| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,824,781 | T→C | 100% | D45D (GAT→GAC) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,824,781 | 0 | T | C | 100.0% | 220.3 / NA | 63 | D45D (GAT→GAC) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (36/27); total (36/27) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTTACGCTGAAATCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGATCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTGT > AP009048/3824713‑3824848 | tttACGCTGAAATCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCt < 1:503410/71‑1 (MQ=255)tttACGCTGAAATCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCt < 1:3755355/71‑1 (MQ=255) tACGCTGAAATCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTgg > 1:46043/1‑71 (MQ=255) cGCTGAAATCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGc < 1:6810697/70‑1 (MQ=255) gAAATCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGt > 1:3533381/1‑71 (MQ=255) aaaTCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGtt < 1:2079937/71‑1 (MQ=255) aaaTCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGt < 1:5177973/70‑1 (MQ=255) aaTCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGt < 1:2964647/69‑1 (MQ=255) gggCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCg > 1:3902783/1‑71 (MQ=255) ggCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGt < 1:7361656/71‑1 (MQ=255) ggCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGt > 1:4816497/1‑71 (MQ=255) gcAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGttt > 1:7553326/1‑70 (MQ=255) aaGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTAc > 1:7537933/1‑70 (MQ=255) gCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGTGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCt > 1:5157205/1‑71 (MQ=255) ccccTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCt > 1:6280940/1‑70 (MQ=255) ccTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACt > 1:3755582/1‑49 (MQ=255) ccTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTg > 1:4975178/1‑69 (MQ=255) cTATTTCTTCAACGCCGTGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCt < 1:6023501/67‑1 (MQ=255) cTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAgg > 1:3288320/1‑54 (MQ=255) aTTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGtt > 1:7937652/1‑49 (MQ=255) aTTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGca > 1:3727381/1‑70 (MQ=255) tttCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCa > 1:7167675/1‑43 (MQ=255) tttCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTa > 1:2928471/1‑60 (MQ=255) tttCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGa > 1:5402748/1‑66 (MQ=255) ttcttcAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGcat < 1:5132811/70‑3 (MQ=255) tcttcAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTa > 1:3640850/1‑58 (MQ=255) tcttcAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTa > 1:3918444/1‑58 (MQ=255) cttcAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtg > 1:2781406/1‑70 (MQ=255) cAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCa > 1:7867259/1‑37 (MQ=255) cAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTa > 1:793071/1‑54 (MQ=255) cAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTa > 1:6823887/1‑54 (MQ=255) cAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTa > 1:2729309/1‑54 (MQ=255) aCGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGAt > 1:6427082/1‑71 (MQ=255) gCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGtt < 1:6763654/46‑1 (MQ=255) gCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTc < 1:4457161/71‑1 (MQ=255) cGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTctg < 1:162508/71‑3 (MQ=255) gggCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTgg < 1:626180/71‑1 (MQ=255) gTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAg < 1:1685677/50‑1 (MQ=255) tttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTa > 1:5885076/1‑41 (MQ=255) tttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTc < 1:5515270/62‑1 (MQ=255) tttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTc < 1:1974772/62‑1 (MQ=255) ttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTAc > 1:7338217/1‑41 (MQ=255) ttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGc > 1:2345621/1‑43 (MQ=255) ttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGca > 1:6350946/1‑49 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTAc > 1:3495192/1‑40 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt > 1:5591395/1‑71 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt > 1:4991601/1‑71 (MQ=255) aaTACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCt < 1:2297669/42‑1 (MQ=255) aaTACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCt < 1:7314010/42‑1 (MQ=255) aaTACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtggtg < 1:5477741/54‑1 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGAtt > 1:2715731/1‑54 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGAtt > 1:6469601/1‑54 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTAAGCTGAAGCATTGGTGGAtt > 1:6074408/1‑54 (MQ=255) cGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt < 1:4365068/61‑1 (MQ=255) cGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTATGGCATTGAGTTCGATc > 1:7378645/1‑71 (MQ=255) gggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATct < 1:2245058/71‑1 (MQ=255) ggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctg > 1:3720611/1‑71 (MQ=255) cgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtggtg < 1:7351098/43‑1 (MQ=38) cgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtggtg < 1:7849241/43‑1 (MQ=38) cgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtggtg < 1:2314843/43‑1 (MQ=38) cgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtggtg < 1:1243709/43‑1 (MQ=38) cgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGtt < 1:7706598/60‑1 (MQ=255) cgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGt < 1:4472556/71‑1 (MQ=255) | TTTACGCTGAAATCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGATCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTGT > AP009048/3824713‑3824848 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |