| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,776,458 | A→G | 9.8% | Y410H (TAT→CAT) | arpA ← | regulator of acetyl CoA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,776,458 | 0 | A | G | 9.8% | 170.6 / 10.2 | 61 | Y410H (TAT→CAT) | arpA | regulator of acetyl CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (25/30); new base G (3/3); total (28/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CAGAAAAACCATTTTTATCTTTTGCCTCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGCCAAATA > minE/2776389‑2776527 | cAGAAAAACCATTTTTATCTTTTGCCTCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCatat < 1:2915484/71‑1 (MQ=255) aGAAAAACCATTTTTATCTTTTGCCTCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATc < 1:954080/71‑1 (MQ=255) aGAAAAACCATTTTTATCTTTTGCCTCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATc < 1:1778629/71‑1 (MQ=255) aGAAAAACCATTTTTATCTTTTGCCTCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATc < 1:3216379/71‑1 (MQ=255) gAAAAACCATTTTTATCTTTTGCCTCCAGAATACGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATcc < 1:1325821/71‑1 (MQ=255) cATTTTTATCTTTTGCCTCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCt < 1:3158400/71‑1 (MQ=255) cATTTTTATCTTTTGCCTCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCt < 1:2939884/71‑1 (MQ=255) aTTTTTATCTTTTGCCTCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCt > 1:2343815/1‑70 (MQ=255) aTTTTTATCTTTTGCCTCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCt > 1:2752133/1‑70 (MQ=255) aTTTTTATCTTTTGCCTCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTg < 1:1683292/71‑1 (MQ=255) tttATCTTTTGCCTCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGAc < 1:273176/70‑1 (MQ=255) aTCTTTTGCCTCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAAt > 1:2753545/1‑70 (MQ=255) tCTTTTGCCTCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATg < 1:2787268/70‑1 (MQ=255) ttttGCCTCCAGAATATGCATGAGATTTTTTTTCAAGAGTAATCCTTCATATCCTGTTTCTGACAATGAAt > 1:1820162/1‑71 (MQ=255) ttGCCTCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGa < 1:1634919/67‑1 (MQ=255) ttGCCTCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGa < 1:304186/67‑1 (MQ=255) ccTCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGaa < 1:2074221/70‑1 (MQ=255) cTCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGaaaa < 1:494387/71‑1 (MQ=255) tCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGt < 1:2387397/50‑1 (MQ=255) tCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCt < 1:1501633/54‑1 (MQ=255) tCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGa < 1:1134987/67‑1 (MQ=255) ccAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGaaaa < 1:2041092/69‑1 (MQ=255) aGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTg < 1:1298871/52‑1 (MQ=255) aGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGAAAATa > 1:2880293/1‑69 (MQ=255) gAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGa < 1:108573/63‑1 (MQ=255) aaTATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATGTc < 1:192364/41‑1 (MQ=255) aaTATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATGTc < 1:308458/41‑1 (MQ=255) aaTATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATGTc < 1:2327027/41‑1 (MQ=255) tatGCATGAGATTTTTTTTCGAGAGTAATCCTTCATGTCCt > 1:1277888/1‑41 (MQ=255) tatGCATGAGATTTTTTTTCGAGAGTAATCCTTCATGTCCt > 1:1777589/1‑41 (MQ=255) tatGCATGAGATTTTTTTTCGAGAGTAATCCTTCATGTCCt > 1:1552078/1‑41 (MQ=255) tatGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCa < 1:296540/71‑1 (MQ=255) tGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGtt > 1:16576/1‑42 (MQ=255) tGAGATTTTTTTTCGAGAGTAATCCTTCATATCCt < 1:2007593/35‑1 (MQ=255) tGAGATTTTTTTTCGAGAGTAATCCTTCATATCCt < 1:2043692/35‑1 (MQ=255) tGAGATTTTTTTTCGAGAGTAATCCTTCATATCCt < 1:279954/35‑1 (MQ=255) tGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGa < 1:1908813/54‑1 (MQ=255) tGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGata < 1:3182976/71‑1 (MQ=255) agaTTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATc > 1:3091958/1‑71 (MQ=255) agaTTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATc > 1:3028894/1‑71 (MQ=255) gaTTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATcc > 1:401410/1‑71 (MQ=255) gaTTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATcc > 1:2880246/1‑71 (MQ=255) aTTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCg > 1:953057/1‑71 (MQ=255) ttttttttCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGc < 1:2380177/71‑1 (MQ=255) tttttttCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGaaaa > 1:3316219/1‑51 (MQ=255) tttttttCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGatat > 1:2507993/1‑66 (MQ=255) tttttttCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGc > 1:712287/1‑70 (MQ=255) tttttttCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCa > 1:1098866/1‑71 (MQ=255) tttttCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCAt < 1:928967/70‑1 (MQ=255) agTAATCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAAtt < 1:2069110/71‑1 (MQ=255) agTAATCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAAt > 1:1504253/1‑70 (MQ=255) tAATCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTta < 1:2675203/71‑1 (MQ=255) aTCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTta > 1:2845919/1‑69 (MQ=255) aTCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTta > 1:1524194/1‑69 (MQ=255) tCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAg > 1:1900039/1‑71 (MQ=255) cTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAAc < 1:565857/43‑1 (MQ=255) cTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGa > 1:2002387/1‑45 (MQ=255) cTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGc > 1:137986/1‑70 (MQ=255) cTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGc > 1:2175161/1‑70 (MQ=255) tCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCAt > 1:1435312/1‑52 (MQ=255) tCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAga > 1:402731/1‑67 (MQ=255) cATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCAt > 1:3236600/1‑51 (MQ=255) atatCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGCCaaa < 1:1661006/70‑1 (MQ=255) atatCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGCCaaa < 1:613269/70‑1 (MQ=255) atatCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGCCaaa < 1:2874635/70‑1 (MQ=255) tatCCTGTTTCTGACAATGAATTGAAAATAGTCTCAAc < 1:2155390/38‑1 (MQ=255) tatCCTGTTTCTGACAATGAATTGAAAATAGTCTCAAc < 1:2518012/38‑1 (MQ=255) tatCCTGTTTCTGACAATGAATTGAAAATAGTCTCAAc < 1:2573526/38‑1 (MQ=255) tatCCTGTTTCTGACAATGAATTGAAAATAGTCTCAAc < 1:1134726/38‑1 (MQ=255) tatCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCa < 1:950493/48‑1 (MQ=255) tatCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGCCAAata < 1:900584/71‑1 (MQ=255) | CAGAAAAACCATTTTTATCTTTTGCCTCCAGAATATGCATGAGATTTTTTTTCGAGAGTAATCCTTCATATCCTGTTTCTGACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGCCAAATA > minE/2776389‑2776527 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |