| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 357,299 | T→C | 16.4% | F5L (TTT→CTT) | sfmA → | predicted fimbrial‑like adhesin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 357,299 | 0 | T | C | 16.4% | 179.3 / 28.6 | 73 | F5L (TTT→CTT) | sfmA | predicted fimbrial‑like adhesin protein |
| Reads supporting (aligned to +/- strand): ref base T (31/30); new base C (5/7); total (36/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
AAAGTAATCGGTTATATACGGATGTGGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATG > minE/357231‑357365 | aaaGTAATCGGTTATATACGGATGTGGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGatt > 1:33361/1‑70 (MQ=255) aGTAATCGGTTATATACGGATGTGGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGatttat < 1:2892439/71‑1 (MQ=255) cGGTTATATACGGATGTGGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTc > 1:2706860/1‑71 (MQ=255) cGGTTATATACGGATGTGGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTc > 1:1568075/1‑71 (MQ=255) gTTATATACGGATGTGGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTg > 1:1179309/1‑71 (MQ=255) ttATATACGGATGTGGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTgc < 1:2695004/71‑1 (MQ=255) ttATATACGGATGTGGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTgc < 1:997192/71‑1 (MQ=255) ttATATACGGATGTGGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTgc < 1:1542555/71‑1 (MQ=255) tataCGGATGTGGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGat < 1:1957937/55‑1 (MQ=255) tataCGGATGTGGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCgct < 1:1033397/71‑1 (MQ=255) tataCGGATGTGGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCgct < 1:1284589/71‑1 (MQ=255) aCGGATGTGGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGatt < 1:502996/53‑1 (MQ=255) aCGGATGTGGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGatt < 1:1361049/53‑1 (MQ=255) aCGGATGTGGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGatt < 1:2209614/53‑1 (MQ=255) cGGATGTGGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCgctggct > 1:1467821/1‑71 (MQ=255) cGGATGTGGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGACTTATTTCGTCTGCgctggct > 1:1071774/1‑71 (MQ=255) tgGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGACTTATTTCGTCTGCGCTGGCTGCCGc < 1:1978191/70‑1 (MQ=255) tgGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGACTTATTTCGTCTGCGCTGGCTGCCGc < 1:1971107/70‑1 (MQ=255) gAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCAc < 1:2747028/70‑1 (MQ=255) aGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTa < 1:122978/71‑1 (MQ=255) aGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTa < 1:894979/71‑1 (MQ=255) aGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTa < 1:1494916/71‑1 (MQ=255) aGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTa < 1:1181402/71‑1 (MQ=255) tCGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCgctg < 1:79753/57‑1 (MQ=255) tCGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTa > 1:2141905/1‑69 (MQ=255) tCGATAAATGAGATTGAAGGAATACATATGAAATTAAGATTTATTTCGTCTGCgctg < 1:869746/57‑1 (MQ=255) cGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCt > 1:2081392/1‑50 (MQ=255) gATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCgctggc > 1:3210833/1‑57 (MQ=255) gATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTc < 1:2847632/70‑1 (MQ=255) aaaTGAGATTGAAGGAATATATATGAAATTAAGACTTATTTCGTCTGCGCTGGCTGCCGCACTATTcgccg > 1:3068961/1‑71 (MQ=255) aaTGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTcgccgc < 1:2959634/71‑1 (MQ=255) aaTGAGATTGAAGGAATATATATGAAATTAAGACTTATTTCGTCTGCGCTGGCTGCCGCACTATTcgccgc < 1:129386/71‑1 (MQ=255) aaTGAGATTGAAGGAATATATATGAAATTAAGACTTATTTCGTCTGCGCTGGCTGCCGCACTATTcgccgc < 1:900621/71‑1 (MQ=255) aTGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTcgccgc > 1:3097874/1‑70 (MQ=255) aTGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTcgccgc > 1:78122/1‑70 (MQ=255) aTGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCAGCGCTGGCTGCCGCACTATTcgccg > 1:3048186/1‑69 (MQ=255) agaTTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCgctggc > 1:805751/1‑49 (MQ=255) gaTTGAAGGAATATATATGAAATTAAGACTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACgg > 1:416488/1‑71 (MQ=255) ttGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACggg > 1:972500/1‑70 (MQ=255) gAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCgctggc > 1:2101308/1‑44 (MQ=255) gAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTcgccg > 1:532332/1‑61 (MQ=255) gAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTa > 1:2285994/1‑64 (MQ=255) gAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTa > 1:2333659/1‑64 (MQ=255) gAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTa < 1:887176/70‑1 (MQ=255) gAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAg > 1:992410/1‑71 (MQ=255) aaGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTa < 1:348331/69‑1 (MQ=255) aaGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGt < 1:2558864/71‑1 (MQ=255) aGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACggg < 1:439716/66‑1 (MQ=255) aGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACggg < 1:435649/66‑1 (MQ=255) aGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGtt < 1:483656/71‑1 (MQ=255) aGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGtt < 1:2059406/71‑1 (MQ=255) ggAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTcgccg > 1:3164207/1‑58 (MQ=255) ggAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACggg > 1:1182695/1‑65 (MQ=255) ggAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACggg > 1:572919/1‑65 (MQ=255) gAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTAt > 1:2579184/1‑71 (MQ=255) gAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTAt > 1:1623424/1‑71 (MQ=255) aaTATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTAtt > 1:1337837/1‑51 (MQ=255) aaTATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTAtg < 1:496300/71‑1 (MQ=255) atatatatGAAATTAAGACTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTAtgc > 1:1950927/1‑71 (MQ=255) tatatatGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGt < 1:81526/65‑1 (MQ=255) tatatatGAAATTAAGACTTATTTCGTCTGCGCTGGCTGCCGCACTATTcgcc < 1:2850285/53‑1 (MQ=255) atatatGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGc < 1:3057525/42‑1 (MQ=255) atatatGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGc < 1:2682291/42‑1 (MQ=255) atatatGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGc < 1:2544209/42‑1 (MQ=255) tatatGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTAtgctgc > 1:1970658/1‑71 (MQ=255) atGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGt > 1:2392904/1‑71 (MQ=255) tGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTa < 1:1038598/57‑1 (MQ=255) tGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACCGGTAGTTATGCTGCCGtt > 1:3039752/1‑71 (MQ=255) tGAAATTAAGACTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGt < 1:1022605/70‑1 (MQ=255) gAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACg > 1:472008/1‑52 (MQ=255) aaaTTAAGATTTATTTCGTCTGCGCTGGCTGCCGCGCTATTCGCCGCTACGGGTAGTTATGCTGCCGTTg < 1:2956785/70‑1 (MQ=255) aaaTTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTg < 1:1036977/70‑1 (MQ=255) aaaTTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTg > 1:2076720/1‑70 (MQ=255) aaTTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTa < 1:3320451/71‑1 (MQ=255) aTTAAGACTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGATGCCGTTg > 1:1191361/1‑68 (MQ=255) ttAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTg > 1:3353369/1‑67 (MQ=255) ttAAGACTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTg < 1:1592032/67‑1 (MQ=255) aaGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAg > 1:298483/1‑68 (MQ=255) aaGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATg > 1:1801756/1‑71 (MQ=255) | AAAGTAATCGGTTATATACGGATGTGGAGTCGATAAATGAGATTGAAGGAATATATATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATG > minE/357231‑357365 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |