| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 75,753 | A→C | 5.6% | L516W (TTG→TGG) | sgrR ← | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 75,753 | 0 | A | C | 5.6% | 230.0 / 6.1 | 87 | L516W (TTG→TGG) | sgrR | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (30/52); new base C (3/2); total (33/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGC > AP009048/75686‑75822 | ccAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCaa > 1:6800218/1‑69 (MQ=255) cAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCaataa < 1:6878711/71‑1 (MQ=255) gCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATg > 1:7548464/1‑71 (MQ=255) ccGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATgg < 1:6732549/71‑1 (MQ=255) gggTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCAc > 1:7073934/1‑70 (MQ=255) ggTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCCATTATTGCACCa < 1:2615056/71‑1 (MQ=255) ggTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCCATTATTGCACCa < 1:1609628/71‑1 (MQ=255) gTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCAt < 1:5748295/71‑1 (MQ=255) aTTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCg > 1:4216873/1‑71 (MQ=255) ttCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCaataat > 1:742786/1‑60 (MQ=255) ttCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCg < 1:1080724/70‑1 (MQ=255) tCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCaata < 1:3459127/57‑1 (MQ=255) cATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCtt > 1:809611/1‑71 (MQ=255) cATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCtt > 1:5578801/1‑71 (MQ=255) cATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCtt > 1:4388715/1‑71 (MQ=255) cATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCt < 1:4460641/70‑1 (MQ=255) cATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCt < 1:2221445/70‑1 (MQ=255) aTGCGCGGCCTGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCttt > 1:3638427/1‑71 (MQ=255) aTGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCttt > 1:2460333/1‑71 (MQ=255) aTGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCtt < 1:6830172/70‑1 (MQ=255) aTGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCtt < 1:1666266/70‑1 (MQ=255) tGCGCGGCCTGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATgg > 1:1077611/1‑58 (MQ=255) tGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTg < 1:3875815/71‑1 (MQ=255) gcgcAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGc < 1:2491260/58‑1 (MQ=255) cgcAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCaat > 1:7709317/1‑51 (MQ=255) cgcAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCttt < 1:7496157/68‑1 (MQ=255) cgcAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCttt < 1:2760387/68‑1 (MQ=255) cgcAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCttt < 1:7486703/68‑1 (MQ=255) gcAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTg < 1:7251755/71‑1 (MQ=255) gcAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTg < 1:3870018/71‑1 (MQ=255) gcAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTg < 1:6202147/71‑1 (MQ=255) gcAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTg < 1:2187251/71‑1 (MQ=255) gcAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTg > 1:2762508/1‑71 (MQ=255) gcAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTg < 1:2630400/71‑1 (MQ=255) ggCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCa < 1:2942866/45‑1 (MQ=255) ggCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCa < 1:3050710/45‑1 (MQ=255) ggCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCa < 1:4170588/45‑1 (MQ=255) ggCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCg < 1:4500778/71‑1 (MQ=255) gCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCaataat < 1:4008370/49‑1 (MQ=255) gCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCaat < 1:1824532/46‑1 (MQ=255) gCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCAcc < 1:2779730/55‑1 (MQ=255) gCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCAcc < 1:246369/55‑1 (MQ=255) cgcgCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGAcc < 1:6359796/71‑1 (MQ=255) gcgcATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGAc > 1:4856983/1‑69 (MQ=255) gcATACTGCGTTGCCCCTGAATGCTCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGt < 1:7203392/71‑1 (MQ=255) cATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCt > 1:2731250/1‑60 (MQ=255) cATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGAc > 1:3176171/1‑66 (MQ=255) cATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGAc > 1:6628406/1‑66 (MQ=255) cATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGtt < 1:5069654/71‑1 (MQ=255) cATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGtt > 1:4511371/1‑71 (MQ=255) cTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCt < 1:6697328/70‑1 (MQ=255) cTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTg < 1:7547189/71‑1 (MQ=255) cTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTg < 1:5487221/71‑1 (MQ=255) tGCGTTGCCCCTGAATGATCAGCCAGTGGTGCaataa > 1:5310431/1‑37 (MQ=255) tGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAg > 1:3104677/1‑64 (MQ=255) ttGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGAcc < 1:5145577/58‑1 (MQ=255) ttGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCAcc < 1:785689/71‑1 (MQ=255) tGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGc < 1:5289485/49‑1 (MQ=255) tGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCAc > 1:1608540/1‑69 (MQ=255) gCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGc > 1:1905806/1‑48 (MQ=255) ccccTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGc < 1:1873544/47‑1 (MQ=255) ccccTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTg > 1:7436635/1‑49 (MQ=255) ccccTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGt < 1:3189990/71‑1 (MQ=255) ccccTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGt < 1:4833361/71‑1 (MQ=255) cccTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGc < 1:7343092/64‑1 (MQ=255) cccTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGc < 1:6578629/64‑1 (MQ=255) acTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGc > 1:658808/2‑49 (MQ=255) tGAATGATCAGCCAGTGGTGCAATAATGGGACCATCGCTTTGCTGGCGACCa < 1:2925440/52‑1 (MQ=255) tGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGtt < 1:2119949/55‑1 (MQ=255) tGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGtt < 1:239497/55‑1 (MQ=255) aaTGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGc < 1:3804604/55‑1 (MQ=255) aaTGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCa > 1:5694171/1‑60 (MQ=255) aaTGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGc < 1:112404/69‑1 (MQ=255) aaTGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACAAGTTGc < 1:1017543/55‑1 (MQ=255) aTGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCATTTGCTGGCACCAGTTCGCCAg < 1:2598686/71‑1 (MQ=255) aTGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGc < 1:3351573/54‑1 (MQ=255) aTGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGc > 1:286389/1‑68 (MQ=255) aTGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAg < 1:2838987/71‑1 (MQ=255) aTGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAg < 1:5900631/71‑1 (MQ=255) tGATCAGCCAGTGGTGCAATAATGGCACGATCGCTTTGCTGGCGACCAGtgg > 1:6457474/1‑50 (MQ=39) tGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTc > 1:1601805/1‑65 (MQ=255) tGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGc > 1:885734/1‑67 (MQ=255) tGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGc > 1:808503/1‑67 (MQ=255) gATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCt < 1:1823851/53‑1 (MQ=255) aTCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGAt < 1:1133288/70‑1 (MQ=255) tCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTc < 1:4782687/71‑1 (MQ=255) tCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTc < 1:251237/71‑1 (MQ=255) aGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCa < 1:5730817/70‑1 (MQ=255) aGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCAt > 1:2980817/1‑71 (MQ=255) tggtgCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGtt < 1:6363266/53‑1 (MQ=255) tggtgCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGcc < 1:700390/57‑1 (MQ=255) ggtgCCATTATTGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGcc > 1:6797929/1‑70 (MQ=255) gtgCCATTATTGCACCATCGCTTTGCTGGCGACCAGTTg > 1:305866/1‑39 (MQ=25) gtgCCATTATTGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCAt > 1:2093876/1‑71 (MQ=255) gtgCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTc > 1:6042433/1‑52 (MQ=255) gCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTg > 1:6047578/1‑71 (MQ=255) cAATAATGGCACCATCGCTTTGCTGGCGACCAGtt < 1:7636995/35‑1 (MQ=255) cAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTgc > 1:511097/1‑71 (MQ=255) aataatGGCACCATCGCTTTGCTGGAGACCAGTTGCTGGCACCAg > 1:1583328/1‑45 (MQ=255) | CCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGC > AP009048/75686‑75822 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |