| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,685,100 | A→C | 100% | pseudogene (1022/1645 nt) | ilvG ← | ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,685,100 | 0 | A | C | 100.0% | 401.9 / NA | 114 | pseudogene (1022/1645 nt) | ilvG | ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (60/54); total (60/54) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 114 total aligned reads displayed.
ACGATCCGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGAATGTTCATCACGCAGCTGCGCGCAGTGTTGCTGCCAGTCATTG‑‑ATTTAACGGCTGCTGTAATGC > AP009048/3685032‑3685164 | aCGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCAt > 1:6008180/1‑71 (MQ=255) cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCAt < 1:6492034/70‑1 (MQ=255) cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCAt < 1:5158066/70‑1 (MQ=255) cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCAt < 1:7427178/70‑1 (MQ=255) cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCAt < 1:2312094/70‑1 (MQ=255) aTCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGtt > 1:3519434/1‑71 (MQ=255) aTCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGtt > 1:5768425/1‑71 (MQ=255) tctgACAGTTGTTTTAACAACAACGGCGCGTAGATAG‑ATCACCGGGATGGTCGTAACGCCAGGCATg < 1:2080042/64‑1 (MQ=255) ctgACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTtca > 1:5366598/3‑71 (MQ=255) tgACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTtcat > 1:6743385/2‑71 (MQ=255) gACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTtcat > 1:7773147/1‑70 (MQ=255) aCAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGtt < 1:2138576/66‑1 (MQ=255) aCAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGt < 1:5683150/65‑1 (MQ=255) aCAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTtcatc > 1:5919733/1‑70 (MQ=255) aCAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTtcat < 1:3872106/69‑1 (MQ=255) aCAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTtcat < 1:5254029/69‑1 (MQ=255) aGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCAt > 1:1314931/1‑61 (MQ=255) aGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCAt > 1:6317666/1‑61 (MQ=255) gttgttTTAACAACAACGTCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCA‑‑TTCATCACGCAg < 1:744490/71‑1 (MQ=255) gttgttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGc < 1:7837720/71‑1 (MQ=255) ttgttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTtcatca < 1:4845487/67‑1 (MQ=255) ttgttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTtcatca < 1:2705632/67‑1 (MQ=255) ttgttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACg > 1:58068/1‑69 (MQ=255) ttgttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACg > 1:6902435/1‑69 (MQ=255) ttgttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACg > 1:659455/1‑69 (MQ=255) ttgttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACg > 1:7119292/1‑69 (MQ=255) ttgttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGc > 1:3235170/1‑70 (MQ=255) ttgttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGc > 1:6734185/1‑70 (MQ=255) tgttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAg > 1:3154661/1‑71 (MQ=255) tgttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAg > 1:2777834/1‑71 (MQ=255) gttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAg < 1:2189639/70‑1 (MQ=255) gttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAg < 1:7424216/70‑1 (MQ=255) gttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAg < 1:7456671/70‑1 (MQ=255) gttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAg < 1:7549200/70‑1 (MQ=255) gttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAg > 1:1412301/1‑70 (MQ=255) gttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGc > 1:7235706/1‑71 (MQ=255) gttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGc > 1:7841603/1‑71 (MQ=255) ttttAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGc < 1:3831883/70‑1 (MQ=255) ttttAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCt < 1:1721398/71‑1 (MQ=255) ttttAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCt < 1:5881887/71‑1 (MQ=255) ttttAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCt < 1:196575/71‑1 (MQ=255) ttAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTgc > 1:5065743/1‑71 (MQ=255) tAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAg > 1:6959228/1‑66 (MQ=255) tAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTgcg > 1:1758453/1‑71 (MQ=255) tAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTgcg > 1:7391861/1‑71 (MQ=255) tAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTgc > 1:7871457/1‑70 (MQ=255) aacaacaacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGtt > 1:1170109/1‑55 (MQ=255) aacaacaacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTtcatca > 1:2226395/1‑60 (MQ=255) acaacaacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGt > 1:4850628/1‑53 (MQ=255) acaacaacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGt > 1:3009933/1‑53 (MQ=255) aacaacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATg > 1:6175742/1‑50 (MQ=255) aacaacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTgcgcg > 1:1106640/1‑69 (MQ=255) aacaacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCa < 1:7805431/71‑1 (MQ=255) acaacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTgc > 1:632864/1‑65 (MQ=255) acaacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAg > 1:5426722/1‑71 (MQ=255) caacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAgt > 1:3545833/1‑71 (MQ=255) caacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAgt < 1:5772435/71‑1 (MQ=255) caacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAgt < 1:4802916/71‑1 (MQ=255) aacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAg < 1:7084369/69‑1 (MQ=255) aacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAg < 1:2774711/69‑1 (MQ=255) acGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAgtgt < 1:6925997/71‑1 (MQ=255) acGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAgtgt < 1:4088625/71‑1 (MQ=255) acGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAgtgt < 1:5873064/71‑1 (MQ=255) acGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAgtgt < 1:4264202/71‑1 (MQ=255) acGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAgtgt < 1:6121137/71‑1 (MQ=255) cgcgTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGt > 1:175694/1‑43 (MQ=255) cgcgTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGt > 1:4129617/1‑43 (MQ=255) cgcgTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGt > 1:188530/1‑43 (MQ=255) cgcgTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGt > 1:5193666/1‑43 (MQ=255) gcgTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGc < 1:1058774/51‑1 (MQ=255) cgTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTagctgc < 1:6498304/71‑1 (MQ=255) gTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTagctgc > 1:6190610/1‑70 (MQ=255) gATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCAc > 1:1675224/1‑44 (MQ=255) gATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCAc > 1:7166262/1‑44 (MQ=255) gATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAg < 1:1063121/70‑1 (MQ=255) gATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAg < 1:3453725/70‑1 (MQ=255) aGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCAt > 1:6682692/1‑71 (MQ=255) aGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCAt > 1:4947909/1‑71 (MQ=255) cGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTg < 1:717215/71‑1 (MQ=255) cGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGAAGTGTAGCTGCCATTc < 1:1674265/67‑1 (MQ=38) tCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCt < 1:5495199/43‑1 (MQ=255) tCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTgcgc < 1:5061712/47‑1 (MQ=255) cACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTG‑‑Ata > 1:915971/1‑70 (MQ=255) cACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCAAGTCAt > 1:2554295/1‑66 (MQ=39) aCCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCAt > 1:5350024/1‑65 (MQ=39) aCCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCAt > 1:629169/1‑65 (MQ=39) aCCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTG‑‑Atat > 1:1666038/1‑69 (MQ=255) aCCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTG‑‑Atat > 1:6677202/1‑69 (MQ=255) ccGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAg < 1:2710840/60‑1 (MQ=39) ccGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAg < 1:1213268/60‑1 (MQ=39) ggTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTG‑‑a > 1:2641506/1‑60 (MQ=39) gTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCAtt < 1:2667348/57‑1 (MQ=38) gTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGgctgc < 1:4013012/70‑1 (MQ=255) aaCGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCa > 1:2420548/1‑50 (MQ=38) aaCGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGGctgctg > 1:533600/1‑70 (MQ=255) gCCAGGCATGTTGATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCAtt > 1:7138863/1‑49 (MQ=38) cAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGGCTGCTGTAATGc < 1:3008713/71‑1 (MQ=255) cAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTG‑‑a > 1:2956650/1‑49 (MQ=39) cAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTG‑‑a > 1:5460674/1‑49 (MQ=39) cAGGCATGTTAATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGGCTGCTGTAATGc < 1:230822/71‑1 (MQ=255) | ACGATCCGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGAATGTTCATCACGCAGCTGCGCGCAGTGTTGCTGCCAGTCATTG‑‑ATTTAACGGCTGCTGTAATGC > AP009048/3685032‑3685164 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |