| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,824,814 | G→A | 100% | A56A (GCG→GCA) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,824,814 | 0 | G | A | 100.0% | 365.8 / NA | 120 | A56A (GCG→GCA) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (63/57); total (63/57) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 120 total aligned reads displayed.
CTTCAACGCCGGGCTGTTTAATACCGGGCGCGATCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCC > AP009048/3824748‑3824880 | cttcAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtg > 1:2781406/1‑70 (MQ=255) aCGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGAt > 1:6427082/1‑71 (MQ=255) gCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTc < 1:4457161/71‑1 (MQ=255) cGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTctg < 1:162508/71‑3 (MQ=255) gggCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTgg < 1:626180/71‑1 (MQ=255) tttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTc < 1:5515270/62‑1 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt > 1:5591395/1‑71 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGAtt > 1:2715731/1‑54 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGAtt > 1:6469601/1‑54 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTAAGCTGAAGCATTGGTGGAtt > 1:6074408/1‑54 (MQ=255) gggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATct < 1:2245058/71‑1 (MQ=255) ggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctg > 1:3720611/1‑71 (MQ=255) cgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtggtg < 1:7849241/43‑1 (MQ=38) cgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtggtg < 1:2314843/43‑1 (MQ=38) cgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtggtg < 1:7351098/43‑1 (MQ=38) cgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtggtg < 1:1243709/43‑1 (MQ=38) cgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGtt < 1:7706598/60‑1 (MQ=255) cgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGt < 1:4472556/71‑1 (MQ=255) gaccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctg > 1:5810051/4‑66 (MQ=255) gaccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctg > 1:4908601/4‑66 (MQ=255) gaccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGtt < 1:5647384/68‑1 (MQ=255) gaccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGtt < 1:109486/68‑1 (MQ=255) accTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGttt < 1:674106/69‑1 (MQ=255) accTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGttt < 1:3587428/69‑1 (MQ=255) accTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGttt < 1:1070893/69‑1 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGAtt > 1:5424002/2‑44 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGAtt > 1:3658710/2‑44 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGAtt > 1:4733015/2‑44 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTg > 1:4243558/2‑71 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTg > 1:6319941/2‑71 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTg > 1:3440530/2‑71 (MQ=255) cTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTgg > 1:4504569/1‑71 (MQ=255) ggCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGcc > 1:1417835/1‑71 (MQ=255) ggCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGcc > 1:5042803/1‑71 (MQ=255) ggCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGcc > 1:1018608/1‑71 (MQ=255) gCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt < 1:1276782/52‑1 (MQ=255) aCTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTc > 1:4869227/1‑52 (MQ=255) aCTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTc > 1:3540733/1‑52 (MQ=255) cTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGa < 1:1236114/35‑1 (MQ=255) cTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCg < 1:4544891/52‑1 (MQ=255) cTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCg < 1:7070435/52‑1 (MQ=255) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTc < 1:421893/37‑1 (MQ=255) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGc < 1:1548653/41‑1 (MQ=38) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGc < 1:3538623/41‑1 (MQ=38) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGa > 1:3628244/1‑46 (MQ=39) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGc > 1:3163274/1‑65 (MQ=255) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:723435/1‑71 (MQ=255) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:5525281/1‑71 (MQ=255) gTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCg < 1:5095704/50‑1 (MQ=255) gTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:385460/1‑70 (MQ=255) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCtt < 1:748674/70‑1 (MQ=255) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt < 1:2246073/69‑1 (MQ=255) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt < 1:4734641/69‑1 (MQ=255) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt < 1:4122915/69‑1 (MQ=255) tAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTg > 1:1781273/1‑66 (MQ=255) ggCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACa < 1:1595609/70‑1 (MQ=255) ggCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACa < 1:4130321/70‑1 (MQ=255) gCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt > 1:1647423/1‑47 (MQ=39) cGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGa > 1:2638855/1‑38 (MQ=37) cGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGa > 1:6038630/1‑38 (MQ=37) cGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGa > 1:6484728/1‑38 (MQ=37) cGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAgg < 1:6218397/71‑1 (MQ=255) cGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAgg > 1:7125170/1‑71 (MQ=255) gTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAgg > 1:2588801/1‑70 (MQ=255) tttttACGCTGAAGCATTGGTGGATTCTGGCATTg < 1:2741356/35‑1 (MQ=37) tttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGa < 1:5060298/71‑1 (MQ=255) ttttACGCTTAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGtt < 1:1051937/50‑1 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGttt > 1:4668442/1‑51 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGttt > 1:409129/1‑51 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGttt > 1:7598781/1‑51 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGtt < 1:3356916/50‑1 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGtt < 1:6696869/50‑1 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGtt < 1:3458364/50‑1 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATATGCTGTTTg > 1:5194098/1‑52 (MQ=255) tttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctgct > 1:561537/1‑46 (MQ=39) tttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctgct > 1:5618834/1‑46 (MQ=39) tttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctgct > 1:2290120/1‑46 (MQ=39) tttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctgc > 1:429503/1‑45 (MQ=38) tttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctgc > 1:419416/1‑45 (MQ=38) ttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATc < 1:4271019/70‑1 (MQ=255) tACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTg > 1:6467587/1‑49 (MQ=39) tACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATcct > 1:323743/1‑70 (MQ=255) tACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATcc < 1:5909406/70‑1 (MQ=255) tACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATcc < 1:5676940/70‑1 (MQ=255) tACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATcc < 1:7812763/70‑1 (MQ=255) aCGCTGAAGCATTGGTGGATTCTGGCATTTAGTTAGATCTGCTGTTTGGCCCTGCTTACAAAGGGATccc < 1:6810117/70‑1 (MQ=255) cGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCg < 1:6992070/70‑1 (MQ=255) cTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATc > 1:1425164/1‑36 (MQ=37) cTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATc > 1:6908783/1‑36 (MQ=37) gAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGc > 1:6259606/1‑71 (MQ=255) gAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGc > 1:3264982/1‑71 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCGTGCTTACAAAGGGATCCCGATTGcg < 1:2054228/71‑2 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:4418105/1‑50 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:4115351/1‑50 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:4056052/1‑50 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:2917314/1‑50 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:1044125/1‑50 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcg < 1:694933/71‑2 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcg < 1:6226272/71‑2 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcg < 1:31071/71‑2 (MQ=255) | CTTCAACGCCGGGCTGTTTAATACCGGGCGCGATCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCC > AP009048/3824748‑3824880 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |