| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 2,132,042 | T→A | 6.8% | D325V (GAC→GTC) | wcaD ← | predicted colanic acid polymerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 2,132,042 | 0 | T | A | 6.8% | 322.1 / 13.2 | 117 | D325V (GAC→GTC) | wcaD | predicted colanic acid polymerase |
| Reads supporting (aligned to +/- strand): ref base T (43/66); new base A (6/2); total (49/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.68e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
CCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGTGAGCCAAA > AP009048/2131973‑2132112 | cccATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTc < 1:4375625/71‑1 (MQ=255) cATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTa < 1:3636939/71‑1 (MQ=255) aTGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTAt > 1:3312240/1‑71 (MQ=255) aTGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTAt > 1:4674114/1‑71 (MQ=255) tGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATg > 1:2265734/1‑71 (MQ=255) aCCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTc < 1:2112846/64‑1 (MQ=255) aCCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGtt > 1:7868667/1‑71 (MQ=255) ccACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGtt < 1:292320/70‑1 (MQ=255) acaGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAACCCATTGTCTATGGtttt < 1:7782127/70‑1 (MQ=255) gTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCg < 1:1592781/71‑1 (MQ=255) gATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGtttt < 1:3725786/65‑1 (MQ=255) aTAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACa < 1:1251562/71‑1 (MQ=255) aTAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACa < 1:6821477/71‑1 (MQ=255) aaaaaCACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATc > 1:4903912/1‑71 (MQ=255) aaaaaCACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATc > 1:4393406/1‑71 (MQ=255) aaaCACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTcc < 1:496102/63‑1 (MQ=255) aaCACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGc < 1:2247013/71‑1 (MQ=255) cacCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGttttt < 1:7760597/58‑1 (MQ=255) cacCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCAc > 1:1527612/1‑71 (MQ=255) acCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCAcc > 1:7390172/1‑71 (MQ=255) ccGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCAcc < 1:6704634/70‑1 (MQ=255) ccGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCg < 1:4873065/71‑1 (MQ=255) cgcgAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGAc > 1:891136/1‑60 (MQ=255) cgcgAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACa < 1:259865/61‑1 (MQ=255) cgcgAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGt < 1:6829592/71‑1 (MQ=255) gcgAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATTGTTTTTCCGACATCCGCACCGtt < 1:6409211/71‑1 (MQ=255) gcgAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGtt < 1:3768782/71‑1 (MQ=255) gAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTc < 1:4950666/53‑1 (MQ=255) aaCCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCAc < 1:7189474/64‑1 (MQ=255) aCCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTaaa > 1:3623947/1‑70 (MQ=255) cAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAatat < 1:2688317/71‑1 (MQ=255) aGGAAAAATAAATAATCAGCAGATACAAACCATTGTCt > 1:884881/1‑38 (MQ=255) aGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATAtt < 1:7504274/71‑1 (MQ=255) gAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTaa < 1:232927/64‑1 (MQ=255) gAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTcc > 1:5899788/1‑71 (MQ=255) gAAAAATAAATAATCAGCAGATACAAACCATAGACAATGGt > 1:6849481/1‑41 (MQ=25) gAAAAATAAATAATCAGCAGATACAAACCATAGACAATGGt > 1:2551503/1‑41 (MQ=25) gAAAAATAAATAATCAGCAGATACAAACCATAGACAATGGt > 1:3871945/1‑41 (MQ=25) aaaaaTAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCg < 1:1183524/71‑1 (MQ=255) aaaaaTAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCg < 1:7813022/71‑1 (MQ=255) aaaaTAAATAATCAGCATATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTc < 1:6973641/68‑1 (MQ=255) aaaaTAAATAATCAGCAGATACAAACCATTGTCTAt > 1:1595862/1‑36 (MQ=255) aaaaTAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGtt > 1:3257898/1‑60 (MQ=255) aaaaTAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATAtt < 1:7081431/67‑1 (MQ=255) aaaaTAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGa < 1:5846635/71‑1 (MQ=255) aaataaatAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGaa > 1:4209092/1‑71 (MQ=255) ataaataaTCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATc < 1:3640064/48‑1 (MQ=255) ataaataaTCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTa < 1:3069071/58‑1 (MQ=255) taaataaTCAGCAGATACAAACCATTGTCTTTGGTTTTTCCGACATCCGCACCGTTa < 1:3324015/57‑1 (MQ=255) ataatCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGc > 1:1082683/1‑71 (MQ=255) taatCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGc > 1:156481/1‑70 (MQ=255) agcagATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGa > 1:7711657/1‑67 (MQ=255) gcagATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACatat < 1:7388549/71‑1 (MQ=255) agATACAAACCATTGTCTATGGTTTTTCCGACATcc < 1:7400778/36‑1 (MQ=255) agATACAAACCATT‑TCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCa > 1:7013265/1‑71 (MQ=255) gATACAGACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATAtt > 1:1020373/1‑50 (MQ=255) gATACAAACCATTGTCTATGGTTTTTCCGACATCCGc > 1:4906023/1‑37 (MQ=255) gATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTaa < 1:3844485/45‑1 (MQ=255) gATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATAtt > 1:2813243/1‑50 (MQ=255) aTACAAACCATTGTCTATGGTTTTTCCGCCATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCa > 1:3159121/1‑70 (MQ=255) aTACAAACCATTGTCTATGGTTTTTCCGACATCCGc < 1:2314345/36‑1 (MQ=255) aTACAAACCATTGTCTATGGTTTTTCCGACATCCGc < 1:2336324/36‑1 (MQ=255) aTACAAACCATTGTCTATGGTTTTTCCGACATCCGCAc > 1:4032211/1‑38 (MQ=255) aTACAAACCATTGTCTATGGTTTTTCCGACATCCGCAc > 1:5865505/1‑38 (MQ=255) aTACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGa < 1:3941636/62‑1 (MQ=255) tACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTaaa < 1:7500150/44‑1 (MQ=255) aCAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTaaa < 1:114641/43‑1 (MQ=255) aCAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTaaa < 1:1627961/43‑1 (MQ=255) aCAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATa > 1:7709138/1‑70 (MQ=255) cAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAat > 1:2190207/1‑43 (MQ=255) cAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATa > 1:1440963/1‑69 (MQ=255) cAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATa > 1:1413066/1‑69 (MQ=255) cAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATa > 1:4978071/1‑69 (MQ=255) aaaaCCATAGACAATGGTTTTTCCGACATCCGAACCGTTAAATATTCCGAATGATGCGACATACTCATaa < 1:1012166/69‑1 (MQ=37) aaaCCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAat < 1:5520678/51‑1 (MQ=255) aaaCCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACatat < 1:7146331/63‑1 (MQ=255) aaCCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGt < 1:6126730/71‑1 (MQ=255) aaCCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGt < 1:5011014/71‑1 (MQ=255) aaCCATAGACAATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGt > 1:2943038/1‑71 (MQ=255) aaCCATAGACAATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGt > 1:1234661/1‑71 (MQ=255) aCCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAatga > 1:3905560/1‑51 (MQ=255) aCCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGt > 1:7126892/1‑70 (MQ=255) aCCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGTg < 1:7831205/71‑1 (MQ=255) aCCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGTg < 1:4213394/71‑1 (MQ=255) aCCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGTg < 1:4513645/71‑1 (MQ=255) aCCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGTg < 1:3224193/71‑1 (MQ=255) aCCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGTg < 1:3551191/71‑1 (MQ=255) ccATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAg > 1:6895349/1‑68 (MQ=255) ccATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGt > 1:1001865/1‑69 (MQ=255) ccATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGt > 1:4960397/1‑69 (MQ=255) ccATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGt > 1:4473159/1‑69 (MQ=255) ccATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGTGa < 1:4609703/71‑1 (MQ=255) cATTGTCTATGGTTTTTCCGACATCCGCACCGTTaaa < 1:6878025/37‑1 (MQ=255) cATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATAtt < 1:2990989/41‑1 (MQ=255) aTTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGTGAGc < 1:7738393/71‑1 (MQ=255) ttGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAat < 1:3250845/45‑1 (MQ=255) ttGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGc < 1:5630100/50‑1 (MQ=255) ttGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACatat > 1:6215813/1‑57 (MQ=255) tCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAatg < 1:2632786/43‑1 (MQ=255) tCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGTGAGCCaaa > 1:4656689/1‑71 (MQ=255) | CCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGTGAGCCAAA > AP009048/2131973‑2132112 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |