| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,824,919 | G→T | 100% | L91L (CTG→CTT) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,824,919 | 0 | G | T | 100.0% | 319.5 / NA | 106 | L91L (CTG→CTT) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (46/60); total (46/60) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 140 total aligned reads displayed.
GCCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAAGAAGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTA > AP009048/3824852‑3824986 | gCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgac < 1:2612917/71‑1 (MQ=255)gCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgac < 1:6096595/71‑1 (MQ=255) ccTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct < 1:3231018/71‑1 (MQ=255) cTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctg > 1:3735880/1‑71 (MQ=255) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct > 1:5172867/1‑69 (MQ=255) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctga > 1:7863963/1‑70 (MQ=255) gCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctgac > 1:5771561/1‑69 (MQ=255) gCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGcc > 1:5187653/1‑71 (MQ=255) gCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGc > 1:6753898/1‑70 (MQ=255) cTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGcc < 1:4412129/70‑1 (MQ=255) cTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGcc < 1:1323528/70‑1 (MQ=255) ttACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg > 1:3578159/1‑70 (MQ=255) tACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTa > 1:6568033/1‑71 (MQ=255) aCAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTAc > 1:2751745/1‑71 (MQ=255) cAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacc < 1:6556550/62‑1 (MQ=255) aaaGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTg > 1:4233641/1‑71 (MQ=255) ggATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCttt < 1:2743880/71‑1 (MQ=255) ggATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCttt < 1:6024241/71‑1 (MQ=255) gATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTa < 1:4548058/71‑1 (MQ=255) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct > 1:2182999/1‑53 (MQ=25) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct > 1:18395/1‑53 (MQ=25) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct > 1:314234/1‑53 (MQ=25) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgaccttgacct > 1:3075587/1‑53 (MQ=25) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAAcc < 1:4300060/70‑1 (MQ=255) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCg > 1:5451746/1‑71 (MQ=255) cGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt > 1:6355198/1‑63 (MQ=255) gATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACt < 1:6144929/59‑1 (MQ=255) gATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCa < 1:309930/71‑1 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTg > 1:7086049/1‑59 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTg > 1:574762/1‑59 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTg > 1:937073/1‑59 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCa > 1:7111877/1‑70 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctg < 1:2438601/50‑1 (MQ=19) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt < 1:2963052/60‑1 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt < 1:5684386/60‑1 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa < 1:2633275/71‑1 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa < 1:2375512/71‑1 (MQ=255) gcgaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTTACCGCa < 1:15634/64‑1 (MQ=255) gcgaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa > 1:3020275/4‑69 (MQ=255) cgaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaagag < 1:6078481/69‑2 (MQ=255) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATgaccttga > 1:6294539/2‑42 (MQ=16) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATgaccttga > 1:7031577/2‑42 (MQ=16) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATgaccttga > 1:166434/2‑42 (MQ=16) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATgaccttga > 1:1739204/2‑42 (MQ=16) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg < 1:1621867/48‑1 (MQ=25) accacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc < 1:4151007/71‑1 (MQ=255) accacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc < 1:1263178/71‑1 (MQ=255) ccacTACCGCCGTGGCGCTGGCGGATCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCa < 1:5470260/71‑1 (MQ=255) ccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTAc > 1:2438393/1‑50 (MQ=25) ccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGc < 1:5545807/62‑1 (MQ=255) ccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaagagg > 1:4185384/1‑67 (MQ=255) ccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc > 1:5945727/1‑70 (MQ=255) cactACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:2034067/71‑1 (MQ=255) cactACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:4634213/71‑1 (MQ=255) cactACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:4097911/71‑1 (MQ=255) actaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaa < 1:5582654/68‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:919819/69‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:2184884/69‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:6433775/69‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa > 1:6701361/3‑69 (MQ=255) taCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCttt < 1:5973533/51‑1 (MQ=37) taCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCttt < 1:1885410/51‑1 (MQ=37) taCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa > 1:4752525/2‑68 (MQ=255) taCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa > 1:7507720/2‑68 (MQ=255) aCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaagag > 1:7300140/1‑62 (MQ=255) ccgccgTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTaa > 1:1097965/1‑52 (MQ=37) cGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGc < 1:7795520/46‑1 (MQ=25) cGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTaa < 1:2856304/51‑1 (MQ=37) cGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa < 1:4701630/58‑1 (MQ=255) cGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa < 1:3800974/58‑1 (MQ=255) cGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGAcc < 1:5518582/71‑1 (MQ=255) gccgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCa > 1:3013685/4‑71 (MQ=255) ccgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTaa > 1:3452781/3‑49 (MQ=37) ccgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCAc > 1:7249427/3‑71 (MQ=255) cgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa > 1:4429544/2‑64 (MQ=255) tgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGt < 1:3660262/71‑1 (MQ=255) cgcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGaa < 1:2612353/69‑1 (MQ=255) gcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACgg > 1:7616592/2‑66 (MQ=255) gcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAg > 1:3183348/2‑71 (MQ=255) ggCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaaga > 1:6966821/1‑48 (MQ=255) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcgg > 1:1143966/1‑71 (MQ=255) ggAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaag < 1:1712235/44‑1 (MQ=38) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaagagg > 1:3487897/1‑43 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaagagg > 1:7175297/1‑43 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAgg > 1:3823134/1‑64 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAAt > 1:4462122/1‑71 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAAt < 1:5008932/71‑1 (MQ=255) gCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACggg < 1:512670/58‑2 (MQ=255) tCATGACGTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggc < 1:7379329/61‑1 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:7348104/46‑1 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:5443309/46‑1 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:693186/44‑1 (MQ=38) catgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggt > 1:6664949/4‑71 (MQ=255) atgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCaaaa > 1:283488/3‑44 (MQ=38) tgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttg > 1:5243238/2‑71 (MQ=255) tgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggt > 1:6135328/2‑69 (MQ=255) gaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttgg > 1:750048/1‑71 (MQ=255) accttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttgg > 1:1134962/1‑70 (MQ=255) accttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggtt > 1:5758100/1‑68 (MQ=255) ccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTa < 1:4770409/71‑1 (MQ=255) | GCCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAAGAAGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTA > AP009048/3824852‑3824986 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |