| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,651,710 | T→G | 5.0% | L346R (CTT→CGT) | aslA → | acrylsulfatase‑like enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,651,710 | 0 | T | G | 5.0% | 324.9 / 10.9 | 119 | L346R (CTT→CGT) | aslA | acrylsulfatase‑like enzyme |
| Reads supporting (aligned to +/- strand): ref base T (47/66); new base G (3/3); total (50/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01 | |||||||||||
Only 100 of 128 total aligned reads displayed.
GCGACTGCATGGTGGAGATGAACGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTACCGCCGCACGGACGCACC > AP009048/3651641‑3651780 | gCGACTGCATGGTGGAGATGAACGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCtt > 1:5258987/1‑71 (MQ=255)gCGACTGCATGGTGGAGATGAACGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCt < 1:863631/70‑1 (MQ=255) gACTGCATGGTGGAGATGAACGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTg > 1:6610982/1‑70 (MQ=255) gACTGCATGGTGGAGATGAACGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGATCAGCGTgt > 1:6508219/1‑70 (MQ=255) gACTGCATGGTGGAGATGAACGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGATCAGCGTgt > 1:5962358/1‑70 (MQ=255) aCTGCATGGTGGAGATGAACGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGa > 1:6371861/1‑70 (MQ=255) aTGGTGGAGATGAACGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAacac > 1:3828792/1‑71 (MQ=255) tggtggAGATGAACGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACg > 1:6098614/1‑71 (MQ=255) ggtggAGATGAACGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGc > 1:3420945/1‑71 (MQ=255) gtggAGATGAACGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCt > 1:1583546/1‑71 (MQ=255) agaTGAACGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATc < 1:1541950/71‑1 (MQ=255) aTGAACGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGt < 1:7528742/71‑1 (MQ=255) tGAACGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCtt < 1:3288355/53‑1 (MQ=255) tGAACGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTc < 1:1769149/71‑1 (MQ=255) tGAACGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTc < 1:7732248/71‑1 (MQ=255) gAACGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCt > 1:303139/1‑71 (MQ=255) aaCGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGATCAGCGTGTTATCAAGCTGACCGt < 1:7399474/68‑1 (MQ=21) aCGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCg < 1:4760911/66‑1 (MQ=255) aCGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCtt > 1:2092084/1‑70 (MQ=255) aCGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCtt > 1:3550573/1‑70 (MQ=255) cGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAGACGATCAGCGTGTTATCAAGCTGACCGTttttc < 1:4079353/71‑2 (MQ=14) tgtTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTcc > 1:7518265/1‑71 (MQ=255) gtTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTcc > 1:7003700/1‑70 (MQ=255) ttCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCg < 1:1920022/70‑1 (MQ=255) ttCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCg < 1:4786007/70‑1 (MQ=255) tCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCtt > 1:3097622/1‑41 (MQ=255) cGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGAt < 1:3919313/70‑1 (MQ=255) cGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGAt < 1:3854636/70‑1 (MQ=255) gCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTg < 1:4957072/40‑1 (MQ=255) gCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATa < 1:2082236/70‑1 (MQ=255) cTAATCTGTATAAAACACTGTAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAAc < 1:7900438/71‑1 (MQ=255) cTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTg < 1:7693941/50‑1 (MQ=255) cTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTg < 1:3323130/50‑1 (MQ=255) cTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTg < 1:2631101/50‑1 (MQ=255) tAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACg > 1:6487893/1‑71 (MQ=255) aaaTCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAAc > 1:3785644/2‑70 (MQ=255) aTCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACg > 1:3946687/1‑44 (MQ=255) aTCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACgg < 1:7511200/70‑1 (MQ=255) aTCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACgg < 1:7935701/70‑1 (MQ=255) aTCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACg < 1:3373436/69‑1 (MQ=255) aTCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGa > 1:6083749/1‑71 (MQ=255) aTCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGa < 1:4624653/71‑1 (MQ=255) tCTTTATAAAACACTGGAGAAAAACGGTCAGCTTGATAacac < 1:7188341/42‑1 (MQ=255) gTATAAAACACTGGAGAAAAACGGTCAGCTTGATAacac < 1:1498879/39‑1 (MQ=255) gTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTg < 1:3165267/43‑1 (MQ=255) tataAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGAt < 1:6840111/60‑1 (MQ=255) tataAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGAcc < 1:3672755/68‑1 (MQ=255) tataAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGa < 1:2714119/71‑1 (MQ=255) taAAACACTGGAGAAAAACGGTCAGCTTGATAacac < 1:4393311/36‑1 (MQ=255) taAAACACTGGAGAAAAACGGTCAGCTTGATAacac < 1:4973333/36‑1 (MQ=255) taAAACACTGGAGAAAAACGGTCAGCTTGATAacac < 1:3143662/36‑1 (MQ=255) taAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAg > 1:7624872/1‑71 (MQ=255) taAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAg > 1:6687869/1‑71 (MQ=255) aaaCACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATa > 1:6553007/1‑57 (MQ=255) aaaCACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATa > 1:3016634/1‑57 (MQ=255) acacTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCg > 1:5065768/1‑70 (MQ=255) cacTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAAc > 1:3957718/1‑56 (MQ=255) cacTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCg > 1:2239563/1‑69 (MQ=255) cacTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCg > 1:3210902/1‑69 (MQ=255) cacTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCg > 1:2617916/1‑69 (MQ=255) cacTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGaa < 1:5155610/71‑1 (MQ=255) acTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCt < 1:6897878/41‑1 (MQ=255) gagaAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTAcc < 1:6173282/71‑1 (MQ=255) gagaAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTAc < 1:1066787/70‑1 (MQ=255) gaAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTAccgc > 1:7186458/1‑71 (MQ=255) aaaaaCGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCg < 1:5680289/43‑1 (MQ=255) aaaaaCGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCg < 1:5846948/43‑1 (MQ=255) aaaaaCGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCg < 1:6000524/43‑1 (MQ=255) aaaaaCGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCg < 1:4247402/43‑1 (MQ=255) aaaaaCGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGa > 1:563713/1‑44 (MQ=255) aaaaaCGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGa > 1:5704726/1‑44 (MQ=255) aaaaCGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTAccgccg < 1:7693055/71‑1 (MQ=255) aaaaCGGGCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGa < 1:5844962/50‑1 (MQ=255) aaaCGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGaa > 1:4940237/1‑55 (MQ=255) aaaCGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGaa > 1:2747732/1‑55 (MQ=255) aaaCGGTCAGCTTGATAACACGCTGATCGTCTTCACCTCCGATAACGGACCGGAAGCCGAAGTAccgccg > 1:1682535/1‑70 (MQ=255) aCGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTACCGCCGCAc > 1:4719627/1‑71 (MQ=255) aCGATCAGCGTGTTATCAAGCTGACCGTTTTTACCTCCGATAACGGACCGGAAGCCGAAGTACCGCCGCAc > 1:4769844/1‑71 (MQ=18) ggTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGt < 1:6333968/59‑1 (MQ=255) ggTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGt < 1:1829372/59‑1 (MQ=255) ggTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGt < 1:2585041/59‑1 (MQ=255) ggTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTACCGCCGCACgg < 1:1412165/71‑1 (MQ=255) ggTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTACCGCCGCACgg < 1:3269563/71‑1 (MQ=255) ggTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTACCGCAGCACgg < 1:2022395/71‑1 (MQ=255) gTCAGCTTGATAACACGCTGATCGTCTTTACCTcc < 1:2459520/35‑1 (MQ=255) gTCAGCTTGATAACACGCTGATCGTCTTTACCTcc < 1:3285592/35‑1 (MQ=255) gTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTACCGCCGCACGGa < 1:4480103/71‑1 (MQ=255) gTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTACCGCCGCACGGa > 1:5381695/1‑71 (MQ=255) gTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTACCGCCGCACGGa > 1:5372847/1‑71 (MQ=255) tCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTACCGCCGCACg > 1:2873539/1‑68 (MQ=255) aGCGTGTTATCAAGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTACCGCCGCACGGACg < 1:2140792/70‑1 (MQ=38) cTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTAccg > 1:6684135/1‑57 (MQ=255) cTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTAccg > 1:3092069/1‑57 (MQ=255) cTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTAccg > 1:1183052/1‑57 (MQ=255) cTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTACCGCCGCACGGa > 1:1553818/1‑66 (MQ=255) ttGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGa < 1:6796697/43‑1 (MQ=255) ttGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGa < 1:2264386/43‑1 (MQ=255) ttGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAg < 1:4558498/51‑1 (MQ=255) ttGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAg < 1:3346516/51‑1 (MQ=255) ttGATAACACGCTGATCGTCTTTACATCCGATAACGGACCGGAAGCCGAAGTACCGCCGCACGGACGCAcc > 1:1328946/1‑71 (MQ=255) | GCGACTGCATGGTGGAGATGAACGATGTGTTCGCTAATCTGTATAAAACACTGGAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAAGCCGAAGTACCGCCGCACGGACGCACC > AP009048/3651641‑3651780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |