Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AP009048 3,684,705 G→A 100% pseudogene (354/582 nt)
pseudogene (1417/1645 nt)
ilvG ←
ilvG ←
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0090483,684,7050GA100.0% 399.3 / NA 132pseudogene (354/582 nt)
pseudogene (1417/1645 nt)
ilvG
ilvG
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (67/65);  total (67/65)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 136 total aligned reads displayed.

GGCCATGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCAGTAAGGGTGGTTTCGCTGTATCGTTCCTGAAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGA  >  AP009048/3684638‑3684772
                                                                   |                                                                   
  ccAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCtata                                                                   <  1:2548426/68‑1 (MQ=255)
   cagggATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCAGTAAGGGTGGTTTCGCTATATCGt                                                               <  1:2471108/68‑1 (MQ=255)
    agggATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGtt                                                              <  1:1589162/69‑1 (MQ=255)
     gggATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTc                                                             <  1:2930424/70‑1 (MQ=255)
     gggATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTc                                                             <  1:1579980/70‑1 (MQ=255)
        aTGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTg                                                          <  1:3614566/71‑1 (MQ=255)
        aTGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTg                                                          >  1:4634083/1‑71 (MQ=255)
         tGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTg                                                          >  1:7610253/1‑70 (MQ=255)
           ccGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTatat                                                                  <  1:720536/60‑1 (MQ=255)
           ccGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTgg                                                         >  1:5026920/1‑68 (MQ=255)
             gAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCt                                                           >  1:575515/1‑65 (MQ=255)
             gAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaa                                                      >  1:6663331/1‑70 (MQ=255)
              aaGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaa                                                     >  1:3669836/1‑70 (MQ=255)
               aGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaaa                                                    >  1:2762735/1‑70 (MQ=255)
                ggCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACa                                                  <  1:1444824/71‑1 (MQ=255)
                    cTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTc                                                             >  1:7039729/1‑56 (MQ=255)
                     tGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgc                                             >  1:6751202/1‑71 (MQ=255)
                      ggCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgct                                            >  1:1135771/1‑71 (MQ=255)
                      ggCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgc                                             <  1:5559914/70‑1 (MQ=255)
                       gCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgct                                            <  1:1093096/70‑1 (MQ=255)
                       gCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgc                                             <  1:2092980/69‑1 (MQ=255)
                        cTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgctgc                                          <  1:689083/71‑1 (MQ=255)
                         tAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTg                                                          >  1:247955/1‑54 (MQ=255)
                         tAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTg                                                          >  1:7686433/1‑54 (MQ=255)
                         tAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTg                                                          >  1:3809555/1‑54 (MQ=255)
                         tAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgctgc                                          >  1:4215915/1‑70 (MQ=255)
                         tAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgctgc                                          >  1:6501423/1‑70 (MQ=255)
                          aaCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTc                                                             <  1:2417371/50‑1 (MQ=255)
                          aaCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGcc                                         >  1:5391714/1‑70 (MQ=255)
                          aaCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCa                                        >  1:1043875/1‑71 (MQ=255)
                           aCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACa                                                  >  1:153834/1‑60 (MQ=255)
                           aCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCa                                        <  1:2350488/70‑1 (MQ=255)
                            cATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCAtt                                      <  1:1677456/71‑1 (MQ=255)
                             aTGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAAc                                                   <  1:5609302/57‑1 (MQ=255)
                             aTGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTg                                     >  1:4784507/1‑71 (MQ=255)
                              tGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAATAAc                                                   <  1:918876/56‑1 (MQ=255)
                                aGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCt                                                           >  1:3575371/1‑46 (MQ=255)
                                aGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTc                                   >  1:2569237/1‑70 (MQ=255)
                                 ggAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTc                                   <  1:6451469/69‑1 (MQ=255)
                                 ggAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCg                                  >  1:3235550/1‑70 (MQ=255)
                                 ggAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCg                                  >  1:1389588/1‑70 (MQ=255)
                                   aaaTCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTgg                                                         >  1:746587/1‑44 (MQ=255)
                                   aaaTCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAAc                               <  1:2979866/71‑1 (MQ=255)
                                    aaTCTGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAAc                               >  1:3427437/1‑70 (MQ=255)
                                    aaTCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAAcc                              <  1:1401370/71‑1 (MQ=255)
                                    aaTCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAAc                               >  1:7826799/1‑70 (MQ=255)
                                     aTCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtg                                              >  1:6479040/1‑54 (MQ=255)
                                     aTCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCa                             <  1:3848125/71‑1 (MQ=255)
                                     aTCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCa                             >  1:610632/1‑71 (MQ=255)
                                      tCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaa                                                       <  1:1744006/44‑1 (MQ=255)
                                         gggTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGaa                                >  1:3551687/1‑64 (MQ=255)
                                         gggTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATccc                         >  1:6100613/1‑71 (MQ=255)
                                          ggTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaaa                                                    >  1:5834951/1‑43 (MQ=255)
                                          ggTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCt                        <  1:2996131/71‑1 (MQ=255)
                                           gTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTa                       <  1:1786185/71‑1 (MQ=255)
                                           gTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTa                       <  1:7488971/71‑1 (MQ=255)
                                            ttATCGGTAAGGGTGGTTTCGCTATATCGTTCCTg                                                          >  1:314490/1‑35 (MQ=38)
                                            ttATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtcctgc                                          <  1:2181976/51‑1 (MQ=255)
                                            ttATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTaa                      >  1:669401/1‑71 (MQ=255)
                                             tATCGGTAAGGGTGGTTTCGCTATATCGTTCCTgg                                                         <  1:562577/35‑2 (MQ=37)
                                             tATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCg                                  <  1:1000463/58‑1 (MQ=255)
                                              aTCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTc                                   >  1:3094463/1‑56 (MQ=255)
                                              aTCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATcc                          >  1:252989/1‑65 (MQ=255)
                                                 ggTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGtt                 <  1:6907311/70‑1 (MQ=255)
                                                 ggTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGtt                 <  1:4113271/70‑1 (MQ=255)
                                                  gTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACa                                                  <  1:1904254/37‑1 (MQ=38)
                                                  gTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCAtt                                      >  1:2278070/1‑49 (MQ=38)
                                                  gTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGtt                 <  1:6504901/70‑1 (MQ=255)
                                                  gTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTg                >  1:1068091/1‑71 (MQ=255)
                                                   tAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTgg               >  1:7910880/1‑71 (MQ=255)
                                                    aaGGGTGGTTTCGCTATATCCTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGt              >  1:3122959/1‑71 (MQ=255)
                                                     aGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGtt             <  1:3325178/71‑1 (MQ=255)
                                                     aGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGtt             <  1:6326306/71‑1 (MQ=255)
                                                      gggTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTa            <  1:5308064/71‑1 (MQ=255)
                                                      gggTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTa            <  1:5880470/71‑1 (MQ=255)
                                                      gggTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTa            <  1:2100232/71‑1 (MQ=255)
                                                       ggtggtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAAcc                              <  1:1004081/52‑1 (MQ=39)
                                                       ggtggtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAAcc                              <  1:6253849/52‑1 (MQ=39)
                                                       ggtggtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAAcc                              <  1:4588466/52‑1 (MQ=39)
                                                        gtggtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATc          <  1:1776169/71‑1 (MQ=255)
                                                        gtggtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATc          <  1:5788382/71‑1 (MQ=255)
                                                         tggtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCg                                  >  1:4481392/1‑46 (MQ=38)
                                                         tggtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATc          >  1:747929/1‑70 (MQ=255)
                                                          ggtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCg                                  <  1:6257492/45‑1 (MQ=38)
                                                          ggtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTgg               >  1:7719426/1‑64 (MQ=255)
                                                           gtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGGCATTg                                     <  1:7548067/41‑1 (MQ=25)
                                                           gtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTg                                     <  1:5235715/41‑1 (MQ=38)
                                                           gtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAg       >  1:5076944/1‑71 (MQ=255)
                                                            tttCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGtt                 <  1:4633068/60‑1 (MQ=255)
                                                            tttCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGtt                 <  1:36419/60‑1 (MQ=255)
                                                            tttCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGt      <  1:5487102/71‑1 (MQ=255)
                                                            gttCGCTATATCTTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGt      <  1:3780850/70‑1 (MQ=255)
                                                             ttCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGt                                    >  1:5551088/1‑40 (MQ=37)
                                                             ttCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGt                                    >  1:889087/1‑40 (MQ=37)
                                                             ttCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCAt                            >  1:6997076/1‑48 (MQ=38)
                                                              tCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTa     <  1:4058045/70‑1 (MQ=255)
                                                                gCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAg       >  1:6388596/1‑66 (MQ=255)
                                                                gCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGt      >  1:285434/1‑67 (MQ=255)
                                                                gCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAg   <  1:4144681/70‑1 (MQ=255)
                                                                gCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGa  >  1:2758856/1‑71 (MQ=255)
                                                                   |                                                                   
GGCCATGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCAGTAAGGGTGGTTTCGCTGTATCGTTCCTGAAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGA  >  AP009048/3684638‑3684772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: