New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP009048 4196939 =134 (1.650)3 (0.040) 3/90 NT 2.1% coding (621/1332 nt) secY preprotein translocase membrane subunit
?AP009048 4196974 = 160 (2.140)coding (656/1332 nt) secY preprotein translocase membrane subunit
Rejected: Frequency below/above cutoff threshold.

CGTCACTGCAAATACTAATACTGCAACCAACAGCAACACGAGGAAGTGCAGGTCGCCTTGACGCGCTTGCTCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AP009048/4197011‑4196939
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctcgTGTTGCTGTTGGTTGCAGTATTAGTATTTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACG  >  AP009048/4196974‑4197043
                                                                                                                                               
CGTCACTGCAAATACTAATACTGCAACCAACAGCAACACGAGGAAGTGCAGGTCGCCTTGACGCGCTTGCT                                                                          <  1:2328490/71‑1
 GTCACTGCAAATACTAATACTGCAACCAACAGCAACACGAGGAAGTGCAGGTCGCCTTGACGCGCTTGCTC                                                                         >  1:1734611/1‑71
 GTCACTGCAAATACTAATACTGCAACCAACAGCAACACGAGGAAGTGCAGGTCGCCTTGACGCGCTTGCTC                                                                         >  1:6926055/1‑71
 GTCACTGCAAATACTAATACTGCAACCAACAGCAACACGAGGAAGTGCAGGTCGCCTTGACGCGCTTGCTC                                                                         >  1:2199883/1‑71
 GTCACTGCAAATACTAATACTGCAACCAACAGCAACACGAGGAAGTGCAGGTCGCCTTGACGCGCTTGCTC                                                                         >  1:2993303/1‑71
                                         GGAAGTGCAGGTCGCCTTGACGCGCTTGCTCGTGTTGCTGTTGGTTGCAGTATTAGTATTTGCAGTGACGT                                 >  1:2079860/1‑71
                                          GAAGTGCAGGTCGCCTTGACGCGCTTGCTCGTGTTGCTGTTGGTTGCAGT                                                     >  1:3620303/1‑50
                                            AGTGCAGGTCGCCTTGACGCGCTTGCTCGTGTTGCTGTTGGTTGCAG                                                      >  1:2415998/1‑47
                                                                   taCTCGTGTTGCTGTTGGTTGCAGTATTAGTATTTGCAGTGACGTTCTTTGTTGTATTTGT                 >  1:3352189/3‑61
                                                                     CTCGTGTTGCTGTTGGTTGCAGTATTAGTATTTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCA     <  1:2367129/71‑1
                                                                     CTCGTGTTGCTGTTGGTTGCAGTATTAGTATTTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCA     <  1:7524769/71‑1
                                                                       CGTGTTGCTGTTGGTTGCAGTATTAGTATTTGCAGTGACGTTCT                              <  1:4611952/44‑1
                                                                       CGTGTTGCTGTTGGTTGCAGTATTAGTATTTGCAGTGACG                                  <  1:4561974/40‑1
                                                                       CGTGTTGCTGTTGGTTGCAGTATTAGTATTTGCAGTGACG                                  <  1:2042648/40‑1
                                                                       CGTGTTGCTGTTGGTTGCAGTATTAGTATTTGCAGTGACG                                  <  1:7883575/40‑1
                                                                        GTGTTGCTGTTGGTTGCAGTATTAGTATTTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACG  <  1:1406793/71‑1
                                                                        GTGTTGCTGTTGGTTGCAGTATTAGTATTTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACG  <  1:6072629/71‑1
                                                                        GTGTTGCTGTTGGTTGCAGTATTAGTATTTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACG  <  1:679944/71‑1
                                                                                                                                               
CGTCACTGCAAATACTAATACTGCAACCAACAGCAACACGAGGAAGTGCAGGTCGCCTTGACGCGCTTGCTCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AP009048/4197011‑4196939
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctcgTGTTGCTGTTGGTTGCAGTATTAGTATTTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACG  >  AP009048/4196974‑4197043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.