| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,005,755 | G→C | 7.1% | intergenic (‑161/‑363) | pntA ← / → ydgH | pyridine nucleotide transhydrogenase, alpha subunit/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,005,755 | 0 | G | C | 7.1% | 258.4 / 7.8 | 84 | intergenic (‑161/‑363) | pntA/ydgH | pyridine nucleotide transhydrogenase, alpha subunit/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (47/31); new base C (2/4); total (49/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
TAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAG > minE/1005685‑1005818 | tAAAAT‑AATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAg > 1:327077/1‑70 (MQ=255) aaaaTAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAg < 1:2953343/70‑1 (MQ=255) aaaaTAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAg < 1:772792/70‑1 (MQ=255) tAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGt < 1:1448057/62‑1 (MQ=255) aaCAAACGCCCTATAACGTACTGAAAATTATGCCTGTGAGCTAGCGCCAAAAATCAGTATTTCGGCGTg < 1:1767510/69‑1 (MQ=255) aCAAACGCCCTATAACTTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAAc < 1:2668686/71‑1 (MQ=255) cAAACGCCCTATAACGTACTGAAAATTATGTCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACt > 1:1052079/1‑71 (MQ=255) cAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACt > 1:2276066/1‑71 (MQ=255) aaCGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTa > 1:798552/1‑55 (MQ=255) aCGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGtt < 1:2519115/64‑2 (MQ=255) cGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCg > 1:471676/1‑69 (MQ=255) cGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGAGCCAAAAATCAGTATTTCGGCGTGAACTCGc > 1:1308692/1‑70 (MQ=255) ccTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTa > 1:1252668/1‑50 (MQ=255) ccTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCaaaa < 1:2851099/71‑1 (MQ=255) ccTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCaaaa < 1:254056/71‑1 (MQ=255) ccTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCaaaa < 1:899598/71‑1 (MQ=255) ccTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCaaaa < 1:1132493/71‑1 (MQ=255) tataACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAg < 1:2600662/46‑1 (MQ=255) tataACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAg < 1:3225206/46‑1 (MQ=255) tataACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAg < 1:58217/46‑1 (MQ=255) ataACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAata > 1:1616043/1‑70 (MQ=255) ataACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAAGCAGTATTTCGGCGTGAACTCGCAAAatat > 1:2958394/1‑71 (MQ=255) taACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCaaaa < 1:676598/67‑1 (MQ=255) taACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAatat > 1:663737/1‑70 (MQ=255) taACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAatat > 1:918048/1‑70 (MQ=255) taACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAatat > 1:1324093/1‑70 (MQ=255) taACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAata > 1:842081/1‑69 (MQ=255) taACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATAtt < 1:2225457/71‑1 (MQ=255) taACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCACTAt < 1:2128834/47‑1 (MQ=255) taACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCACTAt < 1:482110/47‑1 (MQ=255) taACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCACTAt < 1:2125061/47‑1 (MQ=255) taACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCACTAt < 1:2898071/47‑1 (MQ=255) taACGTACTGAAAATAATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAata > 1:2150000/1‑69 (MQ=255) aaCGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTa > 1:3152261/1‑45 (MQ=255) aaCGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTc > 1:1240581/1‑60 (MQ=255) aaCGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTc > 1:165816/1‑60 (MQ=255) aaCGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTc > 1:723943/1‑60 (MQ=255) aaCGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTc > 1:2205340/1‑60 (MQ=255) aCGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCaaaa > 1:128138/1‑65 (MQ=255) aCGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTaa > 1:1920525/1‑71 (MQ=255) aCGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTaa > 1:422457/1‑71 (MQ=255) aCGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTaa > 1:93476/1‑71 (MQ=255) aCGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTaa > 1:2028042/1‑71 (MQ=255) aCGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTaa > 1:2914229/1‑71 (MQ=255) aCGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCACTAtca > 1:119636/1‑45 (MQ=255) aCGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCACTAtca > 1:415643/1‑45 (MQ=255) cGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAAc < 1:1385220/56‑1 (MQ=255) cGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAAc < 1:900850/71‑1 (MQ=255) cGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAAc > 1:2655556/1‑71 (MQ=255) aCTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAg < 1:1115252/38‑1 (MQ=255) aCTGAAAATTATGCCTATGATCTAGCGCCAAAAATCAg < 1:2618322/38‑1 (MQ=255) cTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGt < 1:2598103/38‑1 (MQ=255) cTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCg < 1:3229112/55‑1 (MQ=255) tGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAg < 1:1417437/36‑1 (MQ=255) tGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAg < 1:2487071/36‑1 (MQ=255) tGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAg < 1:3120905/36‑1 (MQ=255) tGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTg < 1:1118219/48‑1 (MQ=255) tGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAAc < 1:306723/66‑1 (MQ=255) gAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTa > 1:3296434/1‑37 (MQ=255) gAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTAt > 1:3283218/1‑38 (MQ=255) gAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGaa > 1:3018393/1‑49 (MQ=255) gAAAATTATGCCTGTCATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGAtt > 1:42623/1‑69 (MQ=255) aaTTATGCCTGTGATCTAGCGCCAAAAATCAGTAtt > 1:1218017/1‑36 (MQ=255) aaTTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCg > 1:3349092/1‑50 (MQ=255) aaTTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCg > 1:1640068/1‑50 (MQ=255) aaTTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCg > 1:1661099/1‑50 (MQ=255) aaTTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGcc > 1:2602782/1‑71 (MQ=255) ttATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCg > 1:553323/1‑48 (MQ=255) ttATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGt < 1:2877703/71‑1 (MQ=255) ttATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGt < 1:2936196/71‑1 (MQ=255) ttATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAACATTAACGATTCAGCCGt < 1:793839/71‑1 (MQ=255) aTGCCTGTGATCTAGCGCCAAAAATCAGTGTTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGa > 1:1481146/1‑71 (MQ=255) tgtgATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTg > 1:2986122/1‑71 (MQ=255) tgATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGt > 1:2777130/1‑68 (MQ=255) gcgcCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATaa > 1:2957142/1‑68 (MQ=255) gcgcCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATa < 1:2532129/67‑1 (MQ=255) gcgcCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATa < 1:1460574/67‑1 (MQ=255) gcgcCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATa < 1:1396213/67‑1 (MQ=255) gcgcCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATa < 1:722975/67‑1 (MQ=255) gcgcCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATa < 1:172268/67‑1 (MQ=255) gcgcCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATa < 1:50158/67‑1 (MQ=255) gcgcCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATa < 1:2579504/67‑1 (MQ=255) gcgcCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAaca > 1:948219/1‑71 (MQ=255) cgcCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAacac < 1:2956776/71‑1 (MQ=255) cgcCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAacac < 1:409079/71‑1 (MQ=255) tccAAAAATCAGTATTTCGGCGTTAACTCGCAAAATATTAACGATTCAGCCGTg < 1:1960605/53‑1 (MQ=255) gcCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAAc > 1:239971/1‑42 (MQ=255) gcCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAAc > 1:297719/1‑42 (MQ=255) gcCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAAc > 1:2886271/1‑42 (MQ=255) aaaaaTCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCt > 1:2528633/1‑69 (MQ=255) aaaaaTCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCt > 1:1764813/1‑69 (MQ=255) aaaaaTCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCt > 1:1801827/1‑69 (MQ=255) aaaaaTCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCt > 1:2566339/1‑69 (MQ=255) aaaaTCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAg < 1:766664/71‑1 (MQ=255) aaaaTCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAg < 1:9605/71‑1 (MQ=255) | TAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAG > minE/1005685‑1005818 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |