| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 2,946,944 | G→A | 6.3% | D241D (GAC→GAT) | amiC ← | N‑acetylmuramoyl‑L‑alanine amidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 2,946,944 | 0 | G | A | 6.3% | 515.4 / 15.5 | 160 | D241D (GAC→GAT) | amiC | N‑acetylmuramoyl‑L‑alanine amidase |
| Reads supporting (aligned to +/- strand): ref base G (78/71); new base A (5/5); total (83/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
Only 100 of 167 total aligned reads displayed.
GATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGC > AP009048/2946875‑2947012 | gATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGt > 1:771815/1‑71 (MQ=255) aTAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATg > 1:4687213/1‑69 (MQ=255) agagACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGt > 1:5030855/1‑68 (MQ=255) gaCAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCAt < 1:745242/71‑1 (MQ=255) aCAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTgcgc > 1:1339633/1‑71 (MQ=255) aCAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTgcgc < 1:5463728/71‑1 (MQ=255) aGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGt > 1:6329864/1‑71 (MQ=255) gTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCa < 1:4215510/71‑1 (MQ=255) gTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCa < 1:885085/71‑1 (MQ=255) gTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATCTCTTCAtt < 1:3678484/63‑1 (MQ=255) tCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCAtt < 1:4244236/62‑1 (MQ=255) tCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGcgcg < 1:2120383/67‑1 (MQ=255) aGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGTAATGAAGATGTCTTCATTGCGCGTCATg < 1:5126200/70‑1 (MQ=255) aGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATg < 1:5874364/70‑1 (MQ=255) aGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATg > 1:1655163/1‑70 (MQ=255) cTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCt < 1:3239745/71‑1 (MQ=255) cTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCt < 1:7806875/71‑1 (MQ=255) cTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCt < 1:5154877/71‑1 (MQ=255) tGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCtt > 1:6251323/1‑71 (MQ=255) gTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCtt > 1:7705240/1‑70 (MQ=255) tttCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTc < 1:1788476/70‑1 (MQ=255) ttCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCtt > 1:7066208/1‑46 (MQ=255) ttCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCa > 1:3085061/1‑58 (MQ=255) tCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATg < 1:3556997/41‑1 (MQ=255) tCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATg < 1:296289/41‑1 (MQ=255) cTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCata < 1:4810583/70‑1 (MQ=255) tGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATg < 1:915798/57‑1 (MQ=255) tGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTa < 1:6814461/59‑1 (MQ=255) gggCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTg > 1:2818235/1‑71 (MQ=255) ggCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTg > 1:4780500/1‑70 (MQ=255) ggCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTg < 1:393954/70‑1 (MQ=255) ggCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTg < 1:1390474/70‑1 (MQ=255) ttttGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCat < 1:2111745/63‑1 (MQ=255) ttttGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCat < 1:1946143/63‑1 (MQ=255) tttGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCctc > 1:7585155/1‑71 (MQ=255) tttGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCctc > 1:925210/1‑71 (MQ=255) ttGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCctct > 1:1785090/1‑71 (MQ=255) tGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTgcgc < 1:1388639/43‑1 (MQ=255) tGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCa > 1:5174684/1‑47 (MQ=255) tGCTACGAGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCa > 1:7525904/1‑47 (MQ=255) cTACGCGCACTTGGAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCttt < 1:242724/70‑1 (MQ=255) cTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCttt < 1:2922658/70‑1 (MQ=255) cTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATAGCCCTCttt < 1:6168494/70‑1 (MQ=255) aCGCGCACTTGCAATGGAATGAAGATGTCTTCATTgcgc > 1:5600683/1‑39 (MQ=255) aCGCGCACTTGCAATGGAATGAAGATGTCTTCATTgcgc > 1:1876456/1‑39 (MQ=255) aCGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGc < 1:5094792/61‑1 (MQ=255) gcgcACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGa < 1:4703887/71‑1 (MQ=255) gcgcACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGa < 1:4031885/71‑1 (MQ=255) cgcACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGAt < 1:3234223/71‑1 (MQ=255) gcACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTtctc < 1:1877889/67‑1 (MQ=255) gcACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATc < 1:6532932/71‑1 (MQ=255) cACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCata > 1:1394959/1‑52 (MQ=255) cACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCat > 1:7358818/1‑51 (MQ=255) cACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCa < 1:6507623/71‑1 (MQ=255) aCTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGccc > 1:3457132/1‑57 (MQ=255) aCTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGccc > 1:2698513/1‑57 (MQ=255) aCTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGAt > 1:3405241/1‑68 (MQ=255) aCTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGAt > 1:5517602/1‑68 (MQ=255) cTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGt > 1:1650113/1‑39 (MQ=255) cTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCa < 1:6222746/69‑1 (MQ=255) ttGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCa > 1:7565462/1‑68 (MQ=255) ttGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCa > 1:3192337/1‑68 (MQ=255) tGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCat < 1:3136256/47‑1 (MQ=255) tGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCat < 1:2443690/47‑1 (MQ=255) tGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCaga > 1:4441014/1‑69 (MQ=255) gcaatgcaatGAAGATGTCTTCATTTCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCagaga < 1:7372228/70‑1 (MQ=255) gCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCagagag > 1:3876816/1‑71 (MQ=255) gCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCagag > 1:1441822/1‑69 (MQ=255) cAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAgc < 1:2913797/71‑1 (MQ=255) tGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCatat < 1:4643698/44‑1 (MQ=255) tGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAgcgc > 1:2323170/1‑70 (MQ=255) ggAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCatat < 1:6907926/43‑1 (MQ=255) ggAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCa > 1:4065604/1‑70 (MQ=255) gcaaTGAAGACATCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTtct < 1:2768879/53‑1 (MQ=38) gAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGAt > 1:397980/1‑58 (MQ=255) gAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCaaa > 1:5861192/1‑71 (MQ=255) gAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCaaa < 1:5259272/71‑1 (MQ=255) gAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCaaa < 1:4959796/71‑1 (MQ=255) gAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCaa < 1:7628913/70‑1 (MQ=255) aaTGAAGACATCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCg > 1:1211694/1‑55 (MQ=39) aTGAAGATGTCTTCATTGCGCGTCATGTACACCTTCatat > 1:2645161/1‑40 (MQ=255) aTGAAGACATCTTCATTGCGCGTCATGTACACCTTCATAtt < 1:7499178/41‑1 (MQ=37) aTGAAGACATCTTCATTGCGCGTCATGTACACCTTCATAtt < 1:5405705/41‑1 (MQ=37) aTGAAGACATCTTCATTGCGCGTCATGTACACCTTCATAtt < 1:180057/41‑1 (MQ=37) tGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCagaga > 1:4092638/1‑61 (MQ=255) tGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCagaga > 1:1438045/1‑61 (MQ=255) tGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCagaga > 1:5958207/1‑61 (MQ=255) gAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCagaga > 1:7464936/1‑60 (MQ=255) gAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCagaga > 1:3661578/1‑60 (MQ=255) gAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCagaga > 1:2538192/1‑60 (MQ=255) gAAGACATCTTCATTGCGCGTCATGTACACCTTCATATTg > 1:1743867/1‑40 (MQ=37) gAAGACATCTTCATTGCGCGTCATGTACACCTTCATATTGc > 1:940505/1‑41 (MQ=38) gAAGACATCTTCATTGCGCGTCATGTACACCTTCATATTGc > 1:2844653/1‑41 (MQ=38) aaGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGc < 1:7077391/70‑1 (MQ=255) aaGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGc < 1:6482090/70‑1 (MQ=255) aaGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGc < 1:3848191/70‑1 (MQ=255) aTGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCagaga < 1:3375845/56‑1 (MQ=255) tGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGc > 1:1559268/1‑70 (MQ=255) gTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCaa > 1:2663677/1‑60 (MQ=255) gTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCaa > 1:1233553/1‑60 (MQ=255) | GATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGC > AP009048/2946875‑2947012 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |