| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,668,051 | G→A | 7.4% | Q378Q (CAG→CAA) | xseA → | exonuclease VII, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,668,051 | 0 | G | A | 7.4% | 218.1 / 5.8 | 67 | Q378Q (CAG→CAA) | xseA | exonuclease VII, large subunit |
| Reads supporting (aligned to +/- strand): ref base G (39/23); new base A (2/3); total (41/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
CGAAGATTCATCGCGCGCAAACGCGCATTCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCAACGC > minE/1667981‑1668121 | cGAAGATTCATCGCGCGCAAACGCGCATTCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAg < 1:1834460/71‑1 (MQ=255)cGAAGATTCATCGCGCGCAAACGCGCATTCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAg > 1:2776228/1‑71 (MQ=255) gATTCATCGCGCGCAAACGCGCATTCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTa < 1:1328964/71‑1 (MQ=255) tCATCGCGCGCAAACGCGCATTCAGCAATTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAg > 1:2827011/1‑69 (MQ=255) tCATCGCGCGCAAACGCGCATTCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAgcg > 1:2726229/1‑71 (MQ=255) tCATCGCGCGCAAACGCGCATTCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAgcg > 1:1599255/1‑71 (MQ=255) tCATCGCGCGCAAACGCGCATTCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAg > 1:2880410/1‑69 (MQ=255) cATCGCGCGCAAACGCGCATTCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAgcgc > 1:2538778/1‑71 (MQ=255) cATCGCGCGCAAACGCGCATTCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAgcgc > 1:3342998/1‑71 (MQ=255) aTCGCGCGCAAACGCGCATTCAGCAACTGCAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAgcgc < 1:1545338/70‑1 (MQ=255) cgcgcgCAAACGCGCATTCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCAc > 1:1217911/1‑71 (MQ=255) gcgcgcAAACGCGCATTCAGCAACTGGAATATGGTTTAGCAGAAACCCTGCGCGCACAACTTAGCGCCAc < 1:998507/70‑1 (MQ=255) gcgcgcAAACGCGCATTCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCAcg > 1:2356337/1‑71 (MQ=255) cgcgCATTCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGt < 1:750265/71‑1 (MQ=255) cgcATTCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAg < 1:1358129/48‑1 (MQ=255) gcATTCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTc < 1:2076749/71‑1 (MQ=255) cATTCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCg < 1:1883326/71‑1 (MQ=255) aTTCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAACTTAGCGCCACGCGTGAACGTTTCgg > 1:1940892/1‑71 (MQ=255) tCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGt < 1:32139/70‑1 (MQ=255) tCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGt < 1:672631/70‑1 (MQ=255) cAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGaa > 1:509871/1‑60 (MQ=255) cAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTaa > 1:374521/1‑71 (MQ=255) cAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTaa > 1:1207715/1‑71 (MQ=255) aGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAAt < 1:2662899/71‑1 (MQ=255) gCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTaa < 1:2715041/69‑1 (MQ=255) aaCTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATg > 1:1138619/1‑69 (MQ=255) tGGAATATCGTTTAGCAGAAACCCTGCGCGCACGGCTTAGCGCCAcgc > 1:503265/1‑48 (MQ=255) tGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCAcgc > 1:994969/1‑48 (MQ=255) tGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTaa < 1:2734045/64‑1 (MQ=255) ggAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTaa > 1:528320/1‑71 (MQ=255) gAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAAc > 1:1506945/1‑71 (MQ=255) gAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAAc > 1:661386/1‑71 (MQ=255) gAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAAc > 1:1838380/1‑71 (MQ=255) tCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGaa > 1:3165921/1‑46 (MQ=255) tCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAg > 1:3012595/1‑62 (MQ=255) tCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCAcc > 1:117551/1‑71 (MQ=255) tCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCAc < 1:83656/70‑1 (MQ=255) cGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCt > 1:2094414/1‑71 (MQ=255) cGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCt > 1:278823/1‑71 (MQ=255) gTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTc > 1:3293850/1‑71 (MQ=255) gTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTc > 1:2211774/1‑71 (MQ=255) gTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTc > 1:3297865/1‑71 (MQ=255) ttAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTaa > 1:1810441/1‑61 (MQ=255) tAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAAc > 1:2279537/1‑42 (MQ=255) tAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAAc > 1:1613533/1‑42 (MQ=255) tAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGa < 1:2453562/70‑1 (MQ=255) gCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGc < 1:988467/71‑1 (MQ=255) aGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCg < 1:880239/71‑1 (MQ=255) aGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCg < 1:2766424/71‑1 (MQ=255) gAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAAc > 1:1590019/1‑37 (MQ=255) gAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCg > 1:1277627/1‑70 (MQ=255) gAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCg > 1:354026/1‑70 (MQ=255) gAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCg > 1:67204/1‑70 (MQ=255) aaaCCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCGt > 1:1545598/1‑70 (MQ=255) aaCCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATg < 1:2807527/47‑1 (MQ=255) aaCCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATg < 1:705929/47‑1 (MQ=255) aaCCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATg < 1:2084581/47‑1 (MQ=255) aaCCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATg < 1:2623776/47‑1 (MQ=255) aCCCTGCGCGCACAACTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCa > 1:2141022/1‑56 (MQ=255) cccTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCg < 1:1184429/60‑1 (MQ=255) cTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGccc < 1:319577/71‑1 (MQ=255) cTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGccc < 1:1798415/71‑1 (MQ=255) cgcgcACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACt > 1:1750000/1‑71 (MQ=255) cacaACTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCa < 1:2414169/71‑1 (MQ=255) cacaACTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCa < 1:2198468/71‑1 (MQ=255) acaGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCaa > 1:3124719/1‑71 (MQ=255) acaGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCaa > 1:280801/1‑71 (MQ=255) caGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGa < 1:16424/50‑1 (MQ=255) caGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCg > 1:2954890/1‑55 (MQ=255) aGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTaa > 1:112272/1‑39 (MQ=255) gCTTATCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCAACGc < 1:1300291/71‑1 (MQ=255) gCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCAACGc > 1:544314/1‑71 (MQ=255) gCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCAACGc < 1:2630732/71‑1 (MQ=255) gCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCAACGc > 1:2535297/1‑71 (MQ=255) gCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCAACGc > 1:1902854/1‑71 (MQ=255) gCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCAACGc < 1:2248021/71‑1 (MQ=255) | CGAAGATTCATCGCGCGCAAACGCGCATTCAGCAACTGGAATATCGTTTAGCAGAAACCCTGCGCGCACAGCTTAGCGCCACGCGTGAACGTTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCAACGC > minE/1667981‑1668121 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |