New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP009048 4132585 =86 (1.060)3 (0.040) 3/82 NT 3.7% coding (760/879 nt) php predicted hydrolase
?AP009048 4132618 = 83 (1.220)coding (793/879 nt) php predicted hydrolase
Rejected: Frequency below/above cutoff threshold.

CACATCGGCCTGACTGAATCCTGACTGGCGCAATTGCGGAATAAAGGTGGTTAGTAAATAGTCATAGCCATAACCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AP009048/4132662‑4132585
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑taaccaccTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTCAGGCCGATGTGGATGTGATGTTACGTGAAAATCCCT  >  AP009048/4132618‑4132687
                                                                                                                                                    
CACATCGGCCTGACTGAATCCTGACTGGCGCAATTGCGGAATAAAGGTGGTTAGTAAATAGTCATAGCCAT                                                                               <  1:3163047/71‑1
CACATCGGCCTGACTGAATCCTGACTGGCGCAATTGCGGAATAAAGGTGGTTAGTAAATAGTCATAGCCAT                                                                               <  1:6971747/71‑1
 ACATCGGCCTGACTGAATCCTGACTGGCGCAATTGCGGAATAAAGGTGGTTAGTAAATAGTCATAGCCAT                                                                               >  1:2562792/1‑70
   ATCGGCCTGACTGAATCCTGACTGGCGCAATTGCGGAATAAAGGTGGTTAGTAAATAGTCATAGCCATAAC                                                                            >  1:5441082/1‑71
      GGCCTGACTGAATCCTGACTGGCGCAATTGCGGAATAAAGGTGGTTAGTAAATAGTCATAGCCATAACCA                                                                          <  1:7623509/70‑1
       GCCTGACTGAATCCTGACTGGCGCAATTGCGGAATAAAGGTGGTTAGTAAATAGTCATAGCCATAACCACC                                                                        <  1:2113673/71‑1
             CTGAATCCTGACTGGCGCAATTGCGGAATAAAGGTGGTTAGTAAATAGTCATAGCCATA                                                                              <  1:5217443/59‑1
                            CGCAATTGCGGAATAAAGGTGGTTAGTAAATAGTCATAGCCAT                                                                               <  1:5101431/43‑1
                               AATTGCGGAATAAAGGTGGTTAGTAAATAGTCATAGCCATAA                                                                             >  1:578135/1‑42
                               AATTGCGGAATAAAGGTGGTTAGTAAATAGTCATAGCCATAA                                                                             >  1:3046784/1‑42
                               AATTGCGGAATAAAGGTGGTTAGTAAATAGTCATAGCCATAA                                                                             >  1:3963381/1‑42
                               AATTGCGGAATAAAGGTGGTTAGTAAATAGTCATAGCCATAA                                                                             >  1:1559586/1‑42
                               AATTGCGGAATAAAGGTGGTTAGTAAATAGTCATAGCCAT                                                                               <  1:7001858/40‑1
                               AATTGCGGAATAAAGGTGGTTAGTAAATAGTCATAGCCAT                                                                               <  1:4785986/40‑1
                                 TTGCGCAATAAAGGTGGTTAGTAAATAGTCATAGCCAT                                                                               <  1:2135281/38‑1
                                           AAGGTGGTTAGTAAATAGTCATAGCCATAACCACCTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTCAG                                    >  1:1310804/1‑71
                                               TGGTTAGTAAATAGTCATAGCCATAACCACCTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTCAGGCCG                                <  1:875508/71‑1
                                                GGTTAGGAAATAGTCATAGCCATAACCACCTTTATTCCGCAATTGC                                                        >  1:128/1‑46
                                                                      TAACCACCTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTCAGGCCGATGTGGATGTGATGTTACGTGA          >  1:6267994/1‑70
                                                                      TAACCACCTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTCAGGCCGATGTGGATGT                      <  1:2872744/58‑1
                                                                      TAACCACCTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTCAGGCCGATGTGGATGT                      <  1:4151852/58‑1
                                                                      TAACCACCTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTCAGGCCGATGTGGATGT                      <  1:1923979/58‑1
                                                                      TAACCACCTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTCAGGCCGATGTGG                          >  1:6619629/1‑54
                                                                      TAACCACCTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTCAGGCCGATGTGG                          >  1:7290107/1‑54
                                                                       AACCACCTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTCAGGCCGA                               <  1:1227016/48‑1
                                                                       AACCACCTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTCAGGCCGA                               <  1:2724335/48‑1
                                                                        ACCACCTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTCAGGCCGATGTGGATGTGATGTTACGTGAAAA       >  1:1425862/1‑71
                                                                        ACCACCTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTCAGGCCGA                               <  1:4471108/47‑1
                                                                        ACCACCTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTC                                      <  1:3529879/40‑1
                                                                        ACCACCTTTATTCCGCAATTGCGCCAGTCAGGATT                                           <  1:3676388/35‑1
                                                                        ACCACCTTTATTCCGCAATTGCGCCAGTCAGGATT                                           <  1:190460/35‑1
                                                                        ACCACCTTTATTCCGCAATTGCGCCAGTCAGGATT                                           <  1:1236561/35‑1
                                                                          CACCTTTATTCCGCAATTGCGCCAGTCAGGATTAAGTCAGGCCGATGTGGATGTGAT                   >  1:383719/1‑57
                                                                           ACCTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTCAGGCCGATGTGGATGTGATGTTACGTGAAAATCC    <  1:3780885/71‑1
                                                                            CCTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTCAGGCCGATGTGGATGT                      <  1:2385867/52‑1
                                                                             CTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTCAGGCCGATGTGGATGTGATGTTACGTGAAAATCCCT  >  1:7291787/1‑71
                                                                             CTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTCAGGCCGATGTGGATGTGATG                  >  1:1958138/1‑55
                                                                                                                                                    
CACATCGGCCTGACTGAATCCTGACTGGCGCAATTGCGGAATAAAGGTGGTTAGTAAATAGTCATAGCCATAACCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AP009048/4132662‑4132585
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑taaccaccTTTATTCCGCAATTGCGCCAGTCAGGATTCAGTCAGGCCGATGTGGATGTGATGTTACGTGAAAATCCCT  >  AP009048/4132618‑4132687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.