| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,695,660 | C→A | 20.1% | R117L (CGC→CTC) | yfhQ ← | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,695,660 | 0 | C | A | 20.1% | 198.0 / 19.6 | 64 | R117L (CGC→CTC) | yfhQ | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (36/15); new base A (9/4); total (45/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGC > minE/1695590‑1695729 | gTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGAcgc > 1:373955/1‑71 (MQ=255) ttCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCt > 1:212631/1‑71 (MQ=255) ttCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCt > 1:593691/1‑71 (MQ=255) ttCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCt > 1:2717506/1‑71 (MQ=255) ggTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGAGCTCGCGa > 1:1214368/1‑71 (MQ=255) aGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCa < 1:482930/70‑1 (MQ=255) aGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCa < 1:449104/70‑1 (MQ=255) aGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCa > 1:1655328/1‑70 (MQ=255) ccGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAac < 1:3126556/71‑1 (MQ=255) ccGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAac < 1:2410907/71‑1 (MQ=255) aaTCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGAGCTCGCGACCAAacac > 1:1760758/1‑69 (MQ=255) aaTAGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAac > 1:1656881/1‑67 (MQ=255) aTCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAg > 1:417782/1‑71 (MQ=255) aTCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAg < 1:1735357/71‑1 (MQ=255) gcgACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGa > 1:744710/1‑57 (MQ=255) gcgACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAaca > 1:169989/1‑64 (MQ=255) gcgACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAaca > 1:1952189/1‑64 (MQ=255) gcgACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGAGCTCGCGACCaaa > 1:2050216/1‑62 (MQ=255) aTGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGAGCTcgc < 1:1400477/50‑1 (MQ=255) aTGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGAGCTcgc < 1:2903716/50‑1 (MQ=255) aTGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGAGCTcgc < 1:2276534/50‑1 (MQ=255) aTGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGAGCTcgc < 1:2276275/50‑1 (MQ=255) tGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCgg > 1:2781341/1‑71 (MQ=255) tGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCgg > 1:535829/1‑71 (MQ=255) agATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCgg > 1:1567463/2‑71 (MQ=255) gATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCggg > 1:976124/1‑71 (MQ=255) gATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCggg > 1:1039168/1‑71 (MQ=255) gATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGAGCTCGCGa > 1:610229/1‑50 (MQ=255) tAATGGAATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCgggg > 1:4483/1‑70 (MQ=255) aTGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCaaa > 1:727016/1‑51 (MQ=255) aTGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCaaa > 1:120518/1‑51 (MQ=255) ggCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCaaa > 1:210856/1‑49 (MQ=255) ggCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCaaa > 1:969232/1‑49 (MQ=255) ggCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCaaa > 1:1308527/1‑49 (MQ=255) ggCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTAttt > 1:1714670/1‑71 (MQ=255) aTTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGcc > 1:2761636/1‑71 (MQ=255) tttCTGCAACTCTTCATTGGTCAAGCCGACGCGCTcgc < 1:1231023/38‑1 (MQ=255) tttCTGCAACTCTTCATTGGTCAAGCCGACGCGCTcgc < 1:487869/38‑1 (MQ=255) tctcCAACTCTTCATTGGTCAAGCCGACGAGCTCGCGACCAAACACCAg > 1:735747/1‑49 (MQ=38) tCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGa > 1:2170875/1‑38 (MQ=255) tCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGAc > 1:2087690/1‑39 (MQ=255) tCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGAc > 1:1088859/1‑39 (MQ=255) tCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCt > 1:782215/1‑71 (MQ=255) tCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCt > 1:3198238/1‑71 (MQ=255) tGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCa < 1:2618067/39‑1 (MQ=255) tGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCa < 1:2614086/39‑1 (MQ=255) tGCAACTCTTCATTGGTCAAGCCGACGAGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCtt > 1:815283/1‑70 (MQ=255) aCTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGc < 1:10592/70‑1 (MQ=255) ctctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGa > 1:713897/1‑71 (MQ=255) tCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCt > 1:64808/1‑71 (MQ=255) cATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGc < 1:1578125/69‑1 (MQ=255) aTTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCtt < 1:2135139/70‑1 (MQ=255) ttGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCtttt < 1:1202306/71‑1 (MQ=255) ttGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCtttt < 1:889647/71‑1 (MQ=255) ttGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCtttt < 1:1076948/71‑1 (MQ=255) ggTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCtttt > 1:2341105/1‑69 (MQ=255) tCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAgg > 1:211700/1‑71 (MQ=255) tCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAgg > 1:2822608/1‑71 (MQ=255) cAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGc > 1:3160091/1‑71 (MQ=255) aaGCCGACGAGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCa > 1:811498/1‑54 (MQ=255) aaGCCGACGAGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCa > 1:1001368/1‑54 (MQ=255) aaGCCGACGAGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCa > 1:2169880/1‑54 (MQ=255) aGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCg > 1:300603/1‑71 (MQ=255) gCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCg > 1:1566040/1‑70 (MQ=255) ccGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCa > 1:525311/1‑71 (MQ=255) gcgcTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTcgc > 1:1986052/1‑71 (MQ=255) gcgcTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTcgc > 1:434225/1‑71 (MQ=255) gcgcTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTcgc > 1:3199907/1‑71 (MQ=255) gcgcTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTcgc > 1:1334698/1‑71 (MQ=255) | GTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGC > minE/1695590‑1695729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |