| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,222,514 | G→C | 6.3% | G409A (GGC→GCC) | ebgA → | cryptic beta‑D‑galactosidase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,222,514 | 0 | G | C | 6.3% | 390.4 / 10.5 | 126 | G409A (GGC→GCC) | ebgA | cryptic beta‑D‑galactosidase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (58/60); new base C (4/4); total (62/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01 | |||||||||||
Only 100 of 143 total aligned reads displayed.
TGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATC > AP009048/3222445‑3222584 | tgtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGa < 1:2110493/71‑1 (MQ=255) gtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGc < 1:3496776/68‑1 (MQ=255) gtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGa > 1:2014113/1‑70 (MQ=255) gATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGa > 1:4354423/1‑68 (MQ=255) atatCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGatat > 1:1720339/1‑70 (MQ=255) atatCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATAtt < 1:2884763/71‑1 (MQ=255) tatCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGatat > 1:3923442/1‑69 (MQ=255) tatCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTa < 1:154249/71‑1 (MQ=255) tatCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTa < 1:6407565/71‑1 (MQ=255) tCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGc > 1:1919479/1‑71 (MQ=255) tACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGata > 1:6177278/1‑64 (MQ=255) ggCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCg < 1:2161734/68‑1 (MQ=255) ccTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATATCGCCGACAtt < 1:5182558/61‑1 (MQ=38) ccTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATATCGCCGACAtt < 1:5405375/61‑1 (MQ=38) ccTGTTTGTGATGGCGGAAACCGAAGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTa > 1:4211744/1‑71 (MQ=255) tGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTAcc > 1:135184/1‑71 (MQ=255) ggttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGa < 1:286493/52‑1 (MQ=255) gTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGcc < 1:1693253/62‑1 (MQ=255) gTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTAc > 1:5787070/1‑69 (MQ=255) tttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGat < 1:4186610/54‑1 (MQ=255) tttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGt < 1:4814421/63‑1 (MQ=255) tttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGt < 1:5840153/63‑1 (MQ=255) tttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTAcc < 1:2382873/69‑1 (MQ=255) tttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTAcc < 1:2884511/69‑1 (MQ=255) ttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcgac > 1:3576333/1‑71 (MQ=255) ttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcgac > 1:5561100/1‑71 (MQ=255) tgtgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcgac > 1:2656534/1‑70 (MQ=255) tgtgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcgac > 1:6492658/1‑70 (MQ=255) gtgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcgacg < 1:4163481/70‑1 (MQ=255) gtgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcga > 1:3785725/1‑68 (MQ=255) gATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCgg < 1:6101283/45‑1 (MQ=255) gATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCgg < 1:1239818/45‑1 (MQ=255) aTGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATcc > 1:7249234/1‑71 (MQ=255) aTGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATcc > 1:1119823/1‑71 (MQ=255) aTGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATcc > 1:4637556/1‑71 (MQ=255) aTGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATc < 1:4751617/70‑1 (MQ=255) tGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATAtt < 1:7842407/50‑1 (MQ=255) ggCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCg > 1:900684/1‑70 (MQ=255) gCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGt < 1:4804947/54‑1 (MQ=255) gCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcgac > 1:697503/1‑63 (MQ=255) cGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAg < 1:6373049/71‑1 (MQ=255) cGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAg < 1:5132735/71‑1 (MQ=255) cGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAg < 1:7692023/71‑1 (MQ=255) ggAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTAcc > 1:2537456/1‑58 (MQ=255) ggAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGt > 1:6420729/1‑71 (MQ=255) ggAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGt > 1:6352803/1‑71 (MQ=255) ggAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGt > 1:919776/1‑71 (MQ=255) aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCgg > 1:2402557/1‑37 (MQ=255) aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCgg > 1:7216908/1‑37 (MQ=255) aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCgg > 1:6821925/1‑37 (MQ=255) aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGata > 1:3658354/1‑42 (MQ=255) aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGata > 1:307642/1‑42 (MQ=255) aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcga > 1:6674146/1‑58 (MQ=255) cGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGaaa < 1:6428472/71‑1 (MQ=255) cGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGaaa < 1:5888691/71‑1 (MQ=255) aCGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGaaa > 1:1267208/1‑69 (MQ=255) aCGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGaaa > 1:5875574/1‑69 (MQ=255) aCGTCGAATCGCACGGCTTTGCTAATATCGCCGacat > 1:6535172/1‑37 (MQ=21) aCGTCGAATCGCACGGCTTTGCTAATATCGCCGacat > 1:4893642/1‑37 (MQ=21) gTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTAcc > 1:2354259/1‑48 (MQ=255) gTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGt < 1:7048147/71‑1 (MQ=255) gTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGt > 1:2414969/1‑71 (MQ=255) tCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCg < 1:861106/56‑1 (MQ=255) tCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTc < 1:7580434/71‑1 (MQ=255) gAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGt > 1:536200/1‑39 (MQ=255) gAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGt > 1:4023159/1‑39 (MQ=255) gAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGt > 1:5113229/1‑39 (MQ=255) gAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGt > 1:7204700/1‑39 (MQ=255) gAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAg < 1:2305156/67‑1 (MQ=255) gAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAg < 1:4487293/67‑1 (MQ=255) aaTCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAg < 1:2295913/66‑1 (MQ=255) tCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGt > 1:7772111/1‑71 (MQ=255) gCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGt > 1:4269112/1‑53 (MQ=255) gCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAgcgc < 1:2559231/71‑1 (MQ=255) ttGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCAtt > 1:2485572/1‑71 (MQ=255) tGCTAATGTCGGCGATATTAGCCGTATTACcgacga < 1:7473972/36‑1 (MQ=255) cTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGaaa < 1:1208167/48‑1 (MQ=255) cTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGaaa < 1:3415549/48‑1 (MQ=255) cTAATATCGCCGACATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGtt > 1:4260188/1‑71 (MQ=39) cTAATATCGCCGACATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGt > 1:3546129/1‑70 (MQ=39) aTGTCGGCGATATTAGCCGTATTACCGACGATCCGCa < 1:7468206/37‑1 (MQ=255) aTGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCt > 1:6229095/1‑51 (MQ=255) aTGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACg > 1:2775568/1‑54 (MQ=255) aTGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACg > 1:3144124/1‑54 (MQ=255) tGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGa < 1:7601017/57‑1 (MQ=255) tCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCg < 1:5103454/54‑1 (MQ=255) tCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAgc > 1:2259548/1‑57 (MQ=255) tCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAgc > 1:423104/1‑57 (MQ=255) tCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGcc > 1:1031881/1‑69 (MQ=255) cGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAgcg < 1:2259330/57‑1 (MQ=255) cGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAgcg < 1:1892721/57‑1 (MQ=255) cGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAgcg < 1:1082703/57‑1 (MQ=255) ggCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTAc > 1:3558813/1‑48 (MQ=255) ggCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTAc > 1:6158217/1‑48 (MQ=255) ggCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTg < 1:3210260/61‑1 (MQ=255) gCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTa > 1:3266410/1‑46 (MQ=255) gCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTa > 1:806448/1‑46 (MQ=255) gCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTa > 1:3452776/1‑46 (MQ=255) gCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTa > 1:6249435/1‑46 (MQ=255) gCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCa > 1:5493106/1‑57 (MQ=255) | TGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATC > AP009048/3222445‑3222584 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |