| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,685,329 | G→A | 100% | pseudogene (793/1645 nt) pseudogene (793/984 nt) |
ilvG ← ilvG ← |
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,685,329 | 0 | G | A | 100.0% | 316.9 / NA | 105 | pseudogene (793/1645 nt) pseudogene (793/984 nt) | ilvG ilvG | ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (63/42); total (63/42) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 114 total aligned reads displayed.
GTGTGGCGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGCAGGTCACACTCCTGCACCGCGAAGTTTGCCGCTTTGGTGCCGTGCATCCCCAGCATGCCCAGATAGTAC > AP009048/3685261‑3685396 | gtgtGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGt < 1:3514903/71‑1 (MQ=39)gtgtGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGt < 1:1625329/71‑1 (MQ=39) tgtgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTc < 1:7524194/71‑1 (MQ=39) ggtgcgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGt < 1:2709160/64‑1 (MQ=38) gtgcgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCgcac < 1:5516327/69‑1 (MQ=38) tgcgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcg > 1:7376269/2‑66 (MQ=38) gcgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTc > 1:6778639/1‑71 (MQ=39) cgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTcc < 1:1730356/71‑1 (MQ=39) aaGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGTCGATCAGCAAGTCGCACTCCTg > 1:7710363/1‑71 (MQ=38) gtTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcg > 1:2809863/1‑71 (MQ=39) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCgcac > 1:2002134/1‑58 (MQ=37) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcgcg > 1:1512528/1‑70 (MQ=38) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcgcg > 1:7826508/1‑70 (MQ=38) aGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTc > 1:3524231/1‑59 (MQ=37) aGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTc > 1:41393/1‑59 (MQ=37) aGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcgcg > 1:2445242/1‑69 (MQ=38) gTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAg < 1:3341375/71‑1 (MQ=39) tttGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGt < 1:2325498/71‑1 (MQ=39) tttGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGt < 1:229925/71‑1 (MQ=39) ttGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGtt > 1:3980775/1‑71 (MQ=39) ttGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGtt > 1:7814005/1‑71 (MQ=39) tGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttc > 1:3920229/1‑70 (MQ=255) tGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttc > 1:4550700/1‑70 (MQ=255) tGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttc > 1:5509766/1‑70 (MQ=255) ccGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACt < 1:2282948/52‑1 (MQ=37) ccGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttcg < 1:6218120/70‑3 (MQ=255) ccGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGc < 1:3714712/71‑1 (MQ=255) cGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAg < 1:4483011/65‑1 (MQ=39) ggTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCg > 1:2999534/1‑71 (MQ=255) ggTCACCAGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCg > 1:338462/1‑71 (MQ=255) gTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCg > 1:243231/1‑70 (MQ=255) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGtt > 1:5468881/1‑63 (MQ=38) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCtt > 1:1201739/1‑71 (MQ=255) aCCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttcg > 1:3479111/1‑62 (MQ=38) aCCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCtt < 1:839480/70‑1 (MQ=255) ccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc < 1:3160988/66‑1 (MQ=255) ccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc < 1:7124118/66‑1 (MQ=255) ccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCtt < 1:4992140/68‑1 (MQ=255) ccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCtt < 1:7846984/68‑1 (MQ=255) ccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTgg > 1:3733023/1‑71 (MQ=255) cGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc < 1:5682448/65‑1 (MQ=255) ggTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTg > 1:700712/1‑46 (MQ=37) ggTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTg > 1:102968/1‑71 (MQ=255) ggTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTg < 1:4600237/71‑1 (MQ=255) tcatcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCt < 1:4636691/63‑1 (MQ=255) tcatcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGcc < 1:3003174/71‑1 (MQ=255) catcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCg > 1:1327388/1‑60 (MQ=255) atcaAAACGTGCACCCACGTCGATCAGCAAGTCGCACTCCTGCACCGCGAAg > 1:372607/1‑52 (MQ=255) atcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGcca < 1:1768479/70‑2 (MQ=255) atcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGcca < 1:7860857/70‑2 (MQ=255) tcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttcg < 1:2639223/55‑3 (MQ=255) tcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttcg < 1:6349189/55‑3 (MQ=255) tcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTg < 1:7655727/63‑1 (MQ=255) tcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTg < 1:1143232/63‑1 (MQ=255) tcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATg > 1:2042781/1‑71 (MQ=255) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCAcc > 1:2736121/1‑44 (MQ=37) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttcg < 1:2576814/54‑3 (MQ=38) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGt > 1:5757840/1‑51 (MQ=255) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGt > 1:178422/1‑51 (MQ=255) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcg > 1:5177636/1‑44 (MQ=37) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcg > 1:7562995/1‑44 (MQ=37) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttc > 1:3857693/1‑51 (MQ=38) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGc > 1:3020324/1‑54 (MQ=37) aaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGcc > 1:7438488/1‑54 (MQ=37) aaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGcc > 1:5747646/1‑54 (MQ=37) aaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGcc > 1:5502684/1‑54 (MQ=37) aaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGcc > 1:5186581/1‑54 (MQ=37) aaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAt > 1:6370065/1‑71 (MQ=255) aCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGaa > 1:4709585/1‑45 (MQ=38) cGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtcc < 1:7853361/71‑1 (MQ=255) tGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGaa > 1:4934541/1‑42 (MQ=37) gcacccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAg > 1:5829519/4‑71 (MQ=255) cacccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAg < 1:2233132/68‑1 (MQ=255) cacccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGc < 1:911200/69‑1 (MQ=255) acccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCa > 1:6571501/2‑71 (MQ=255) cccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCAt > 1:5251957/1‑71 (MQ=255) ccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAg < 1:3884402/67‑1 (MQ=255) ccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATg > 1:3279873/1‑71 (MQ=255) cACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGt < 1:2200956/38‑1 (MQ=37) cACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCg > 1:2749049/1‑44 (MQ=37) aCGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCttt < 1:5995968/47‑1 (MQ=37) aCGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCttt < 1:7128221/47‑1 (MQ=37) aCGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGcc > 1:1595726/1‑71 (MQ=255) aCGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGcc > 1:2876687/1‑71 (MQ=255) aCGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGcc > 1:4503070/1‑71 (MQ=255) aCGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGcc > 1:5972980/1‑71 (MQ=255) ggCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGcca > 1:6127474/1‑51 (MQ=38) ggCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtcc > 1:2533134/1‑60 (MQ=255) ggCGATCAGCAAGTCGCACTCATGCACCGCGAAGTTCGCCGCTTTGGTGcca > 1:374327/1‑51 (MQ=255) gCGATCAGCAAGTCGCACTCCTGAACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGcc > 1:6800867/1‑68 (MQ=255) gATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATg < 1:7738714/64‑1 (MQ=255) aTCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGa > 1:4133116/1‑69 (MQ=37) aTCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGa > 1:7096876/1‑69 (MQ=37) tCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCAAGATAg < 1:499/71‑1 (MQ=255) cagcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCttt > 1:3769822/1‑39 (MQ=25) cagcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCttt > 1:316051/1‑39 (MQ=25) cagcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCttt > 1:578820/1‑39 (MQ=25) cagcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAg > 1:3907977/1‑70 (MQ=37) agcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTa < 1:2717652/71‑1 (MQ=37) gcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTAc > 1:2299006/1‑71 (MQ=37) | GTGTGGCGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGCAGGTCACACTCCTGCACCGCGAAGTTTGCCGCTTTGGTGCCGTGCATCCCCAGCATGCCCAGATAGTAC > AP009048/3685261‑3685396 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |