| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,684,885 | T→C | 100% | pseudogene (174/582 nt) pseudogene (1237/1645 nt) |
ilvG ← ilvG ← |
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,684,885 | 0 | T | C | 100.0% | 74.5 / NA | 23 | pseudogene (174/582 nt) pseudogene (1237/1645 nt) | ilvG ilvG | ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (11/12); total (11/12) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACAACGGTATCGTTCGGTCGCGCGACTTGTGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTACCTAAACCGCTGGAGGTGATGAAATTTTCCG > AP009048/3684818‑3684952 | gCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGcgcg > 1:6762192/1‑71 (MQ=255) acaTTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTgcgcgc < 1:6498195/70‑1 (MQ=255) caTTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGcc < 1:5061906/70‑1 (MQ=255) tcatGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAccgc > 1:3448159/1‑70 (MQ=255) tcatGAAAGAGCAGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAccgcc > 1:3051078/1‑71 (MQ=255) agagCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGt > 1:7206406/1‑70 (MQ=255) agCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCa < 1:2111104/56‑1 (MQ=255) agCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTaaa > 1:3706843/1‑71 (MQ=255) gagaTACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAccgccg > 1:6883328/1‑52 (MQ=255) aGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAccgccgc < 1:3683505/46‑1 (MQ=255) cgaCGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCt < 1:5949070/66‑1 (MQ=37) cgaCGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTa < 1:1341997/67‑1 (MQ=38) gaCGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTaagc < 1:7550797/70‑3 (MQ=38) aTCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTaag > 1:4074509/1‑63 (MQ=37) tCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTg > 1:2848318/1‑69 (MQ=37) gTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAgg > 1:349915/1‑71 (MQ=38) tttggTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTg < 1:3805741/63‑1 (MQ=37) tCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtgatga < 1:5247762/71‑1 (MQ=38) gcgcgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAg > 1:2443670/1‑62 (MQ=37) gcgcgACTTGCGCGCCAACCGCAGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGaaa < 1:4740770/71‑1 (MQ=38) cgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAgg < 1:7793891/60‑1 (MQ=37) cgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGaaa < 1:7804683/68‑1 (MQ=38) gACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCa < 1:2798134/38‑1 (MQ=255) ttGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtgat > 1:6536563/1‑60 (MQ=37) ttGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCg > 1:69824/1‑71 (MQ=38) | GCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACAACGGTATCGTTCGGTCGCGCGACTTGTGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTACCTAAACCGCTGGAGGTGATGAAATTTTCCG > AP009048/3684818‑3684952 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |