| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,222,510 | G→A | 6.0% | V408I (GTC→ATC) | ebgA → | cryptic beta‑D‑galactosidase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,222,510 | 0 | G | A | 6.0% | 432.6 / 11.1 | 133 | V408I (GTC→ATC) | ebgA | cryptic beta‑D‑galactosidase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (59/66); new base A (4/4); total (63/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
Only 100 of 133 total aligned reads displayed.
AACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTT > AP009048/3222442‑3222575 | aaCTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTc < 1:469384/71‑1 (MQ=255) aCTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCg > 1:2968538/1‑71 (MQ=255) aCTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCg < 1:2148082/71‑1 (MQ=255) aCTGTGTGATATCTACGGCCTATTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCg < 1:7507135/71‑1 (MQ=255) tgtgtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGc > 1:2399760/1‑71 (MQ=255) tgtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGc < 1:1865307/69‑1 (MQ=255) tgtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGa < 1:2110493/71‑1 (MQ=255) gtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGc < 1:3496776/68‑1 (MQ=255) gATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGa > 1:4354423/1‑68 (MQ=255) gATATCTACGACCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGa > 1:6284936/1‑68 (MQ=255) atatCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGatat > 1:1720339/1‑70 (MQ=255) atatCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATAtt < 1:2884763/71‑1 (MQ=255) tatCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGatat > 1:3923442/1‑69 (MQ=255) tatCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTa < 1:6407565/71‑1 (MQ=255) tatCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTa < 1:154249/71‑1 (MQ=255) tACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGata > 1:6177278/1‑64 (MQ=255) tACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCg > 1:4811664/1‑71 (MQ=255) cGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGcc < 1:6391694/68‑1 (MQ=255) cGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCg < 1:2121619/69‑1 (MQ=255) gCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTAtt < 1:5394092/71‑1 (MQ=255) ccTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATATCGCCGACAtt < 1:5182558/61‑1 (MQ=38) ccTGTTTGTGATGGCGGAAACCGAAGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTa > 1:4211744/1‑71 (MQ=255) tGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTAcc > 1:135184/1‑71 (MQ=255) gTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGcc < 1:1693253/62‑1 (MQ=255) gTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGc < 1:6437200/61‑1 (MQ=255) gTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTAc > 1:5787070/1‑69 (MQ=255) tttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCg < 1:7161311/49‑1 (MQ=255) tttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCg < 1:5997816/49‑1 (MQ=255) tttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCg < 1:1954035/49‑1 (MQ=255) tttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCg < 1:1941551/49‑1 (MQ=255) tttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCg < 1:7918059/49‑1 (MQ=255) tttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGat < 1:4186610/54‑1 (MQ=255) tttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTAcc < 1:2884511/69‑1 (MQ=255) tttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTAcc < 1:2382873/69‑1 (MQ=255) ttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcgac > 1:5561100/1‑71 (MQ=255) ttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcgac > 1:3576333/1‑71 (MQ=255) tgtgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcgac > 1:6492658/1‑70 (MQ=255) tgtgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcgac > 1:2656534/1‑70 (MQ=255) gtgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcgacga > 1:3179019/1‑71 (MQ=255) gtgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcgacg < 1:4163481/70‑1 (MQ=255) gtgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcga > 1:3785725/1‑68 (MQ=255) tgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcgacga < 1:5858276/70‑1 (MQ=255) gATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCgg < 1:1239818/45‑1 (MQ=255) gATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCgg < 1:6101283/45‑1 (MQ=255) gATGGCGGAAACCGACGGCGAATCGCACGGCTTTGCTAATGTCgg < 1:7895175/45‑1 (MQ=255) aTGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATcc > 1:1119823/1‑71 (MQ=255) aTGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATcc > 1:7249234/1‑71 (MQ=255) aTGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATcc > 1:4637556/1‑71 (MQ=255) aTGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATc < 1:3559074/70‑1 (MQ=255) aTGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATc < 1:4751617/70‑1 (MQ=255) tGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATAtt < 1:7842407/50‑1 (MQ=255) tGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCg > 1:6948956/1‑71 (MQ=255) ggCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCg > 1:900684/1‑70 (MQ=255) gCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGt < 1:4804947/54‑1 (MQ=255) gCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGc < 1:228498/70‑1 (MQ=255) gCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGT‑GGCGATATTAGCCGTATTACCGACGATCCGCa > 1:5630731/1‑70 (MQ=255) cGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCa > 1:862584/1‑70 (MQ=255) cGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAg < 1:7692023/71‑1 (MQ=255) cGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAg < 1:5132735/71‑1 (MQ=255) ggAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGt > 1:6352803/1‑71 (MQ=255) ggAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGt > 1:6420729/1‑71 (MQ=255) ggAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGt > 1:919776/1‑71 (MQ=255) gAAACCGACGTCGAATCGCACGGCTTTGCTCATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTg < 1:7741713/71‑1 (MQ=255) aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCgg > 1:6821925/1‑37 (MQ=255) aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCgg > 1:7216908/1‑37 (MQ=255) aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGata > 1:3658354/1‑42 (MQ=255) aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGata > 1:307642/1‑42 (MQ=255) aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTAtt > 1:3521597/1‑53 (MQ=255) aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcga > 1:6674146/1‑58 (MQ=255) cGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGaaa < 1:5888691/71‑1 (MQ=255) aCGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGaaa > 1:5875574/1‑69 (MQ=255) aCGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGaaa > 1:1267208/1‑69 (MQ=255) aCGTCGAATCGCACGGCTTTGCTAATATCGCCGacat > 1:4893642/1‑37 (MQ=21) aCGTCGAATCGCACGGCTTTGCTAATATCGCCGacat > 1:6535172/1‑37 (MQ=21) gTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTAcc > 1:2354259/1‑48 (MQ=255) gTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGt < 1:7048147/71‑1 (MQ=255) gTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGt > 1:2414969/1‑71 (MQ=255) tCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGc < 1:2508183/57‑1 (MQ=255) tCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTc < 1:1931433/71‑1 (MQ=255) tCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTc < 1:1175409/71‑1 (MQ=255) gAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGt > 1:7204700/1‑39 (MQ=255) gAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGt > 1:5113229/1‑39 (MQ=255) gAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGt > 1:536200/1‑39 (MQ=255) gAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGt > 1:4023159/1‑39 (MQ=255) gAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAg < 1:4487293/67‑1 (MQ=255) gAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAg < 1:2305156/67‑1 (MQ=255) aaTCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAg < 1:2295913/66‑1 (MQ=255) gCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGt > 1:4269112/1‑53 (MQ=255) gCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAgcgc < 1:2559231/71‑1 (MQ=255) cTTTGCTAATGTCGGCGATATTAGACGTATTACCGACGATCCGCAGTGGGAAAAAGTCTAAGTCGAgcg > 1:7467087/1‑69 (MQ=255) ttGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCAtt > 1:2485572/1‑71 (MQ=255) tGCTAATGTCGGCGATATTAGCCGTATTACcgacga < 1:7473972/36‑1 (MQ=255) cTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGaaa < 1:3415549/48‑1 (MQ=255) cTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGaaa < 1:1208167/48‑1 (MQ=255) cTAATATCGCCGACATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGtt > 1:4260188/1‑71 (MQ=39) aTGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCt > 1:6229095/1‑51 (MQ=255) aTGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACg > 1:2775568/1‑54 (MQ=255) aTGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACg > 1:3144124/1‑54 (MQ=255) aTGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGt < 1:753140/67‑1 (MQ=255) tGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGa < 1:7601017/57‑1 (MQ=255) | AACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTT > AP009048/3222442‑3222575 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |