| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,808,227 | T→A | 6.1% | intergenic (+31/+22) | yicI → / ← yicH | predicted alpha‑glucosidase/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,808,227 | 0 | T | A | 6.1% | 182.5 / 3.8 | 66 | intergenic (+31/+22) | yicI/yicH | predicted alpha‑glucosidase/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (44/18); new base A (2/2); total (46/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
AAGCCTCAAGGGAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCACCGCTGGAAACGATGC > AP009048/3808161‑3808295 | aaGCCTCAAGGGAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTc > 1:2787541/1‑68 (MQ=255) aGCCTCAAGGGAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTAc > 1:6236883/1‑71 (MQ=255) gCCTCAAGGGAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTAcc > 1:6549137/1‑71 (MQ=255) gCCTCAAGGGAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTAcc > 1:7555915/1‑71 (MQ=255) gCCTCAAGGGAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGACTCAGACGTAcc < 1:423211/71‑1 (MQ=38) gCCTCAAGGGAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGACTCAGACGTAcc < 1:5803287/71‑1 (MQ=38) cAAGGGAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGc > 1:4997249/1‑71 (MQ=255) cAAGGGAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGc > 1:6195289/1‑71 (MQ=255) cAAGGGAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGACTCAGACGTACCCg > 1:7756496/1‑69 (MQ=38) aaGGGAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGc > 1:6906619/1‑70 (MQ=255) aaGGGAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGc > 1:6690556/1‑70 (MQ=255) aaGGGAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGc > 1:2282972/1‑70 (MQ=255) aaGGGAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGc > 1:5688892/1‑70 (MQ=255) aGGGAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCga < 1:4471548/71‑1 (MQ=255) aGGGAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCga < 1:1075503/71‑1 (MQ=255) aGGGAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCga < 1:5915357/71‑1 (MQ=255) ggAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCgaga < 1:5081175/71‑1 (MQ=255) gAATGCGCTGACAATTACTTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCgaga < 1:870743/70‑1 (MQ=255) cgcTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATg > 1:4655483/1‑71 (MQ=255) tcTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTa < 1:3971572/53‑1 (MQ=255) cTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGtt < 1:4384156/71‑1 (MQ=255) cTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGtt > 1:2735854/1‑71 (MQ=255) gACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTa > 1:1648004/1‑71 (MQ=255) cAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTAcc < 1:5322553/71‑1 (MQ=255) aaTTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTc > 1:5211141/1‑45 (MQ=255) aaTTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTc > 1:1384909/1‑45 (MQ=255) aaTTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTc > 1:2180168/1‑45 (MQ=255) aaTTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGt < 1:1969392/47‑1 (MQ=255) aaTTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGt < 1:5859782/47‑1 (MQ=255) aCGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCAcc > 1:3899586/1‑71 (MQ=255) cGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCgaga > 1:4620975/1‑53 (MQ=255) cGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCg > 1:6084815/1‑50 (MQ=255) ttGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATg < 1:7910088/57‑1 (MQ=255) gTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTc > 1:1526301/1‑71 (MQ=255) gTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTc > 1:3326855/1‑71 (MQ=255) aTCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGt > 1:3785333/1‑53 (MQ=255) aTCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCg < 1:6553923/70‑1 (MQ=255) aTCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGt > 1:163905/1‑71 (MQ=255) tATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGa > 1:5758721/1‑71 (MQ=255) aTTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCAc > 1:1109696/1‑58 (MQ=255) aTTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCAc > 1:1902647/1‑58 (MQ=255) ttGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGAt < 1:2234211/70‑1 (MQ=255) tGGAATGCCGGGTACGTCTGTGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAAc > 1:5443724/1‑71 (MQ=255) tGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAAc < 1:127729/71‑1 (MQ=255) tGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAAc > 1:2969868/1‑71 (MQ=255) aTGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCa > 1:4914250/1‑71 (MQ=255) tGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTAc < 1:3042175/43‑1 (MQ=255) tGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTcc > 1:6923110/1‑55 (MQ=255) tGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCAc > 1:4945960/1‑71 (MQ=255) tGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCAc > 1:2299821/1‑71 (MQ=255) ccGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCACCg > 1:6714431/1‑71 (MQ=255) gggTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCa > 1:1241755/1‑43 (MQ=255) gTACGACTCAGACGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCACCGCt > 1:4718005/1‑69 (MQ=38) aCGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCACCg < 1:2853359/65‑1 (MQ=255) aCGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCACCGCTGGa > 1:2541920/1‑70 (MQ=255) aCGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCACCGCTGGa > 1:2294425/1‑70 (MQ=255) cGTCTGATTCGTACCCGGCGAGATTGATGTTTACCGc < 1:4281589/37‑1 (MQ=255) gTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCACCGCTGGAAAc > 1:2337390/1‑71 (MQ=255) tCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCa > 1:533082/1‑36 (MQ=255) tCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCACCGCTGGAAAc < 1:3281051/70‑1 (MQ=255) cTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCACCGCTGGAAACGa > 1:477164/1‑71 (MQ=255) tGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCACCGCTGGAAACGAt > 1:4295483/1‑71 (MQ=255) gAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCACCGCt > 1:1956894/1‑61 (MQ=255) gAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCACCGCTGGAAACGATg > 1:6420432/1‑71 (MQ=255) gAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCACCGCTGGAAACGATg > 1:5053049/1‑71 (MQ=255) aGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCACCGCTGGAAACGATGc > 1:4590554/1‑71 (MQ=255) tCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCACCGCTGGAAACg < 1:6606756/65‑1 (MQ=255) tCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCACCGCTGGAAACGATGc > 1:1834425/1‑69 (MQ=255) | AAGCCTCAAGGGAATGCGCTGACAATTACGTTGTAATCTATTGATGGAATGCCGGGTACGTCTGAGTCGTACCCGGCGAGATTGATGTTTACCGCACCGGCTCCGTCGATGTAACTTCACCGCTGGAAACGATGC > AP009048/3808161‑3808295 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |