| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,685,369 | G→A | 100% | pseudogene (753/1645 nt) pseudogene (753/984 nt) |
ilvG ← ilvG ← |
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,685,369 | 0 | G | A | 100.0% | 384.9 / NA | 128 | pseudogene (753/1645 nt) pseudogene (753/984 nt) | ilvG ilvG | ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (74/54); total (74/54) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 128 total aligned reads displayed.
TCAAAACGTGCGCCCACGGCGATCAGCAGGTCACACTCCTGCACCGCGAAGTTTGCCGCTTTGGTGCCGTGCATCCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGT > AP009048/3685301‑3685434 | aaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAt > 1:6370065/1‑71 (MQ=255) aCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAttc > 1:7813137/1‑69 (MQ=255) cGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtcc < 1:7853361/71‑1 (MQ=255) gcacccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAg > 1:5829519/4‑71 (MQ=255) cacccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAg < 1:2233132/68‑1 (MQ=255) cccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCAt > 1:5251957/1‑71 (MQ=255) ccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATg > 1:3279873/1‑71 (MQ=255) aCGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGcc > 1:4503070/1‑71 (MQ=255) aCGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGcc > 1:5972980/1‑71 (MQ=255) aCGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGcc > 1:176382/1‑71 (MQ=255) aCGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGcc > 1:1595726/1‑71 (MQ=255) ggCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtcc > 1:2533134/1‑60 (MQ=255) gCGATCAGCAAGTCGCACTCCTGAACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGcc > 1:6800867/1‑68 (MQ=255) gATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATg < 1:7738714/64‑1 (MQ=255) aTCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGa > 1:4133116/1‑69 (MQ=37) cagcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAg > 1:3907977/1‑70 (MQ=37) agcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTa < 1:2717652/71‑1 (MQ=37) gcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTAc > 1:6529870/1‑71 (MQ=37) aagTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACgg < 1:533109/69‑1 (MQ=38) agTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCa > 1:3990766/2‑51 (MQ=37) gTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGAt < 1:6506796/71‑1 (MQ=38) tcgcacTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATa < 1:3663992/68‑1 (MQ=38) cgcacTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATaa > 1:4193225/3‑71 (MQ=38) cgcacTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATaa > 1:492325/3‑71 (MQ=38) gcacTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATaa > 1:7160341/2‑70 (MQ=38) gcacTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATaa < 1:2559868/69‑1 (MQ=38) cacTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGccc < 1:5708752/54‑1 (MQ=37) cacTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATc < 1:5118074/71‑1 (MQ=39) acTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtcc < 1:5580204/43‑1 (MQ=37) acTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtcc < 1:7354928/43‑1 (MQ=37) acTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtcc < 1:3450105/43‑1 (MQ=37) acTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAg < 1:799208/59‑1 (MQ=37) acTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGa > 1:1692568/1‑65 (MQ=38) acTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCt < 1:2066767/71‑1 (MQ=39) acTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCt < 1:2368731/71‑1 (MQ=39) acTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTACCAGCATGCCCAGATAGTACGGATAATCt > 1:341677/1‑71 (MQ=255) tCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATaa < 1:1776554/66‑1 (MQ=38) tCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATaa < 1:7480216/66‑1 (MQ=38) ccTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCt > 1:5155786/1‑71 (MQ=39) cTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCa > 1:666780/1‑44 (MQ=37) cTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCAt > 1:3229005/1‑45 (MQ=37) cTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCtt > 1:5581633/1‑71 (MQ=39) cTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCtt > 1:1060952/1‑71 (MQ=39) cTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCtt > 1:170341/1‑71 (MQ=39) cTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCt > 1:1137174/1‑70 (MQ=38) gCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtccc > 1:2325786/1‑38 (MQ=25) gCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtccc > 1:2335058/1‑38 (MQ=25) gCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtccc > 1:281946/1‑38 (MQ=25) gCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAg < 1:7122152/53‑1 (MQ=37) gCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTc > 1:7309634/1‑70 (MQ=38) cACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTc < 1:2079236/69‑1 (MQ=38) cACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCt < 1:3403484/70‑1 (MQ=38) cACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTa > 1:823899/1‑71 (MQ=39) cACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTa < 1:5512436/71‑1 (MQ=39) aCCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTa > 1:5028690/1‑53 (MQ=37) aCCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTAc < 1:5972151/71‑1 (MQ=39) cgcgAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTg < 1:867716/71‑1 (MQ=39) cgcgAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTg > 1:4042555/1‑71 (MQ=39) gcgAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTgc < 1:4775337/71‑1 (MQ=39) cgAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGc > 1:3272595/1‑39 (MQ=25) cgAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATGGTACGGATAATCTGCTTCTACTgcg > 1:6538432/1‑71 (MQ=38) gAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTgcgc > 1:1194564/1‑71 (MQ=39) aaGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACg < 1:4151084/49‑1 (MQ=25) aGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGccc < 1:4256616/71‑1 (MQ=39) tcgCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAg > 1:4391008/3‑70 (MQ=39) cgCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGc > 1:5352743/2‑54 (MQ=37) cgCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGc > 1:7217210/2‑54 (MQ=37) cgCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGc > 1:2992089/2‑54 (MQ=37) cgCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGcc > 1:3235555/2‑71 (MQ=255) cgCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGcc > 1:7166830/2‑71 (MQ=255) cgCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGcc > 1:6374471/2‑71 (MQ=255) gCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGccc < 1:4626119/71‑1 (MQ=255) gCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGcc < 1:3716843/70‑1 (MQ=255) ccGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTgc > 1:6639028/1‑61 (MQ=255) ccGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTgc > 1:1808230/1‑61 (MQ=255) cGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACgg < 1:3879759/42‑1 (MQ=25) cGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCtt > 1:1408981/1‑71 (MQ=255) gCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTa > 1:2850693/1‑55 (MQ=38) cTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAAt > 1:3143470/1‑45 (MQ=25) cTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAAt > 1:2562787/1‑45 (MQ=25) ttGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAg > 1:6062091/1‑71 (MQ=255) tGGTGCCATGCATTCCCAGCATTCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCa < 1:1577554/69‑1 (MQ=255) tGGTGCCATGCATTCCCAGCATGCCCAGATAGTACgg < 1:7259620/37‑1 (MQ=21) tGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTa > 1:239086/1‑51 (MQ=37) tGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTa > 1:3885866/1‑51 (MQ=37) tGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTa > 1:4034012/1‑51 (MQ=37) tGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCa > 1:7274743/1‑60 (MQ=38) tGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCa < 1:7381309/69‑1 (MQ=255) tGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCa < 1:6780779/69‑1 (MQ=255) tGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCa < 1:6473078/69‑1 (MQ=255) tGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCa < 1:3937387/69‑1 (MQ=255) tGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGc > 1:3763128/1‑71 (MQ=255) ggTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGc < 1:6183539/70‑1 (MQ=255) gTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCg < 1:2986837/70‑1 (MQ=255) gTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCg < 1:4896586/70‑1 (MQ=255) gTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCg < 1:2111938/70‑1 (MQ=255) gTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCg < 1:7872866/70‑1 (MQ=255) gTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCg < 1:4905864/70‑1 (MQ=255) gTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGt < 1:440795/71‑1 (MQ=255) tGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGt < 1:3174465/70‑1 (MQ=255) | TCAAAACGTGCGCCCACGGCGATCAGCAGGTCACACTCCTGCACCGCGAAGTTTGCCGCTTTGGTGCCGTGCATCCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGT > AP009048/3685301‑3685434 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |