Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AP009048 2,170,610 T→C 8.2% intergenic (‑167/‑239) yegR ← / → yegS hypothetical protein/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0090482,170,6100TC 8.2% 169.3 / 6.1 61intergenic (‑167/‑239)yegR/yegShypothetical protein/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/22);  new base C (2/3);  total (36/25)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

TACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGG  >  AP009048/2170542‑2170680
                                                                    |                                                                      
tACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCttt                                                                        <  1:885804/69‑1 (MQ=255)
tACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCttt                                                                        <  1:7617391/69‑1 (MQ=255)
tACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCttt                                                                        <  1:1481611/69‑1 (MQ=255)
  caacaTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAata                                                                    <  1:6023205/71‑1 (MQ=255)
    acaTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAatata                                                                  <  1:6625527/71‑1 (MQ=255)
      aattCCTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTCAata                                                                    >  1:3727893/3‑67 (MQ=38)
      aTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAatatat                                                                 >  1:5972015/1‑70 (MQ=255)
       tttCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAAtatatta                                                               <  1:5123045/71‑1 (MQ=255)
           cTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATatattatatt                                                           >  1:3155730/1‑71 (MQ=255)
           cTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATatattatatt                                                           >  1:644117/1‑71 (MQ=255)
              cATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTCAATataatatattaa                                                         <  1:3678011/70‑3 (MQ=255)
               aTAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATTGGGGCTTCAATATAATATATTAAAg                                                       <  1:410343/71‑1 (MQ=38)
                 aaGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTa                                                                       >  1:5385091/1‑53 (MQ=255)
                  aGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGAt                                                     <  1:5883924/70‑1 (MQ=255)
                   gTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTCAatata                                                                  <  1:5270074/56‑1 (MQ=255)
                     aGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCa                                                 >  1:7848217/1‑71 (MQ=255)
                       gAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACt                                               >  1:923258/1‑71 (MQ=255)
                         aTTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGt                                             >  1:6718895/1‑71 (MQ=255)
                          ttACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATg                                                    >  1:5568828/1‑63 (MQ=255)
                            aCGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGtt                                            >  1:6249668/1‑69 (MQ=255)
                             cGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAata                                                                    >  1:2044421/1‑44 (MQ=255)
                             cGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGttt                                           <  1:4304565/69‑1 (MQ=255)
                             cGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTa                                          <  1:5575695/70‑1 (MQ=255)
                                aCATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAAtatatta                                                               >  1:438269/1‑46 (MQ=255)
                                aCATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGt                                             >  1:218511/1‑64 (MQ=255)
                                aCATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGtt                                       >  1:2094511/1‑70 (MQ=255)
                                aCATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGtc                                       >  1:5830893/1‑69 (MQ=255)
                                   ttGAGGCCCTTCAGGGTAACTCAATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGaa                                    >  1:6258997/1‑70 (MQ=255)
                                    tGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAata                                  >  1:1113168/1‑71 (MQ=255)
                                     gAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAatat                                 >  1:1356715/1‑71 (MQ=255)
                                     gAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAatat                                 >  1:4756836/1‑71 (MQ=255)
                                       ggCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAatat                                 >  1:4879992/1‑69 (MQ=255)
                                          ccTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGt                            <  1:2778131/71‑1 (MQ=255)
                                           cTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGa                                                         >  1:1347590/1‑41 (MQ=255)
                                           cTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGta                           >  1:1289988/1‑71 (MQ=255)
                                           cTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGta                           >  1:814115/1‑71 (MQ=255)
                                            ttCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAatat                                 <  1:823783/64‑1 (MQ=255)
                                              cAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTatat                        >  1:6409408/1‑71 (MQ=255)
                                               aGGGTAACTCCATGGGGGCTTTAATatattatatt                                                           <  1:2921859/35‑1 (MQ=255)
                                               aGGGTAACTCCATGGGGGCTTTAATatattatatt                                                           <  1:306103/35‑1 (MQ=255)
                                               aGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATg                       >  1:1589802/1‑71 (MQ=255)
                                                gggTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACt                                               >  1:7162889/1‑46 (MQ=255)
                                                  gTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGtt                                            <  1:4955150/47‑1 (MQ=255)
                                                   tAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGctct                   >  1:4640481/1‑71 (MQ=255)
                                                    aaCTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCtt                  >  1:2444663/1‑71 (MQ=255)
                                                     aCTCCATGGGGGCTTTAATATATTATATTGAAGATGACACTGTTTAGTTGAATATTAGGTATATGCTCttt                 >  1:2613173/1‑71 (MQ=255)
                                                      cTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCtttt                >  1:3490269/1‑71 (MQ=255)
                                                       tCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTg                                      <  1:4536045/48‑1 (MQ=255)
                                                       tCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTg                                      <  1:1721304/48‑1 (MQ=255)
                                                        ccATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTg                                      <  1:1080578/47‑1 (MQ=255)
                                                        ccATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCtttttt              >  1:6412806/1‑71 (MQ=255)
                                                         cATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTg             <  1:1943128/71‑1 (MQ=255)
                                                         cATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTg             <  1:1502133/71‑1 (MQ=255)
                                                          aTGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGa            <  1:5118431/71‑1 (MQ=255)
                                                          aTGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGa            <  1:2301486/71‑1 (MQ=255)
                                                           tGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTa                               <  1:2543138/51‑1 (MQ=255)
                                                             ggggCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTa                               >  1:7018630/1‑49 (MQ=255)
                                                             ggggCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAgg                             >  1:6019359/1‑51 (MQ=255)
                                                             ggggCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAgg                             >  1:6840586/1‑51 (MQ=255)
                                                               ggCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAAttt       <  1:4213464/71‑1 (MQ=255)
                                                               atcTTCAATATAATATATTAAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAAtt        >  1:6767541/3‑70 (MQ=38)
                                                                gCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTa      >  1:4802383/1‑71 (MQ=255)
                                                                gCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTa      <  1:2118968/71‑1 (MQ=255)
                                                                 cTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTAt     <  1:6802723/71‑1 (MQ=255)
                                                                    tAATATATTATATTGAAGATGCCACTGTTTAGTTGa                                     >  1:6298123/1‑36 (MQ=255)
                                                                    tAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCttt                 <  1:2198914/56‑1 (MQ=255)
                                                                    tAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCgg  >  1:2580560/1‑71 (MQ=255)
                                                                    tAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCgg  >  1:2752077/1‑71 (MQ=255)
                                                                    tAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCg   <  1:5134484/70‑1 (MQ=255)
                                                                    |                                                                      
TACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGG  >  AP009048/2170542‑2170680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: