| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 2,176,543 | C→G | 5.9% | L53F (TTG→TTC) | gatB ← | galactitol‑specific enzyme IIB component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 2,176,543 | 0 | C | G | 5.9% | 417.4 / 9.5 | 133 | L53F (TTG→TTC) | gatB | galactitol‑specific enzyme IIB component of PTS |
| Reads supporting (aligned to +/- strand): ref base C (71/54); new base G (2/6); total (73/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 139 total aligned reads displayed.
AATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTA > AP009048/2176474‑2176612 | aaTAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATc < 1:6692379/70‑1 (MQ=255)aaTAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCa < 1:6426851/71‑1 (MQ=255) tAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCaaa < 1:5184509/71‑1 (MQ=255) aGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATGAAATGca > 1:3156233/1‑71 (MQ=255) ggCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGcac < 1:4808873/71‑1 (MQ=255) tGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCaaa > 1:7724404/1‑62 (MQ=255) tGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCaaa > 1:5355323/1‑62 (MQ=255) tGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCaaa > 1:4878905/1‑62 (MQ=255) tGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCaaa > 1:799108/1‑62 (MQ=255) tGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACAcca < 1:3760843/71‑1 (MQ=255) tGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACAcca < 1:2482176/71‑1 (MQ=255) gCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCaaa > 1:267950/1‑61 (MQ=255) gCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCaaa > 1:3934933/1‑61 (MQ=255) accGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCaaa > 1:595701/2‑61 (MQ=255) ccGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATTTGATATGcacca < 1:3085592/67‑3 (MQ=38) ccGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGcacca < 1:3622762/67‑3 (MQ=255) ccGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGcacca < 1:2650792/67‑3 (MQ=255) tGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGTTGCATATCAAATGCACAccatcca > 1:7467022/1‑70 (MQ=255) gAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCa < 1:2147813/54‑1 (MQ=255) gAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACAccat > 1:4666413/1‑66 (MQ=255) gAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACAccat > 1:6913727/1‑66 (MQ=255) aaCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGcaca < 1:3858049/61‑1 (MQ=255) aaCGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCaaa < 1:2364073/51‑1 (MQ=255) aaCGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACAccat < 1:2181395/61‑1 (MQ=255) aCGGAATATCGCCAAAACTACGTTCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGtt > 1:2391208/1‑71 (MQ=255) aCGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGtt > 1:3803061/1‑71 (MQ=255) aCGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGtt > 1:2998293/1‑71 (MQ=255) ggAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTc < 1:1782602/71‑1 (MQ=255) aaTATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATc > 1:4018404/1‑43 (MQ=255) aaTATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATGAAATGCACACCATCCATATAGGTTTCTa > 1:6068937/1‑71 (MQ=255) aaTATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACAccatc > 1:1596878/1‑57 (MQ=255) aaTATCCCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTa > 1:3978454/1‑71 (MQ=255) tatCGCCAAAACTACGATCCACTTTGGCAGTTGTGCATATCAAATGCACACCATCCATATAGGTTTCTAtt < 1:6779555/71‑1 (MQ=255) tatCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAgg < 1:2138118/63‑1 (MQ=255) tatCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTAtt > 1:1403404/1‑71 (MQ=255) atCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTAtt < 1:1396194/70‑1 (MQ=255) tCGCCAAAGCTACGATCCACTTTGGCAGTGGTGCATATCaaa > 1:7219705/1‑42 (MQ=255) tCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCaaa > 1:483277/1‑42 (MQ=255) tCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTAttt > 1:1351470/1‑70 (MQ=255) cGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGcaca > 1:1237747/1‑47 (MQ=255) cGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGcaca > 1:4885847/1‑47 (MQ=255) cGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACAccatcc < 1:728644/53‑1 (MQ=255) cAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCGata < 1:7191104/53‑1 (MQ=255) cAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCata < 1:3880719/53‑1 (MQ=255) aaaaCTTCGATCCACTTTGGCAGTGGTGCATATCa < 1:7738170/35‑1 (MQ=255) aaaaCTACGATCCACTTTGGCAGTGGTTCATATCAAATGCACACCATCCata < 1:3511865/52‑1 (MQ=255) aaaaCTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACAccatcc > 1:1848255/1‑49 (MQ=255) aaaaCTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACAccatcc > 1:580423/1‑49 (MQ=255) aaaaCTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTc > 1:6787230/1‑66 (MQ=255) aaaaCTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTc > 1:7575842/1‑66 (MQ=255) aaaaCTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCAtt < 1:5717274/69‑1 (MQ=255) aaaaCTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCAtt < 1:67450/69‑1 (MQ=255) aaaaCTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCAtt < 1:7373973/69‑1 (MQ=255) aaaaCTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTaa < 1:4645506/71‑1 (MQ=255) aaaaCTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACAACATCCATATAGGTTTCTAt > 1:4558597/1‑63 (MQ=255) aaaCTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTAtt > 1:3479763/1‑63 (MQ=255) aaaCTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTAtt > 1:5189052/1‑63 (MQ=255) aaCTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAAcc > 1:2748553/1‑71 (MQ=255) aCTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAAccc < 1:4869040/71‑1 (MQ=255) aCTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAAccc < 1:6885756/71‑1 (MQ=255) aCTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAAccc < 1:5613456/71‑1 (MQ=255) cTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACAccatcca > 1:1118588/1‑46 (MQ=255) cTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACAccatcca > 1:1199920/1‑46 (MQ=255) cTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACAccatcc > 1:3803036/1‑45 (MQ=255) cGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGaca > 1:6882315/1‑71 (MQ=255) cGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGaca > 1:7430800/1‑71 (MQ=255) cGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGa < 1:6034330/69‑1 (MQ=255) gATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGacac < 1:5936408/71‑1 (MQ=255) tCCACTATGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTg > 1:8088/1‑71 (MQ=255) cACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTgg < 1:2020775/70‑1 (MQ=255) tttGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGAtt > 1:4971179/1‑70 (MQ=255) ttGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCAGACACTGGATTaa > 1:2434684/1‑71 (MQ=255) tGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTaa < 1:3247786/70‑1 (MQ=255) gCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAAtt > 1:2222377/1‑70 (MQ=255) cAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCa < 1:5412531/71‑1 (MQ=255) aGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTaa < 1:6241930/50‑1 (MQ=255) aGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCaa < 1:446637/71‑1 (MQ=255) gtggtgCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCaa > 1:5101605/1‑70 (MQ=255) tggtgCATATCAAATGCACACCATCCATATAGGTTTCTATTTc > 1:2880238/1‑43 (MQ=255) tggtgCATATCAAATGCACACCATCCATATAGGTTTCTATTTc > 1:6532707/1‑43 (MQ=255) tggtgCATATCAAATGCACACCATCCATATAGGTTTCTATTTc > 1:2010849/1‑43 (MQ=255) gtgCATTTGATATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGAtt < 1:2094787/60‑1 (MQ=255) gtgCATATCAAATGCACACCATCCATATAGGTTTCTAtt < 1:1589934/39‑1 (MQ=255) gtgCATATCAAATGCACACCATCCATATAGGTTTCTAtt < 1:5650010/39‑1 (MQ=255) gtgCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAAc > 1:4452723/1‑47 (MQ=255) gtgCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAAc > 1:2274408/1‑47 (MQ=255) tgCATTTGATATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGa < 1:1721400/57‑1 (MQ=255) gCATATCAAATGCACACCATCCATATAGGTTTCTa > 1:5390745/1‑35 (MQ=255) cATATCAAATGCACACCATCCATATAGGTTTCTAttt < 1:3440135/37‑1 (MQ=255) atatCAAATGCACACCATCCATATAGGTTTCTATTTCATTaa < 1:4974375/42‑1 (MQ=255) tatCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGa < 1:7348069/46‑1 (MQ=255) tatCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAatat > 1:1325563/1‑71 (MQ=255) tatCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAata > 1:2562370/1‑70 (MQ=255) tCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGa > 1:5626268/1‑44 (MQ=255) tCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGa > 1:6744966/1‑44 (MQ=255) tCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAAtatta > 1:2664997/1‑71 (MQ=255) tCAAATGCACACCATCCATATAGGTTTATATTTCATTAACCCGa > 1:5252526/1‑44 (MQ=255) cAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCg > 1:5308920/1‑42 (MQ=255) cAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCg > 1:5944918/1‑42 (MQ=255) cAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAAc < 1:1160869/60‑1 (MQ=255) | AATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTA > AP009048/2176474‑2176612 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |