| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,685,312 | G→A | 100% | pseudogene (810/1645 nt) pseudogene (810/984 nt) |
ilvG ← ilvG ← |
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,685,312 | 0 | G | A | 100.0% | 335.3 / NA | 114 | pseudogene (810/1645 nt) pseudogene (810/984 nt) | ilvG ilvG | ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (65/49); total (65/49) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 114 total aligned reads displayed.
ATGGATAACACTGGCGTGTGGCGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGCAGGTCACACTCCTGCACCGCGAAGTTTGCCGCTTTGGTGCCGTGCATCCC > AP009048/3685246‑3685377 | atGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCa > 1:4992136/1‑71 (MQ=255)atGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCa > 1:2959886/1‑71 (MQ=255) tGGATAACACTGGCGTTTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCa < 1:274471/70‑1 (MQ=38) tGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCa > 1:1165875/1‑70 (MQ=255) tGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCa > 1:5888872/1‑70 (MQ=255) tGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCa > 1:2896827/1‑70 (MQ=255) tGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCa < 1:177804/70‑1 (MQ=255) tGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCAc > 1:4373000/1‑71 (MQ=255) ggATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACg > 1:7537154/1‑71 (MQ=255) gATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACgg < 1:1736195/71‑1 (MQ=255) aaCACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGa > 1:5924750/1‑71 (MQ=255) aaCACTGGCGTGTGGTGCGAAGGTCTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGa > 1:6560608/1‑71 (MQ=39) cacTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGAt > 1:3817702/1‑70 (MQ=255) cacTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATc < 1:1979064/71‑1 (MQ=255) gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACgg < 1:7527975/61‑1 (MQ=255) gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagcaa < 1:6025959/71‑2 (MQ=255) gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc < 1:3134831/69‑1 (MQ=255) gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc < 1:4076190/69‑1 (MQ=255) cGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagcaag < 1:788848/71‑3 (MQ=39) gtgtGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGt < 1:3514903/71‑1 (MQ=39) tgtgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTc < 1:7524194/71‑1 (MQ=39) ggtgcgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGt < 1:4751169/64‑1 (MQ=38) ggtgcgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGt < 1:2709160/64‑1 (MQ=38) gtgcgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCgcac < 1:5516327/69‑1 (MQ=38) tgcgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcg > 1:7376269/2‑66 (MQ=38) gcgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTc > 1:6778639/1‑71 (MQ=39) cgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTcc < 1:1730356/71‑1 (MQ=39) aaGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGTCGATCAGCAAGTCGCACTCCTg > 1:7710363/1‑71 (MQ=38) aaGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcag < 1:4590817/56‑1 (MQ=255) aaGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCAAGGCGATcag < 1:2303318/56‑1 (MQ=38) gtgtTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc < 1:1328328/54‑1 (MQ=255) gtTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcg > 1:2809863/1‑71 (MQ=39) ttCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagcaa > 1:59547/1‑52 (MQ=255) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc > 1:1200367/1‑49 (MQ=255) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCgcac > 1:2002134/1‑58 (MQ=37) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCgcac > 1:2291667/1‑58 (MQ=37) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcgcg > 1:1512528/1‑70 (MQ=38) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcgcg > 1:7826508/1‑70 (MQ=38) aGTTTGCCGGTCACCCGGTCATCAAAACGTGCAcc > 1:6005267/1‑35 (MQ=255) aGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATc > 1:6474382/1‑45 (MQ=255) aGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTc > 1:41393/1‑59 (MQ=37) aGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTc > 1:3524231/1‑59 (MQ=37) gTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAg < 1:3341375/71‑1 (MQ=39) tttGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGt < 1:229925/71‑1 (MQ=39) tttGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGt < 1:2325498/71‑1 (MQ=39) ttGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGtt > 1:3980775/1‑71 (MQ=39) ttGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGtt > 1:7814005/1‑71 (MQ=39) tGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttc > 1:3920229/1‑70 (MQ=255) tGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttc > 1:5509766/1‑70 (MQ=255) tGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttc > 1:4550700/1‑70 (MQ=255) tGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGAGATaagca > 1:5760670/1‑45 (MQ=25) ccGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACt < 1:2282948/52‑1 (MQ=37) ccGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttcg < 1:6218120/70‑3 (MQ=255) ccGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGc < 1:3714712/71‑1 (MQ=255) cGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAg < 1:4483011/65‑1 (MQ=39) ggTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCg > 1:2999534/1‑71 (MQ=255) ggTCACCAGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCg > 1:338462/1‑71 (MQ=255) gTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCg > 1:243231/1‑70 (MQ=255) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGtt > 1:4131839/1‑63 (MQ=38) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGtt > 1:5468881/1‑63 (MQ=38) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCtt > 1:1201739/1‑71 (MQ=255) aCCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttcg > 1:3479111/1‑62 (MQ=38) aCCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCtt < 1:839480/70‑1 (MQ=255) aCCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCtt < 1:2599447/70‑1 (MQ=255) ccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc < 1:7124118/66‑1 (MQ=255) ccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCtt < 1:7846984/68‑1 (MQ=255) ccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCtt < 1:4992140/68‑1 (MQ=255) cGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc < 1:5682448/65‑1 (MQ=255) ggTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTg > 1:700712/1‑46 (MQ=37) ggTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTg > 1:102968/1‑71 (MQ=255) ggTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTg < 1:4600237/71‑1 (MQ=255) tcatcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCt < 1:4636691/63‑1 (MQ=255) tcatcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCt < 1:5491138/63‑1 (MQ=255) tcatcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGcc < 1:3003174/71‑1 (MQ=255) catcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCg > 1:1327388/1‑60 (MQ=255) atcaAAACGTGCACCCACGTCGATCAGCAAGTCGCACTCCTGCACCGCGAAg > 1:372607/1‑52 (MQ=255) atcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGcca < 1:1768479/70‑2 (MQ=255) atcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGcca < 1:7860857/70‑2 (MQ=255) tcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttcg < 1:6349189/55‑3 (MQ=255) tcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttcg < 1:2639223/55‑3 (MQ=255) tcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTg < 1:7655727/63‑1 (MQ=255) tcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTg < 1:1143232/63‑1 (MQ=255) tcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATg > 1:2042781/1‑71 (MQ=255) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCAcc > 1:2736121/1‑44 (MQ=37) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttcg < 1:2576814/54‑3 (MQ=38) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGt > 1:7322872/1‑51 (MQ=255) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGt > 1:2915858/1‑51 (MQ=255) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGt > 1:178422/1‑51 (MQ=255) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGt > 1:5757840/1‑51 (MQ=255) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcg > 1:7562995/1‑44 (MQ=37) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcg > 1:5177636/1‑44 (MQ=37) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttc > 1:3857693/1‑51 (MQ=38) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGc > 1:3020324/1‑54 (MQ=37) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCa < 1:5793704/71‑1 (MQ=255) aaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGcc > 1:7438488/1‑54 (MQ=37) aaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGcc > 1:5747646/1‑54 (MQ=37) aaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGcc > 1:5186581/1‑54 (MQ=37) aaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGcc > 1:5502684/1‑54 (MQ=37) aCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGaa > 1:4709585/1‑45 (MQ=38) cGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtcc < 1:7853361/71‑1 (MQ=255) | ATGGATAACACTGGCGTGTGGCGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGCAGGTCACACTCCTGCACCGCGAAGTTTGCCGCTTTGGTGCCGTGCATCCC > AP009048/3685246‑3685377 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |