| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 2,946,943 | T→C | 6.2% | I242V (ATC→GTC) | amiC ← | N‑acetylmuramoyl‑L‑alanine amidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 2,946,943 | 0 | T | C | 6.2% | 462.7 / 16.6 | 162 | I242V (ATC→GTC) | amiC | N‑acetylmuramoyl‑L‑alanine amidase |
| Reads supporting (aligned to +/- strand): ref base T (78/74); new base C (5/5); total (83/79) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01 | |||||||||||
Only 100 of 163 total aligned reads displayed.
GATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGC > AP009048/2946875‑2947012 | gATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGt > 1:7070854/1‑71 (MQ=255) aTAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATg > 1:4687213/1‑69 (MQ=255) tAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCt > 1:363227/1‑71 (MQ=255) agagACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGt > 1:5030855/1‑68 (MQ=255) gagaCAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCtt > 1:4113602/1‑70 (MQ=255) aGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGt > 1:396469/1‑71 (MQ=255) aGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGt > 1:6329864/1‑71 (MQ=255) gTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCa < 1:885085/71‑1 (MQ=255) gTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATCTCTTCAtt < 1:3678484/63‑1 (MQ=255) tCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCAtt < 1:4244236/62‑1 (MQ=255) tCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGcgcg < 1:2120383/67‑1 (MQ=255) tCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGcgcg < 1:2530530/67‑1 (MQ=255) cAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTgc > 1:4731231/1‑63 (MQ=255) cAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATg > 1:4019887/1‑71 (MQ=255) aGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATg < 1:5874364/70‑1 (MQ=255) aGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATg > 1:1655163/1‑70 (MQ=255) gCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCa > 1:5504507/1‑57 (MQ=255) cTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCt < 1:7806875/71‑1 (MQ=255) cTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCt < 1:3239745/71‑1 (MQ=255) cTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCt < 1:5154877/71‑1 (MQ=255) tGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCtt > 1:6251323/1‑71 (MQ=255) gTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCtt > 1:7705240/1‑70 (MQ=255) tttCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTc < 1:5868137/70‑1 (MQ=255) tttCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTc < 1:1788476/70‑1 (MQ=255) tttCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCa > 1:4688788/1‑71 (MQ=255) ttCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCtt > 1:924617/1‑46 (MQ=255) ttCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCtt > 1:7066208/1‑46 (MQ=255) ttCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCtt > 1:1372469/1‑46 (MQ=255) ttCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCa > 1:3085061/1‑58 (MQ=255) tCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATg < 1:3556997/41‑1 (MQ=255) tCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATg < 1:296289/41‑1 (MQ=255) ggCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTg < 1:393954/70‑1 (MQ=255) ggCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTg > 1:4780500/1‑70 (MQ=255) ggCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGc > 1:1393913/1‑71 (MQ=255) ttttGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCa > 1:2170854/1‑40 (MQ=255) ttttGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCat < 1:1946143/63‑1 (MQ=255) tttGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCAtt < 1:1802385/41‑1 (MQ=255) tttGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCAtt < 1:4087380/41‑1 (MQ=255) tttGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCAtt < 1:7540263/41‑1 (MQ=255) tttGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCAtt < 1:2077256/41‑1 (MQ=255) tttGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATAtt > 1:1419920/1‑65 (MQ=255) tttGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCctc > 1:925210/1‑71 (MQ=255) tGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTgcgc < 1:1388639/43‑1 (MQ=255) cTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCttt < 1:2922658/70‑1 (MQ=255) cTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATAGCCCTCttt < 1:6168494/70‑1 (MQ=255) aCGCGCACTTGCAATGGAATGAAGATGTCTTCATTgcgc > 1:5600683/1‑39 (MQ=255) aCGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGc < 1:6834015/61‑1 (MQ=255) cgcACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGAt < 1:3234223/71‑1 (MQ=255) gcACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTtctc < 1:1877889/67‑1 (MQ=255) gcACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATc < 1:6532932/71‑1 (MQ=255) cACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCat > 1:7358818/1‑51 (MQ=255) cACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCa < 1:6507623/71‑1 (MQ=255) aCTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGccc > 1:3457132/1‑57 (MQ=255) aCTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGccc > 1:2698513/1‑57 (MQ=255) aCTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGAt > 1:777953/1‑68 (MQ=255) aCTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGAt > 1:5517602/1‑68 (MQ=255) aCTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGAt > 1:1512695/1‑68 (MQ=255) cTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGt > 1:1650113/1‑39 (MQ=255) cTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCa < 1:6222746/69‑1 (MQ=255) ttGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCa > 1:7180538/1‑68 (MQ=255) ttGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCa > 1:4487655/1‑68 (MQ=255) ttGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCa > 1:3192337/1‑68 (MQ=255) ttGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCa > 1:7565462/1‑68 (MQ=255) ttGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCa > 1:5103479/1‑68 (MQ=255) tGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCat < 1:2443690/47‑1 (MQ=255) tGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCat < 1:3136256/47‑1 (MQ=255) gCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCagag < 1:1762078/69‑1 (MQ=255) cAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAgc < 1:2913797/71‑1 (MQ=255) tGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCa < 1:2570590/71‑1 (MQ=255) ggAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCa > 1:4065604/1‑70 (MQ=255) gcaaTGAAGACATCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTtct < 1:2768879/53‑1 (MQ=38) gAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCaaa < 1:5259272/71‑1 (MQ=255) gAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCaaa < 1:5186724/71‑1 (MQ=255) gAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCaa < 1:7628913/70‑1 (MQ=255) aaTGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAAc < 1:7623789/71‑1 (MQ=255) aaTGAAGACATCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCg > 1:1211694/1‑55 (MQ=39) aTGAAGATGTCTTCATTGCGCGTCATGTACACCTTCatat > 1:7438120/1‑40 (MQ=255) aTGAAGACATCTTCATTGCGCGTCATGTACACCTTCATAtt < 1:5405705/41‑1 (MQ=37) aTGAAGACATCTTCATTGCGCGTCATGTACACCTTCATAtt < 1:180057/41‑1 (MQ=37) tGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCagaga > 1:5958207/1‑61 (MQ=255) tGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCagaga > 1:1438045/1‑61 (MQ=255) tGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACgg > 1:7510257/1‑71 (MQ=255) tGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACgg > 1:4273651/1‑71 (MQ=255) tGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACgg < 1:2697772/71‑1 (MQ=255) gAAGATGTCTTCATTGCGCGTCATGTACAGCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGc > 1:2844595/1‑71 (MQ=255) gAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCagaga > 1:1258598/1‑60 (MQ=255) gAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCagaga > 1:7464936/1‑60 (MQ=255) gAAGACATCTTCATTGCGCGTCATGTACACCTTCATATTg > 1:1743867/1‑40 (MQ=37) gAAGACATCTTCATTGCGCGTCATGTACACCTTCATATTGc > 1:2844653/1‑41 (MQ=38) gAAGACATCTTCATTGCGCGTCATGTACACCTTCATATTGc > 1:940505/1‑41 (MQ=38) aaGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAgcg > 1:7455457/1‑62 (MQ=255) aaGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCaaa > 1:4913284/1‑66 (MQ=255) aaGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGc < 1:6482090/70‑1 (MQ=255) aaGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGc < 1:3848191/70‑1 (MQ=255) aaGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGc < 1:2410060/70‑1 (MQ=255) aaGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCg < 1:3855187/71‑1 (MQ=255) gATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCaaa > 1:5004811/1‑64 (MQ=255) aTGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCagaga < 1:5147243/56‑1 (MQ=255) aTGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCagaga < 1:3392247/56‑1 (MQ=255) aTGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCagaga < 1:3375845/56‑1 (MQ=255) | GATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGC > AP009048/2946875‑2947012 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |