| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 184,000 | G→T | 5.4% | I32I (ATC→ATA) | yaeH ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 184,000 | 0 | G | T | 5.4% | 311.8 / 2.2 | 92 | I32I (ATC→ATA) | yaeH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (39/48); new base T (3/2); total (42/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATA > AP009048/183937‑184069 | cTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCa < 1:5270403/71‑1 (MQ=255) tGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGAAAATCTAtttc > 1:6774857/1‑67 (MQ=255) aCGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAg < 1:4574374/69‑1 (MQ=255) aCGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGaa < 1:5717656/71‑1 (MQ=255) aCGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGaa < 1:5140408/71‑1 (MQ=255) atatTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAg < 1:5482269/63‑1 (MQ=255) atatTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAg < 1:1828061/63‑1 (MQ=255) tatTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgtt < 1:7501758/71‑1 (MQ=255) tatTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgtt < 1:2813205/71‑1 (MQ=255) atTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgttg > 1:6345852/1‑71 (MQ=255) atTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgtt < 1:2302813/70‑1 (MQ=255) tttAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAata < 1:6219053/64‑1 (MQ=255) tttAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgttgt > 1:6766050/1‑71 (MQ=255) tAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGttgttg > 1:7615076/1‑71 (MQ=255) aaaCTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgtt < 1:1994664/66‑1 (MQ=255) aaaCTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGttgttg < 1:6655840/70‑1 (MQ=255) aaaCTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTgg > 1:458432/1‑71 (MQ=255) aaCTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGttgttg < 1:6647019/69‑1 (MQ=255) tAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgttgt > 1:3175211/1‑63 (MQ=255) tAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgtt > 1:2041827/1‑61 (MQ=255) cGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTg > 1:2868358/1‑70 (MQ=255) gCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGcc > 1:3608911/1‑71 (MQ=255) ctctTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATATATTTTCAGAATATCGTTGTTGGCTTCCTGCCg < 1:4273174/71‑1 (MQ=255) ctctTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATATATTTTCAGAATATCGTTGTTGGCTTCCTGCCg < 1:4282350/71‑1 (MQ=255) ctctTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgttgt > 1:788276/1‑58 (MQ=255) tctTGGCGAAAAACTCGCCTTTGTGTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCtt < 1:6239270/63‑1 (MQ=255) tctTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATCTCgttgtt < 1:3569293/58‑1 (MQ=255) tctTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgttgtt < 1:1812157/58‑1 (MQ=255) tctTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCtt < 1:2008621/63‑1 (MQ=255) ttGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCgg < 1:7557905/69‑1 (MQ=255) tGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAgg > 1:7206580/1‑71 (MQ=255) ggCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgtt > 1:3586056/1‑51 (MQ=255) ggCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAgg < 1:246771/70‑1 (MQ=255) ggCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAgg < 1:5074434/70‑1 (MQ=255) ggCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAgg < 1:6511276/70‑1 (MQ=255) gCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCg > 1:6653267/1‑48 (MQ=255) gCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCt < 1:3308386/57‑1 (MQ=255) gCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCt < 1:569425/57‑1 (MQ=255) gCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCt < 1:5192047/57‑1 (MQ=255) gCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCt < 1:2535639/57‑1 (MQ=255) gCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTAAGAatat > 1:5522937/1‑46 (MQ=255) cGAAAAACTCGCCTTTGTCTTTCTTGAAATAGATTTTCAGAATATCGTTGTTGGCTTc < 1:7844670/58‑1 (MQ=255) aaaaCTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGa > 1:1297845/1‑63 (MQ=255) aaaaCTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTaa > 1:7202448/1‑71 (MQ=255) aaaaCTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTaa > 1:1725177/1‑71 (MQ=255) aaaCTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAat > 1:2605201/1‑39 (MQ=255) aaaCTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTaa > 1:180560/1‑70 (MQ=255) aaaCTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAAc < 1:4191521/71‑1 (MQ=255) aaCTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAatat < 1:7419118/40‑1 (MQ=255) aaCTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAAc > 1:2772031/1‑70 (MQ=255) cGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTa > 1:2526412/1‑64 (MQ=255) gCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgttgtt > 1:6495482/1‑42 (MQ=255) gCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATc < 1:1980062/69‑1 (MQ=255) ccTTTGTCTTTCTGGAAATAGATTTTCAGAATATCg < 1:4965971/36‑1 (MQ=255) ccTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAAc < 1:4224566/64‑1 (MQ=255) cTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAAtt < 1:7835443/71‑1 (MQ=255) cTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAAtt < 1:431469/71‑1 (MQ=255) cTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAAtt < 1:4029184/71‑1 (MQ=255) cTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAAtt < 1:1739079/71‑1 (MQ=255) cTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAAtt < 1:7580792/71‑1 (MQ=255) cTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAAt < 1:2083558/70‑1 (MQ=255) tttGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTcc > 1:4489160/1‑46 (MQ=255) tttGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTcc > 1:3396349/1‑46 (MQ=255) ttGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATttc > 1:5429720/1‑71 (MQ=255) ttGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATttc > 1:3172639/1‑71 (MQ=255) tGTCTTTCTGGAAATAGATTTTCAGAATATCgttg > 1:5646374/1‑35 (MQ=255) tGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCt > 1:1694662/1‑45 (MQ=255) tGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCt > 1:4276149/1‑45 (MQ=255) tGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCt > 1:3355567/1‑45 (MQ=255) tGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAgg > 1:3732304/1‑53 (MQ=255) tattCTGAAAATCTATTTCCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATttctt > 1:648375/3‑69 (MQ=37) tttCTGGAAATAGATTTTCAGAATATCGTTGTTGGCtt < 1:6636238/38‑1 (MQ=255) tCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAgg < 1:7879826/70‑1 (MQ=255) tCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGa > 1:3950032/1‑71 (MQ=255) tCTGAAAATCTATTTCCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGa > 1:647666/1‑71 (MQ=38) ggAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTg > 1:3833548/1‑71 (MQ=255) gAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGAt < 1:5735807/68‑1 (MQ=255) gAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGAt < 1:3300254/68‑1 (MQ=255) gAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTgg < 1:3799796/71‑1 (MQ=255) gAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTgg < 1:1733249/71‑1 (MQ=255) aaaTAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGt < 1:3795247/71‑1 (MQ=255) aTAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTaa < 1:1951533/71‑1 (MQ=255) aTAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTaa < 1:3114838/71‑1 (MQ=255) aTAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTaa < 1:90862/71‑1 (MQ=255) tAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAg > 1:6241821/1‑38 (MQ=255) tAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATttctt > 1:545033/1‑58 (MQ=255) tAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCa > 1:2321477/1‑60 (MQ=255) tAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCa > 1:6803469/1‑60 (MQ=255) tAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAAt < 1:4370124/71‑1 (MQ=255) aGATTTTCAGAATATCGTTGTTGGCTTCCTGCCgg > 1:2299264/1‑35 (MQ=255) aGATTTTCAGAATATCGTTGTTGGCTTCCTGCCgg > 1:4548747/1‑35 (MQ=255) aGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTaa > 1:3446807/1‑44 (MQ=255) gATTTTCAGAATATCGTTGTTGGCTTCCTGCCGTAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATa < 1:7479612/70‑1 (MQ=255) gATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGAtt > 1:3587770/1‑63 (MQ=255) gATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATa < 1:4463700/70‑1 (MQ=255) | CTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATA > AP009048/183937‑184069 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |