Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AP009048 155,463 A→T 100% *247K (TAA→AAA)  ecpD ← predicted periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP009048155,4630AT100.0% 373.8 / NA 122*247K (TAA→AAA) ecpDpredicted periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (59/63);  total (59/63)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 122 total aligned reads displayed.

TGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAA  >  AP009048/155395‑155530
                                                                    |                                                                   
tgatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCa                                                                   <  1:1193870/71‑1 (MQ=255)
tgatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCa                                                                   <  1:7937633/71‑1 (MQ=255)
tgatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCa                                                                   <  1:5777908/71‑1 (MQ=255)
 gatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAg                                                                  >  1:7057255/1‑71 (MQ=255)
   tgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGcc                                                                >  1:3349396/1‑71 (MQ=255)
   tgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGcc                                                                >  1:6278155/1‑71 (MQ=255)
   tgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGcc                                                                >  1:2106737/1‑71 (MQ=255)
   tgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGcc                                                                >  1:1552139/1‑71 (MQ=255)
   tgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGGGAATTATATCCTTGCCTGATTTCAGCCt                                                               >  1:6365114/1‑71 (MQ=255)
    gatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGcc                                                                <  1:2335573/70‑1 (MQ=255)
     atAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCt                                                               <  1:2467608/70‑1 (MQ=255)
      tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTgg                                                             >  1:5308307/1‑71 (MQ=255)
      tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTgg                                                             <  1:1897435/71‑1 (MQ=255)
       aaTTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTgg                                                             >  1:1407799/1‑70 (MQ=255)
       aaTTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTgg                                                             >  1:7915120/1‑70 (MQ=255)
        aTTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCa                                                           <  1:1090230/71‑1 (MQ=255)
         tttttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCa                                                           <  1:282493/70‑1 (MQ=255)
           tttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAtt                                                         <  1:2455325/70‑1 (MQ=255)
               gTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCtt                                                    <  1:7192277/71‑1 (MQ=255)
                tataTTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTc                                                   <  1:1341657/71‑1 (MQ=255)
                tataTTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTc                                                   <  1:6863589/71‑1 (MQ=255)
                  tatTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCa                                                  <  1:1701480/70‑1 (MQ=255)
                  tatTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCa                                                  <  1:2739183/70‑1 (MQ=255)
                  tatTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCa                                                  <  1:1793174/70‑1 (MQ=255)
                   atTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCt                                                     >  1:894245/1‑66 (MQ=255)
                   atTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAt                                                >  1:5574462/1‑71 (MQ=255)
                     tCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTg                                              >  1:1871658/1‑71 (MQ=255)
                       tataGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAc                                                       >  1:4771823/1‑60 (MQ=255)
                       tataGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAc                                                       >  1:1349918/1‑60 (MQ=255)
                       tataGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgcg                                            >  1:5390811/1‑71 (MQ=255)
                       tataGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgcg                                            >  1:642806/1‑71 (MQ=255)
                         taGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgcgc                                           <  1:4423038/70‑1 (MQ=255)
                           gTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTa                                                        <  1:2518877/55‑1 (MQ=255)
                            tCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAtt                                                         <  1:4573502/53‑1 (MQ=255)
                              aCGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAg                                                                  >  1:4149922/1‑42 (MQ=255)
                              aCGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa                                                 >  1:7459282/1‑59 (MQ=255)
                              aCGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca                                      >  1:744978/1‑70 (MQ=255)
                              aCGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca                                      <  1:5797325/70‑1 (MQ=255)
                              aCGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca                                      <  1:208702/70‑1 (MQ=255)
                               cGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTc                                                   <  1:6201486/56‑1 (MQ=255)
                               cGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTc                                                   <  1:3600006/56‑1 (MQ=255)
                                gCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAg                                   <  1:3173624/71‑1 (MQ=255)
                                gCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAg                                   >  1:7117918/1‑71 (MQ=255)
                                gCTATGCTTCCTGCGGAATTATATCCTTGCCAGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAg                                   >  1:1210026/1‑71 (MQ=255)
                                 cTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCa                                                           >  1:2574213/1‑46 (MQ=255)
                                 cTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCa                                                           >  1:1912154/1‑46 (MQ=255)
                                 cTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt                                  >  1:6820809/1‑71 (MQ=255)
                                    tGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAcc                                                      <  1:2396812/48‑1 (MQ=255)
                                    tGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAt                               <  1:6573000/71‑1 (MQ=255)
                                     gCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAt                               <  1:1283063/70‑1 (MQ=255)
                                     gCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAt                               >  1:7674367/1‑70 (MQ=255)
                                      cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCtt                                                    >  1:2109393/1‑48 (MQ=255)
                                      cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCtt                                                    >  1:2152960/1‑48 (MQ=255)
                                      cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa                                                 >  1:7626424/1‑51 (MQ=255)
                                      cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa                                                 >  1:7701096/1‑51 (MQ=255)
                                      cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca                                      <  1:4528984/62‑1 (MQ=255)
                                      cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAtt                              <  1:1950333/70‑1 (MQ=255)
                                       ttCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAtt                              <  1:430373/69‑1 (MQ=255)
                                        tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTa                             <  1:4523516/69‑1 (MQ=255)
                                         ccTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTaa                            <  1:5904296/69‑1 (MQ=255)
                                          cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCa                                                  <  1:3852917/46‑1 (MQ=255)
                                          cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCa                                                  <  1:7161611/46‑1 (MQ=255)
                                            gCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCg                       >  1:3408058/1‑71 (MQ=255)
                                             cGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAc                                                       >  1:273440/1‑38 (MQ=255)
                                             cGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGt                      <  1:4794536/71‑1 (MQ=255)
                                             cGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGt                      <  1:6828091/71‑1 (MQ=255)
                                               gAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaa                    <  1:5306708/71‑1 (MQ=255)
                                               gAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTa                     >  1:1662555/1‑70 (MQ=255)
                                                aaTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa                                                 <  1:1567684/41‑1 (MQ=255)
                                                aaTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaa                   <  1:5176969/71‑1 (MQ=255)
                                                aaTTATATCCTTGCCGGAATTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaa                    <  1:450387/70‑1 (MQ=255)
                                                 aTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaaa                  <  1:6256957/71‑1 (MQ=255)
                                                 aTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaaa                  <  1:731840/71‑1 (MQ=255)
                                                    atatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgc                                             <  1:1453184/41‑1 (MQ=255)
                                                     tatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAAt                >  1:7698446/1‑69 (MQ=255)
                                                     tatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATg               >  1:4503966/1‑70 (MQ=255)
                                                     tatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGc              <  1:3918684/71‑1 (MQ=255)
                                                     tatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGc              <  1:7283789/71‑1 (MQ=255)
                                                      atCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa                                                 >  1:1186702/1‑35 (MQ=255)
                                                      atCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa                                                 >  1:6183613/1‑35 (MQ=255)
                                                      atCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa                                                 >  1:6192935/1‑35 (MQ=255)
                                                      atCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgcgc                                           >  1:1787386/1‑41 (MQ=255)
                                                      atCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaa                    <  1:5587427/64‑1 (MQ=255)
                                                      atCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCa             >  1:2217828/1‑71 (MQ=255)
                                                       tCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgcgc                                           >  1:3697658/1‑40 (MQ=255)
                                                       tCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgcgc                                           >  1:3147758/1‑40 (MQ=255)
                                                       tCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgcgc                                           >  1:3492795/1‑40 (MQ=255)
                                                         cTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATAATGGCGTAAAAATGCACtt          <  1:7937816/70‑1 (MQ=255)
                                                          ttGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATgg                         >  1:7118067/1‑55 (MQ=255)
                                                           tGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACtttt        >  1:119915/1‑71 (MQ=255)
                                                            gCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACtttt        <  1:2311656/70‑1 (MQ=255)
                                                            gCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACtttt        <  1:6221068/70‑1 (MQ=255)
                                                            gCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACttt         <  1:4900724/69‑1 (MQ=255)
                                                            gCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTg       >  1:7876481/1‑71 (MQ=255)
                                                             ccTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTg       <  1:28610/70‑1 (MQ=255)
                                                             ccTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGc      <  1:3915103/71‑1 (MQ=255)
                                                                gATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATg                          <  1:4757434/48‑1 (MQ=255)
                                                                gATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAg    >  1:6806525/1‑70 (MQ=255)
                                                                 aTTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAg    <  1:4183608/69‑1 (MQ=255)
                                                                 aTTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGaa  <  1:5017875/71‑1 (MQ=255)
                                                                    |                                                                   
TGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAA  >  AP009048/155395‑155530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: