| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,824,826 | C→T | 100% | S60S (TCC→TCT) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,824,826 | 0 | C | T | 100.0% | 388.0 / NA | 127 | S60S (TCC→TCT) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (67/60); total (67/60) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 127 total aligned reads displayed.
GGGCTGTTTAATACCGGGCGCGATCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCTG > AP009048/3824758‑3824893 | gggCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTgg < 1:626180/71‑1 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt > 1:5591395/1‑71 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt > 1:4991601/1‑71 (MQ=255) cGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt < 1:4365068/61‑1 (MQ=255) cGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTATGGCATTGAGTTCGATc > 1:7378645/1‑71 (MQ=255) gggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATct < 1:2245058/71‑1 (MQ=255) ggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctg > 1:3720611/1‑71 (MQ=255) cgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGtt < 1:7706598/60‑1 (MQ=255) cgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGt < 1:4472556/71‑1 (MQ=255) gaccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctg > 1:5810051/4‑66 (MQ=255) gaccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctg > 1:4908601/4‑66 (MQ=255) gaccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGtt < 1:5647384/68‑1 (MQ=255) accTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGttt < 1:1070893/69‑1 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTg > 1:3440530/2‑71 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTg > 1:6319941/2‑71 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTg > 1:4243558/2‑71 (MQ=255) ggCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGcc > 1:5042803/1‑71 (MQ=255) ggCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGcc > 1:1018608/1‑71 (MQ=255) ggCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGcc > 1:1417835/1‑71 (MQ=255) ggCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGcc > 1:3956869/1‑71 (MQ=255) gCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt < 1:1276782/52‑1 (MQ=255) aCTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTc > 1:3540733/1‑52 (MQ=255) cTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCg < 1:4544891/52‑1 (MQ=255) cTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCg < 1:7070435/52‑1 (MQ=255) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGc < 1:15436/41‑1 (MQ=38) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGc < 1:3538623/41‑1 (MQ=38) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGc < 1:1548653/41‑1 (MQ=38) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGa > 1:3628244/1‑46 (MQ=39) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGc > 1:4178914/1‑65 (MQ=255) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGc > 1:3163274/1‑65 (MQ=255) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:5525281/1‑71 (MQ=255) gTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCg < 1:7920123/50‑1 (MQ=255) gTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCg < 1:5095704/50‑1 (MQ=255) gTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:385460/1‑70 (MQ=255) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCtt < 1:748674/70‑1 (MQ=255) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt < 1:4122915/69‑1 (MQ=255) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt < 1:4734641/69‑1 (MQ=255) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTa < 1:6276541/71‑1 (MQ=255) tAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTg > 1:1781273/1‑66 (MQ=255) ggCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACaa < 1:4610965/71‑1 (MQ=255) ggCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACa < 1:1595609/70‑1 (MQ=255) ggCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACa < 1:1226505/70‑1 (MQ=255) ggCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACa < 1:5622653/70‑1 (MQ=255) cGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGa > 1:6038630/1‑38 (MQ=37) cGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGa > 1:2638855/1‑38 (MQ=37) cGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAgg > 1:7125170/1‑71 (MQ=255) cGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAgg < 1:6218397/71‑1 (MQ=255) gTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAgg > 1:2588801/1‑70 (MQ=255) tttttACGCTGAAGCATTGGTGGATTCTGGCATTg < 1:2741356/35‑1 (MQ=37) ttttACGCTTAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGtt < 1:1051937/50‑1 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGttt > 1:409129/1‑51 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGttt > 1:4668442/1‑51 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGttt > 1:3282624/1‑51 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGtt < 1:3356916/50‑1 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGtt < 1:6696869/50‑1 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATATGCTGTTTg > 1:5194098/1‑52 (MQ=255) tttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctgct > 1:2290120/1‑46 (MQ=39) tttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctgct > 1:5618834/1‑46 (MQ=39) tttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctgc > 1:419416/1‑45 (MQ=38) tttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctgc > 1:6696574/1‑45 (MQ=38) ttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATc < 1:4271019/70‑1 (MQ=255) tACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTg > 1:6467587/1‑49 (MQ=39) tACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATcc < 1:5909406/70‑1 (MQ=255) tACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATcc < 1:7812763/70‑1 (MQ=255) tACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATcc < 1:5676940/70‑1 (MQ=255) aCGCTGAAGCATTGGTGGATTCTGGCATTTAGTTAGATCTGCTGTTTGGCCCTGCTTACAAAGGGATccc < 1:6810117/70‑1 (MQ=255) cGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCg < 1:6992070/70‑1 (MQ=255) cTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATc > 1:6908783/1‑36 (MQ=37) tGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTg > 1:3574750/1‑71 (MQ=255) gAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGc > 1:3264982/1‑71 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCGTGCTTACAAAGGGATCCCGATTGcg < 1:2054228/71‑2 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:4418105/1‑50 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:1044125/1‑50 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:4115351/1‑50 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:4056052/1‑50 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:2917314/1‑50 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcg < 1:6226272/71‑2 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcg < 1:694933/71‑2 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcg < 1:31071/71‑2 (MQ=255) gcattGGTGGATTCTGGCATTGAGTTAGATATGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcgac > 1:7111299/4‑71 (MQ=255) cattGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACa < 1:27461/49‑1 (MQ=255) attGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAg > 1:6251223/2‑53 (MQ=255) attGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAg > 1:574987/2‑53 (MQ=255) tggtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACaa < 1:3866515/49‑1 (MQ=255) ggtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCtt > 1:2377644/1‑44 (MQ=255) ggtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCtt > 1:7579910/1‑44 (MQ=255) ggtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCgaccact < 1:1561046/70‑2 (MQ=255) gtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAg < 1:3603233/49‑1 (MQ=255) gtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCgaccacta < 1:3596389/70‑3 (MQ=255) gtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCgaccacta < 1:2273078/70‑3 (MQ=255) tggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGc < 1:5486588/40‑1 (MQ=255) tggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTa < 1:7394361/43‑1 (MQ=255) tggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcga > 1:114591/1‑61 (MQ=255) tggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcga > 1:5254947/1‑61 (MQ=255) ggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCg > 1:653700/1‑71 (MQ=255) gATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCtt > 1:3517617/1‑40 (MQ=255) gATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGAt > 1:2378815/1‑56 (MQ=255) ttCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGccg > 1:6600972/1‑69 (MQ=255) ttCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGccg < 1:4991288/71‑3 (MQ=255) ttCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGccg < 1:5586795/71‑3 (MQ=255) | GGGCTGTTTAATACCGGGCGCGATCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCTG > AP009048/3824758‑3824893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |