| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,684,513 | G→A | 100% | pseudogene (546/582 nt) pseudogene (1609/1645 nt) |
ilvG ← ilvG ← |
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,684,513 | 0 | G | A | 100.0% | 232.0 / NA | 79 | pseudogene (546/582 nt) pseudogene (1609/1645 nt) | ilvG ilvG | ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (46/33); total (46/33) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAGCGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCGCCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTATGGCC > AP009048/3684446‑3684577 | aaGCGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCAcc < 1:1160132/70‑1 (MQ=255) gAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAggc < 1:7071763/70‑1 (MQ=255) cGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCa < 1:5169527/70‑1 (MQ=255) cATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGc < 1:2926422/70‑1 (MQ=255) cATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCg > 1:5774484/1‑71 (MQ=255) tGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCgg > 1:3704123/1‑69 (MQ=255) tGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGc > 1:5460095/1‑70 (MQ=255) gACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGcc < 1:3158713/70‑1 (MQ=255) aCCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAg < 1:155761/71‑1 (MQ=255) aCCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAg < 1:6572883/71‑1 (MQ=255) aTGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAggcgg > 1:6727837/1‑53 (MQ=255) aTGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAggcgg > 1:6314412/1‑53 (MQ=255) aTGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAggcg > 1:6889009/1‑52 (MQ=255) aTGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACg < 1:1740452/69‑1 (MQ=255) aTGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACg < 1:4032080/69‑1 (MQ=255) cctGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTtc < 1:4518341/68‑1 (MQ=255) ttGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTtctc < 1:693548/71‑1 (MQ=255) tGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTtctc > 1:4557881/1‑70 (MQ=255) agCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAgg > 1:6722924/2‑46 (MQ=255) gCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTtctc > 1:5560162/1‑69 (MQ=255) atcatGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGt > 1:6408390/1‑71 (MQ=255) atGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAg > 1:7070442/1‑49 (MQ=255) atGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtcg > 1:947764/1‑71 (MQ=255) atGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtcg < 1:810439/71‑1 (MQ=255) atGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtcg > 1:3278209/1‑71 (MQ=255) tGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCAc > 1:7800941/1‑45 (MQ=255) gATAATTTCTCCAACATTTCTGAATTACTGGCACCa < 1:3160647/36‑1 (MQ=255) gATAATTTCTCCAACATTTCTGAATTACTGGCACCa < 1:3468738/36‑1 (MQ=255) gATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGc > 1:4832708/1‑51 (MQ=255) gATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtcgtc > 1:571513/1‑71 (MQ=255) tAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGc > 1:253868/1‑49 (MQ=255) tAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGa > 1:2397009/1‑71 (MQ=255) tAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGa > 1:6213792/1‑71 (MQ=255) tAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGa < 1:3049236/71‑1 (MQ=255) tAATTTATCCAACATTTCTGAATTACTGGCACCAGGCGGCACCa > 1:1269326/1‑44 (MQ=38) aTTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTATCAAGTTCGTCGAtt < 1:734556/71‑1 (MQ=255) ttCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCa > 1:719838/1‑40 (MQ=255) ttCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAtt > 1:1246229/1‑69 (MQ=255) ttCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAtt > 1:3064703/1‑69 (MQ=255) tctcCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCCAGTTCGTCGATTga < 1:7382209/70‑1 (MQ=255) tctcCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAg > 1:4653672/1‑58 (MQ=255) tctcCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAtt > 1:1941082/1‑68 (MQ=255) tcCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtc < 1:4502611/59‑1 (MQ=255) cAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTg > 1:1641899/1‑65 (MQ=255) aaCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCTGACGTTCTCAAGTTCGTCGATTGAGACa < 1:6548967/69‑1 (MQ=255) aaCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACAt > 1:1505294/1‑70 (MQ=255) aaCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATg < 1:7387432/71‑1 (MQ=255) aCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAtt < 1:416661/62‑1 (MQ=255) aCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAtt < 1:2414902/62‑1 (MQ=255) aCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGa < 1:7659968/71‑1 (MQ=255) tttCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGa > 1:615101/1‑39 (MQ=255) tttCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGa > 1:2046072/1‑39 (MQ=255) tttCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCa > 1:4008807/1‑47 (MQ=255) tttCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCa > 1:4621742/1‑47 (MQ=255) tttCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGc < 1:2603837/71‑1 (MQ=255) tttCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGc < 1:1546348/71‑1 (MQ=255) tttCTGAATTACTGGCACAAGGCGGCACCAGCGGCCAGACGTTCTCa > 1:2743688/1‑47 (MQ=38) ttCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtcgtcg > 1:3293776/1‑55 (MQ=255) ttCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAg > 1:3892601/1‑69 (MQ=255) tCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAg > 1:3772445/1‑71 (MQ=255) cTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAg < 1:1245624/70‑1 (MQ=255) cTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAg < 1:694520/70‑1 (MQ=255) ttaaTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTg < 1:7099572/54‑1 (MQ=255) tGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTtct > 1:2725764/1‑41 (MQ=255) tGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTg < 1:405798/56‑1 (MQ=255) aaTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTtctc > 1:6220930/1‑40 (MQ=255) aaTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTtctc > 1:7301263/1‑40 (MQ=255) aaTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTtctc > 1:7192636/1‑40 (MQ=255) aaTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAgg < 1:1491826/68‑1 (MQ=255) aaTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTAt > 1:1350206/1‑71 (MQ=255) aaTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTAt > 1:6794438/1‑71 (MQ=255) aTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTATg > 1:88795/1‑71 (MQ=255) ttACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAtt < 1:3378270/51‑1 (MQ=255) ttACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTATgg > 1:2942319/1‑71 (MQ=255) aCTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGa > 1:444585/1‑58 (MQ=255) aCTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGa > 1:834669/1‑58 (MQ=255) aCTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTATGGcc > 1:5405582/1‑71 (MQ=255) tGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTATGGcc < 1:5345161/69‑1 (MQ=255) ggCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGc < 1:5066596/58‑1 (MQ=255) | AAGCGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCGCCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTATGGCC > AP009048/3684446‑3684577 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |