| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,824,913 | C→T | 100% | H89H (CAC→CAT) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,824,913 | 0 | C | T | 100.0% | 368.3 / NA | 124 | H89H (CAC→CAT) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (57/67); total (57/67) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 133 total aligned reads displayed.
TTGGCCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAAGAAGCAAAAGACCACGGTGAAGGCGGCAATCT > AP009048/3824849‑3824978 | ttGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgacctt > 1:1452605/1‑70 (MQ=255) ggCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgacctt > 1:3637420/1‑68 (MQ=255) gCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgac < 1:6096595/71‑1 (MQ=255) gCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgac < 1:3284182/71‑1 (MQ=255) cTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctg > 1:3735880/1‑71 (MQ=255) cTGCTTACAAAGGGACCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgacctt < 1:2035985/65‑2 (MQ=255) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct > 1:4358139/1‑69 (MQ=255) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct > 1:5172867/1‑69 (MQ=255) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttg < 1:1032786/65‑3 (MQ=255) gCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgac > 1:6828909/1‑60 (MQ=255) gCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGcc > 1:5187653/1‑71 (MQ=255) gCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGc > 1:6753898/1‑70 (MQ=255) cTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGcc < 1:1323528/70‑1 (MQ=255) cTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGcc < 1:1206086/70‑1 (MQ=255) cTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGcc < 1:4412129/70‑1 (MQ=255) ttACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg > 1:3020865/1‑70 (MQ=255) cAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacc < 1:6556550/62‑1 (MQ=255) aGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt > 1:2793645/1‑71 (MQ=255) ggATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCttt < 1:2743880/71‑1 (MQ=255) ggATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCttt < 1:6024241/71‑1 (MQ=255) gATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTa < 1:4548058/71‑1 (MQ=255) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct > 1:18395/1‑53 (MQ=25) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct > 1:314234/1‑53 (MQ=25) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct > 1:3075587/1‑53 (MQ=25) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAAcc < 1:4300060/70‑1 (MQ=255) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCg > 1:4198136/1‑71 (MQ=255) gATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACt < 1:6144929/59‑1 (MQ=255) gATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGc > 1:6457176/1‑70 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTg > 1:937073/1‑59 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTg > 1:7086049/1‑59 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTg > 1:574762/1‑59 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTg > 1:6292552/1‑59 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCa > 1:7111877/1‑70 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctg < 1:2438601/50‑1 (MQ=19) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt < 1:2963052/60‑1 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt < 1:5684386/60‑1 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa < 1:2375512/71‑1 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa < 1:2632940/71‑1 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa < 1:2633275/71‑1 (MQ=255) gcgaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa > 1:3020275/4‑69 (MQ=255) cgaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaagag < 1:6078481/69‑2 (MQ=255) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATgaccttga > 1:7031577/2‑42 (MQ=16) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATgaccttga > 1:166434/2‑42 (MQ=16) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATgaccttga > 1:1739204/2‑42 (MQ=16) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATgaccttga > 1:6294539/2‑42 (MQ=16) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg < 1:4040841/48‑1 (MQ=25) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg < 1:1621867/48‑1 (MQ=25) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg < 1:4692585/48‑1 (MQ=25) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaagagg > 1:3129186/2‑69 (MQ=255) accacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTaa > 1:6637091/1‑59 (MQ=255) accacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaagagg < 1:4546941/70‑3 (MQ=255) accacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc < 1:4151007/71‑1 (MQ=255) ccacTACCGCCGTGGCGCTGGCGGATCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCa < 1:5470260/71‑1 (MQ=255) ccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGc < 1:5545807/62‑1 (MQ=255) ccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaagagg > 1:4185384/1‑67 (MQ=255) ccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc > 1:5945727/1‑70 (MQ=255) cactACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:7888624/71‑1 (MQ=255) cactACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:2034067/71‑1 (MQ=255) actaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaa < 1:5582654/68‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:2184884/69‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:919819/69‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:6433775/69‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa > 1:4799480/3‑69 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa > 1:6701361/3‑69 (MQ=255) taCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCttt < 1:5973533/51‑1 (MQ=37) taCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa > 1:4752525/2‑68 (MQ=255) aCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa > 1:7438602/1‑69 (MQ=255) ccgccgTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTaa > 1:1097965/1‑52 (MQ=37) cGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGc < 1:7795520/46‑1 (MQ=25) cGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTaa < 1:2856304/51‑1 (MQ=37) cGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa < 1:4701630/58‑1 (MQ=255) cGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa < 1:3800974/58‑1 (MQ=255) cGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaag < 1:5910025/59‑1 (MQ=255) cGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGAcc < 1:5518582/71‑1 (MQ=255) gccgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCa > 1:72968/4‑71 (MQ=255) ccgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTaa > 1:3452781/3‑49 (MQ=37) ccgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCAc > 1:7585875/3‑71 (MQ=255) ccgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCAc > 1:7249427/3‑71 (MQ=255) tgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGt < 1:3660262/71‑1 (MQ=255) cgcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGaa < 1:2612353/69‑1 (MQ=255) gcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACgg > 1:7616592/2‑66 (MQ=255) gcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAg > 1:3183348/2‑71 (MQ=255) ggCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaaga > 1:6966821/1‑48 (MQ=255) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:2442300/52‑1 (MQ=255) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcgg > 1:1143966/1‑71 (MQ=255) gAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCa < 1:151082/70‑1 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaagagg > 1:3487897/1‑43 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaagagg > 1:7175297/1‑43 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAgg > 1:3823134/1‑64 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAAt > 1:4462122/1‑71 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAAt < 1:5008932/71‑1 (MQ=255) gCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACggg < 1:512670/58‑2 (MQ=255) gCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGaa > 1:2270195/1‑61 (MQ=255) gCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATc < 1:1839314/71‑1 (MQ=255) catcatGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCAc < 1:1682562/54‑1 (MQ=255) catcatGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCt < 1:2105422/71‑1 (MQ=255) tCATGACGTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggc < 1:7379329/61‑1 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:7348104/46‑1 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:5443309/46‑1 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:693186/44‑1 (MQ=38) | TTGGCCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAAGAAGCAAAAGACCACGGTGAAGGCGGCAATCT > AP009048/3824849‑3824978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |