:2704877/56‑1 (MQ=255) gagaATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAggg < 1:2042279/70‑1 (MQ=255) gagaATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAGGgt > 1:1643106/1‑71 (MQ=255) aaTCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGaa > 1:1739854/1‑64 (MQ=255) aaTCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGaa > 1:903950/1‑64 (MQ=255) tCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAGGgttgtt > 1:1724850/1‑70 (MQ=255) tCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAGGgttgtt > 1:68207/1‑70 (MQ=255) aTGCGTGGAGAATTTTATCAGCAGTTAACCAACGATTTGGAAACCGCACGGGCGGAAGGGTTGTTTAaag > 1:2859208/1‑70 (MQ=255) tGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAggg > 1:2091979/1‑59 (MQ=255) tGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAggg > 1:1332456/1‑59 (MQ=255) tGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAaagaa < 1:3354069/71‑1 (MQ=255) tGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAaagaa < 1:782329/71‑1 (MQ=255) tGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAaag < 1:2761743/69‑1 (MQ=255) tGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAaag < 1:97701/69‑1 (MQ=255) gCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAaag > 1:1637622/1‑68 (MQ=255) tGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAgc > 1:231704/1‑71 (MQ=255) gagaATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAgcg > 1:2824108/1‑70 (MQ=255) agaATTTTATCAGCAGTTAACCAACGGTCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCa < 1:2527410/71‑1 (MQ=255) agaATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAgcg < 1:2567520/69‑1 (MQ=255) ttttATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCat > 1:3363786/1‑68 (MQ=255) aTCAGCAGTTAACCAACGATTTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTAc < 1:1131720/70‑1 (MQ=255) tCAGCAGTTAACCAACGATTTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCat > 1:2712192/1‑63 (MQ=255) tCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAgg < 1:1208119/42‑1 (MQ=255) cagcagTTAACCAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAaag < 1:1363495/52‑1 (MQ=255) tAACCAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTACGTCTGCgcag > 1:2428734/1‑71 (MQ=255) aaCCAACGATTTGGAAACCGCACGGGCGGAAGGGTTGTTTaaa > 1:1731199/1‑43 (MQ=255) aaCCAACGATTTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTACGt > 1:252189/1‑62 (MQ=255) aaCCAACGATTTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTACGt > 1:2208913/1‑62 (MQ=255) aaCCAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTACGTCTGCgcag > 1:1637464/1‑70 (MQ=255) aaCCAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTACGTCTGCgcag > 1:2440006/1‑70 (MQ=255) aCCAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTACGTCTGCgcagca < 1:2915963/71‑1 (MQ=255) cAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTACGt > 1:559091/1‑59 (MQ=255) cAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTACGt > 1:611724/1‑59 (MQ=255) cAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTACGt > 1:1158281/1‑59 (MQ=255) cAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTACGTCTGCgcagca < 1:545997/69‑1 (MQ=255) aaCGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTTCGTCTGCGCagcaag > 1:1197358/1‑70 (MQ=255) aaCGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTACGTCTGCGCagcaag > 1:3336372/1‑70 (MQ=255) aaCGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTACGTCTGCGCagcaag > 1:411093/1‑70 (MQ=255) aCGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTACGTCTGCGCagcaagca < 1:1134320/71‑1 (MQ=255) aCGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTACGTCTGCGCagcaag > 1:339436/1‑69 (MQ=255) aCGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTACGTCTGCGCagcaag > 1:1113140/1‑69 (MQ=255) cTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTACGTCTGCGCAGCAAGCAGatat > 1:2168744/1‑71 (MQ=255) | ATGTGCTGGAATTTGCCCTGTCTGGAGAATCGCAATGCGTGGAGAATTTTATCAGCAGTTAACCAACGATCTGGAAACCGCACGGGCGGAAGGGTTGTTTAAAGAAGAGCGCATTATTACGTCTGCGCAGCAAGCAGATAT > minE/2548890‑2549030 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |