| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,824,886 | A→T | 100% | T80T (ACA→ACT) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,824,886 | 0 | A | T | 100.0% | 403.8 / ‑6.7 | 137 | T80T (ACA→ACT) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (69/67); total (70/67) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 142 total aligned reads displayed.
GGATTCCGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAAGAAGCAA > AP009048/3824820‑3824953 | ttCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGccg < 1:5586795/71‑3 (MQ=255) ttCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGccg < 1:4991288/71‑3 (MQ=255) tggCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTAc < 1:247906/62‑1 (MQ=255) tggCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTAc < 1:1684178/62‑1 (MQ=255) gCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCcgtg < 1:7775279/68‑1 (MQ=255) gCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGcg > 1:2934536/1‑70 (MQ=255) gCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGcg < 1:3230129/71‑2 (MQ=255) gCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGc > 1:1456793/1‑70 (MQ=255) cATTGAGTTCGATCTGCTGTTTGGCCATGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGcgc > 1:6920961/1‑69 (MQ=255) ttGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTg > 1:1648294/1‑71 (MQ=255) gAGTTCGACCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCt > 1:5155731/1‑68 (MQ=255) aGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCg < 1:7393854/71‑1 (MQ=255) aGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCg > 1:4732563/1‑71 (MQ=255) ttCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCg < 1:7503128/69‑1 (MQ=255) tCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTAcc > 1:3031221/1‑53 (MQ=255) tCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTAcc > 1:3422060/1‑53 (MQ=255) tCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTAcc > 1:5566050/1‑53 (MQ=255) cGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGcc > 1:4435907/1‑54 (MQ=255) cGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAg > 1:4655049/1‑70 (MQ=255) cGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAg < 1:3417347/70‑1 (MQ=255) cGATCTGCTGTTTGGACCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAg > 1:1804610/1‑70 (MQ=255) ctgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGcc < 1:1423805/48‑2 (MQ=255) ctgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCa < 1:4835809/65‑1 (MQ=255) ttGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCcgt > 1:70043/1‑46 (MQ=38) ttGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgacctt > 1:1452605/1‑70 (MQ=255) tGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCcgtg > 1:1006467/1‑46 (MQ=38) ggCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgacctt > 1:3637420/1‑68 (MQ=255) gCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgac < 1:3284182/71‑1 (MQ=255) ccTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct < 1:3231018/71‑1 (MQ=255) cTGCTTACAAAGGGACCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgacctt < 1:2035985/65‑2 (MQ=255) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCcgt < 1:943607/39‑1 (MQ=25) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCcgt < 1:1921733/39‑1 (MQ=25) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct > 1:4358139/1‑69 (MQ=255) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct > 1:5172867/1‑69 (MQ=255) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctga > 1:7863963/1‑70 (MQ=255) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttg < 1:1032786/65‑3 (MQ=255) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttg < 1:4765372/65‑3 (MQ=255) gCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCa > 1:5711953/1‑56 (MQ=255) gCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctgac > 1:5771561/1‑69 (MQ=255) gCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGcc > 1:5187653/1‑71 (MQ=255) gCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGc > 1:6753898/1‑70 (MQ=255) cTTACAAAGGGATCCCGATTGCGACCACTACCGCcgt > 1:1691111/1‑37 (MQ=25) cTTACAAAGGGATCCCGATTGCGACCACTACCGCcgt > 1:1065733/1‑37 (MQ=25) ttACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCa < 1:526291/51‑1 (MQ=37) ttACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCa < 1:2236/51‑1 (MQ=37) ttACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg > 1:3020865/1‑70 (MQ=255) ttACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg > 1:3578159/1‑70 (MQ=255) tACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGc < 1:4655563/38‑1 (MQ=25) tACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGc < 1:522571/38‑1 (MQ=25) tACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTa > 1:6568033/1‑71 (MQ=255) aCAAAGGGATCCCGATTGCGACCACTACCGCcgtg > 1:2627177/1‑35 (MQ=25) aCAAAGGGATCCCGATTGCGACCACTACCGCcgtg > 1:7532440/1‑35 (MQ=25) aCAAAGGGATCCCGATTGCGACCACTACCGCCGTGGcg < 1:5843187/38‑2 (MQ=25) aCAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCatg < 1:606600/54‑3 (MQ=37) aCAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCatg < 1:490045/54‑3 (MQ=37) aCAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCatg < 1:236852/54‑3 (MQ=37) aCAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTAc > 1:2751745/1‑71 (MQ=255) cAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacc < 1:6556550/62‑1 (MQ=255) cAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTAc < 1:5129861/70‑1 (MQ=255) aaaGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGc < 1:1748530/41‑1 (MQ=21) aaaGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATc > 1:2007642/1‑49 (MQ=37) aaaGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTg > 1:4233641/1‑71 (MQ=255) aGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAg > 1:1274278/1‑43 (MQ=25) aGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAg > 1:549977/1‑43 (MQ=25) ggATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCttt < 1:2743880/71‑1 (MQ=255) gATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTa < 1:4548058/71‑1 (MQ=255) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct > 1:3075587/1‑53 (MQ=25) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct > 1:2182999/1‑53 (MQ=25) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct > 1:18395/1‑53 (MQ=25) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAAcc < 1:4300060/70‑1 (MQ=255) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCg > 1:4198136/1‑71 (MQ=255) cGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt > 1:6355198/1‑63 (MQ=255) gATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACt < 1:6144929/59‑1 (MQ=255) gATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGc > 1:6457176/1‑70 (MQ=255) gATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCa < 1:309930/71‑1 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTg > 1:7086049/1‑59 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTg > 1:937073/1‑59 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCa > 1:7111877/1‑70 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctg < 1:2438601/50‑1 (MQ=19) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctg < 1:6134199/50‑1 (MQ=19) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt < 1:2963052/60‑1 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt < 1:5684386/60‑1 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa < 1:2375512/71‑1 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa < 1:2632940/71‑1 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa < 1:2633275/71‑1 (MQ=255) cgaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaagag < 1:6078481/69‑2 (MQ=255) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATgaccttga > 1:6294539/2‑42 (MQ=16) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg < 1:679610/48‑1 (MQ=25) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg < 1:1621867/48‑1 (MQ=25) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg < 1:4040841/48‑1 (MQ=25) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg < 1:4692585/48‑1 (MQ=25) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaagagg > 1:3129186/2‑69 (MQ=255) accacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTaa > 1:6637091/1‑59 (MQ=255) accacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaagagg < 1:4546941/70‑3 (MQ=255) accacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc < 1:1263178/71‑1 (MQ=255) accacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc < 1:4151007/71‑1 (MQ=255) ccacTACCGCCGTGGCGCTGGCGGATCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCa < 1:5470260/71‑1 (MQ=255) ccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGc < 1:5545807/62‑1 (MQ=255) ccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc > 1:5945727/1‑70 (MQ=255) cactACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:4097911/71‑1 (MQ=255) | GGATTCCGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAAGAAGCAA > AP009048/3824820‑3824953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |