New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP009048 4462605 =105 (1.290)4 (0.060) 3/84 NT 5.0% intergenic (‑60/‑34) yjgA/pmbA conserved hypothetical protein/predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17
?AP009048 4462627 = 63 (0.900)intergenic (‑82/‑12) yjgA/pmbA conserved hypothetical protein/predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17
Rejected: Frequency below/above cutoff threshold.

CTGCGCTTCAACTTGAGAGATTACTTTCATTGCAAGTGCCATAATGTAGAGAGTTTCTCTGAAGTCTAACAGAGAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AP009048/4462680‑4462605
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagagaaACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAG  >  AP009048/4462627‑4462696
                                                                                                                                                  
CTGCGCTTCAACTTGAGAGATTACTTTCATTGCAAGTGCCATAATGTAGAGAGTTTCTCTGAAGTCTAACA                                                                             <  1:5002525/71‑1
CTGCGCTTCAACTTGAGAGATTACTTTCATTGCAAGTGCCATAATGTAGAGAGTTTCTCTGAAGTCTAACA                                                                             <  1:2210453/71‑1
  tctCTTCAACTTGAGAGATTACTTTCATTGCAAGTGCCATAATGTAGAGAGTTTCTCTGAAGTCTAACAG                                                                            <  1:822715/67‑1
  GCGCTTCAACTTGAGAGATTACTTTCATTGCAAGTGCCATAATGTAGAGAGTTTCTCTGAAGTCTAACAG                                                                            <  1:354431/70‑1
  GCGCTTCAACTTGAGAGATTACTTTCATTGCAAGTGCCATAATGTAGAGAGTTTCTCTGAAGTCTAACA                                                                             >  1:3881503/1‑69
    GCTTCAACTTGAGAGATTACTTTCATTGCAAGTGCCATAATGTAGAGAGTTTCTCTGAAGTCTAACAGAG                                                                          >  1:694690/1‑70
    GCTTCAACTTGAGAGATTACTTTCATTGCAAGTGCCATAATGTAGAGAGTTTCTCTGAAGTCTAACAGAG                                                                          <  1:592139/70‑1
     CTTCAACTTGAGAGATTACTTTCATTGCAAGTGCCATAATGTAGAGAGTTTCTCTGAAGTCTAACAGA                                                                           >  1:1876744/1‑68
        CAACTTGAGAGATTACTTTCATTGCAAGTGCCATAATGTAGAGAGTTTCTCTGAAGTCTAAC                                                                              >  1:1702772/1‑62
         AACTTGAGAGATTACTTTCATTGCAAGTGCCATAATGTAGAGAGTTTCTCTGAAGTCTAACA                                                                             >  1:739614/1‑62
         AACTTGAGAGATTACTTTCATTGCAAGTGCCATAATGTAGAGAGTTTCTCTGAAGTCTAACA                                                                             >  1:5958133/1‑62
           CTTGAGAGATTACTTTCATTGCAAGTGCCATAATGTAGAGAGTTTCTCTGAAGTCTAACAG                                                                            <  1:612670/61‑1
           CTTGAGAGATTACTTTCATTGCAAGTGCCATAATGTAGAGAGTTTCTCTGAAGTCTAACAG                                                                            <  1:6574486/61‑1
                GAGATTACTTTCATTGCAAGTGCCATAATGTAGAGAGTTTCTCTGAAGTCTAAC                                                                              >  1:1739068/1‑54
                GAGATTACTTTCATTGCAAGTGCCATAATGTAGAGAGTTTCTCTGAAGTCTAAC                                                                              >  1:5864353/1‑54
                      ACTTTCATTGCAAGTGCCATAATGTAGAGAGTTTCTCTGAAGTCTAACAGAGA                                                                         <  1:7230919/53‑1
                              TGCAAGTGCCATAATGTAGAGAGTTTATCTGAAGTCTAACAGAGca                                                                        >  1:2125635/1‑44
                                                    GTTTCTCTGAAGTCTAACAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTG                          >  1:4187379/1‑70
                                                       TCTCTGAAGTCTAACAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCA                               >  1:1777612/1‑62
                                                       TCTCTGAAGTCTAACAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCA                               >  1:7091893/1‑62
                                                        CTCTGAAGTCTAACAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTC                                <  1:2129840/60‑1
                                                                     CAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTC        >  1:172274/1‑71
                                                                     CAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGAT          <  1:4544144/69‑1
                                                                      AGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCA                   <  1:484931/59‑1
                                                                        AGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTG      >  1:3576500/1‑70
                                                                           AACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAG  <  1:3844644/71‑1
                                                                                                                                                  
CTGCGCTTCAACTTGAGAGATTACTTTCATTGCAAGTGCCATAATGTAGAGAGTTTCTCTGAAGTCTAACAGAGAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AP009048/4462680‑4462605
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagagaaACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAG  >  AP009048/4462627‑4462696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.