Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 204,556 A→G 100% intergenic (+156/‑28) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported204,5560AG100.0% 29.7 / NA 12intergenic (+156/‑28)alaV/rrlHtRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/7);  total (5/7)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACG  >  Exported/204492‑204606
                                                                |                                                  
aaaTTGAAACACTGAACAATGAAAGTTGTTCGTGATTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                               >  1:222814/1‑70 (MQ=11)
aaaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                               >  1:115395/1‑70 (MQ=255)
aaaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                               >  1:410313/1‑70 (MQ=255)
  aTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAg                                            <  1:128892/71‑1 (MQ=255)
                            ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg                                 <  1:105245/56‑1 (MQ=255)
                            ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAg                  <  1:58446/71‑1 (MQ=21)
                                   gTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTaa           <  1:194584/71‑1 (MQ=21)
                                    tctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAg          <  1:375107/71‑1 (MQ=21)
                                          aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg        <  1:449069/67‑1 (MQ=25)
                                          aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca    >  1:303380/1‑71 (MQ=21)
                                            aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACg  >  1:178238/1‑71 (MQ=21)
                                                    aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                 <  1:486509/48‑1 (MQ=255)
                                                                |                                                  
AAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACG  >  Exported/204492‑204606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: