| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,824,880 | C→G | 100% | A78A (GCC→GCG) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,824,880 | 0 | C | G | 100.0% | 387.0 / NA | 116 | A78A (GCC→GCG) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (60/56); total (60/56) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 123 total aligned reads displayed.
GCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAA > AP009048/3824812‑3824946 | gcattGGTGGATTCTGGCATTGAGTTAGATATGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcgac > 1:7111299/4‑71 (MQ=255) ggtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCgaccact < 1:1561046/70‑2 (MQ=255) gtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCgaccacta < 1:3596389/70‑3 (MQ=255) gtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCgaccacta < 1:2273078/70‑3 (MQ=255) ggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCg > 1:653700/1‑71 (MQ=255) ttCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGccg < 1:5586795/71‑3 (MQ=255) ttCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGccg > 1:6600972/1‑69 (MQ=255) tggCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTAc < 1:247906/62‑1 (MQ=255) tggCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTAc < 1:1684178/62‑1 (MQ=255) tggCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTAc < 1:1887940/62‑1 (MQ=255) gCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCcgtg < 1:7775279/68‑1 (MQ=255) gCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGcg < 1:3230129/71‑2 (MQ=255) gCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGcg > 1:2934536/1‑70 (MQ=255) gCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGc > 1:1456793/1‑70 (MQ=255) cATTGAGTTCGATCTGCTGTTTGGCCATGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGcgc > 1:6920961/1‑69 (MQ=255) aTTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCcgt < 1:4966612/65‑1 (MQ=255) ttGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCgaccacta > 1:2376696/1‑55 (MQ=255) ttGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTg > 1:1648294/1‑71 (MQ=255) gAGTTCGACCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCt > 1:5155731/1‑68 (MQ=255) aGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCg > 1:4732563/1‑71 (MQ=255) aGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCg < 1:7393854/71‑1 (MQ=255) ttCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCg < 1:7503128/69‑1 (MQ=255) tCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTAcc > 1:3413066/1‑53 (MQ=255) tCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTAcc > 1:3422060/1‑53 (MQ=255) tCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTAcc > 1:3031221/1‑53 (MQ=255) tCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTAcc > 1:5566050/1‑53 (MQ=255) cGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGcc > 1:4435907/1‑54 (MQ=255) cGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAg > 1:4655049/1‑70 (MQ=255) cGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAg < 1:3417347/70‑1 (MQ=255) cGATCTGCTGTTTGGACCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAg > 1:1804610/1‑70 (MQ=255) aTCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCcgtg < 1:4663369/56‑1 (MQ=255) ctgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGcc < 1:1423805/48‑2 (MQ=255) ctgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCa < 1:4835809/65‑1 (MQ=255) ctgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCatg < 1:4278503/70‑3 (MQ=255) ctgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCa < 1:3493566/68‑1 (MQ=255) ttGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCcgt > 1:70043/1‑46 (MQ=38) ttGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgacctt > 1:1452605/1‑70 (MQ=255) tGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCcgtg > 1:1006467/1‑46 (MQ=38) tGGCCCTGCTTACAAAGGGATCCCGAATGCGACCACAACCGCCGTGGCGCTgg > 1:226497/1‑53 (MQ=255) ggCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgacctt > 1:3637420/1‑68 (MQ=255) gCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgac < 1:2612917/71‑1 (MQ=255) ccTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct < 1:3231018/71‑1 (MQ=255) cTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctg > 1:3735880/1‑71 (MQ=255) cTGCTTACAAAGGGACCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgacctt < 1:2035985/65‑2 (MQ=255) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCcgt < 1:1921733/39‑1 (MQ=25) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCcgt < 1:943607/39‑1 (MQ=25) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGc > 1:5619638/1‑53 (MQ=37) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct > 1:5172867/1‑69 (MQ=255) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct > 1:4358139/1‑69 (MQ=255) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctga > 1:7863963/1‑70 (MQ=255) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttg < 1:1032786/65‑3 (MQ=255) gCTTACAAAGGGATCCCGATTGCGACCACTACCGcc > 1:4814731/1‑35 (MQ=37) gCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctgac > 1:5771561/1‑69 (MQ=255) gCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgac > 1:6828909/1‑60 (MQ=255) gCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGcc > 1:5187653/1‑71 (MQ=255) cTTACAAAGGGATCCCGATTGCGACCACTACCGCcgt > 1:1691111/1‑37 (MQ=25) cTTACAAAGGGATCCCGATTGCGACCACTACCGCcgt > 1:1065733/1‑37 (MQ=25) cTTACAAAGGGATCCCGATTGCGACCACTACCGCcgt > 1:2677671/1‑37 (MQ=25) cTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGcc < 1:4412129/70‑1 (MQ=255) cTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGcc < 1:1206086/70‑1 (MQ=255) ttACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCa < 1:2236/51‑1 (MQ=37) ttACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCa < 1:526291/51‑1 (MQ=37) ttACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg > 1:3020865/1‑70 (MQ=255) tACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGc < 1:522571/38‑1 (MQ=25) tACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGc < 1:4655563/38‑1 (MQ=25) tACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTa > 1:6568033/1‑71 (MQ=255) aCAAAGGGATCCCGATTGCGACCACTACCGCcgtg > 1:7532440/1‑35 (MQ=25) aCAAAGGGATCCCGATTGCGACCACTACCGCcgtg > 1:2627177/1‑35 (MQ=25) aCAAAGGGATCCCGATTGCGACCACTACCGCCGTGGcg < 1:7390472/38‑2 (MQ=25) aCAAAGGGATCCCGATTGCGACCACTACCGCCGTGGcg < 1:5843187/38‑2 (MQ=25) aCAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCatg < 1:490045/54‑3 (MQ=37) aCAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCatg < 1:236852/54‑3 (MQ=37) aCAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCatg < 1:606600/54‑3 (MQ=37) aCAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTAc > 1:2751745/1‑71 (MQ=255) cAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacc < 1:6556550/62‑1 (MQ=255) cAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTAc < 1:5129861/70‑1 (MQ=255) aaaGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGc < 1:1748530/41‑1 (MQ=21) aaaGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCAt > 1:5148113/1‑48 (MQ=37) aaaGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATc > 1:2007642/1‑49 (MQ=37) aGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAg > 1:1274278/1‑43 (MQ=25) aGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAg > 1:549977/1‑43 (MQ=25) ggATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCttt < 1:2743880/71‑1 (MQ=255) ggATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCttt < 1:6024241/71‑1 (MQ=255) aTCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCa < 1:3094763/41‑1 (MQ=21) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct > 1:314234/1‑53 (MQ=25) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAAcc < 1:4300060/70‑1 (MQ=255) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCg > 1:4198136/1‑71 (MQ=255) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCg > 1:5451746/1‑71 (MQ=255) cGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt > 1:6355198/1‑63 (MQ=255) gATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACt < 1:6144929/59‑1 (MQ=255) gATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCa < 1:309930/71‑1 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTg > 1:6292552/1‑59 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTg > 1:7086049/1‑59 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTg > 1:574762/1‑59 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTg > 1:937073/1‑59 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctg < 1:6134199/50‑1 (MQ=19) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctg < 1:2438601/50‑1 (MQ=19) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt < 1:5684386/60‑1 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt < 1:2963052/60‑1 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa < 1:2633275/71‑1 (MQ=255) | GCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAA > AP009048/3824812‑3824946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |