| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,824,949 | A→G | 100% | E101E (GAA→GAG) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,824,949 | 0 | A | G | 100.0% | 397.5 / NA | 113 | E101E (GAA→GAG) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (52/61); total (52/61) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 116 total aligned reads displayed.
ACCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAAGAAGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGT > AP009048/3824881‑3825014 | accacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc < 1:1263178/71‑1 (MQ=255)accacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc < 1:4151007/71‑1 (MQ=255) ccacTACCGCCGTGGCGCTGGCGGATCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCa < 1:5470260/71‑1 (MQ=255) ccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc > 1:5945727/1‑70 (MQ=255) cactACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:7888624/71‑1 (MQ=255) cactACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:4097911/71‑1 (MQ=255) cactACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:4634213/71‑1 (MQ=255) cactACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:5558287/71‑1 (MQ=255) cactACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:2034067/71‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:919819/69‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:2184884/69‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:6433775/69‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa > 1:4799480/3‑69 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa > 1:6701361/3‑69 (MQ=255) taCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa > 1:7507720/2‑68 (MQ=255) cGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGAcc < 1:5518582/71‑1 (MQ=255) gccgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCa < 1:7023213/68‑1 (MQ=255) gccgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCa > 1:72968/4‑71 (MQ=255) gccgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCa > 1:3013685/4‑71 (MQ=255) ccgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCAc > 1:7249427/3‑71 (MQ=255) ccgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCAc > 1:7585875/3‑71 (MQ=255) cgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa > 1:4429544/2‑64 (MQ=255) tgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGt < 1:3660262/71‑1 (MQ=255) gcgcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTg > 1:3794474/4‑70 (MQ=255) cgcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGaa < 1:2612353/69‑1 (MQ=255) gcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACgg > 1:7616592/2‑66 (MQ=255) gcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAg > 1:3183348/2‑71 (MQ=255) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:2442300/52‑1 (MQ=255) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcgg > 1:1143966/1‑71 (MQ=255) gAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCa < 1:151082/70‑1 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAAt < 1:5008932/71‑1 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAAt > 1:4462122/1‑71 (MQ=255) gCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACggg < 1:512670/58‑2 (MQ=255) gCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGaa > 1:2270195/1‑61 (MQ=255) gCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATc < 1:1839314/71‑1 (MQ=255) catcatGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCAc < 1:1682562/54‑1 (MQ=255) catcatGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCt < 1:2105422/71‑1 (MQ=255) tCATGACGTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggc < 1:7379329/61‑1 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:7348104/46‑1 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:5443309/46‑1 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:693186/44‑1 (MQ=38) catgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggt > 1:6664949/4‑71 (MQ=255) atgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCaaaa > 1:283488/3‑44 (MQ=38) atgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCaaaa > 1:5457310/3‑44 (MQ=38) atgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCaaaa > 1:5743633/3‑44 (MQ=38) tgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcg > 1:5316073/2‑59 (MQ=255) tgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAg > 1:2936160/2‑56 (MQ=255) tgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttg > 1:5243238/2‑71 (MQ=255) tgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggt > 1:6135328/2‑69 (MQ=255) gaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttgg > 1:750048/1‑71 (MQ=255) accttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttgg > 1:1134962/1‑70 (MQ=255) accttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggtt > 1:5758100/1‑68 (MQ=255) ccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACg > 1:3518765/1‑47 (MQ=255) ccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTa < 1:4770409/71‑1 (MQ=255) ttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGaa > 1:1749098/3‑50 (MQ=255) tgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGt > 1:7705254/2‑46 (MQ=255) tgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGt > 1:5709772/2‑46 (MQ=255) gacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtgg > 1:4557157/1‑61 (MQ=255) acctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGcgcg > 1:3258293/1‑71 (MQ=255) ctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAAt < 1:5881334/54‑1 (MQ=255) ctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAAt < 1:5871757/54‑1 (MQ=255) ccGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTa < 1:4652985/69‑1 (MQ=255) ccGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTAc < 1:6187060/70‑1 (MQ=255) ccGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTAc < 1:3072325/70‑1 (MQ=255) ccGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTAc < 1:2159141/70‑1 (MQ=255) cGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTAc > 1:3785937/1‑69 (MQ=255) cGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTAc > 1:1818895/1‑69 (MQ=255) gTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtgg > 1:1478025/1‑53 (MQ=255) tACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTg < 1:4313927/37‑1 (MQ=255) aCTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtg < 1:7507477/50‑1 (MQ=255) aCTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAgg > 1:6607191/1‑70 (MQ=255) cTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGa < 1:3877648/36‑1 (MQ=255) cTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGa > 1:7768462/1‑36 (MQ=255) cTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAgg > 1:7624649/1‑69 (MQ=255) gCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACaa > 1:2754374/1‑65 (MQ=255) cTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtg < 1:616296/46‑1 (MQ=255) cTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGAcgcg < 1:6324140/71‑1 (MQ=255) cTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGAcgc < 1:1204231/70‑1 (MQ=255) tttAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGAcg < 1:1945820/68‑1 (MQ=255) tttAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGAcg < 1:803770/68‑1 (MQ=255) ttAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttggt < 1:831748/50‑1 (MQ=255) ttAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGt > 1:5045834/1‑70 (MQ=255) tAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACa < 1:4296081/60‑1 (MQ=255) tAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACa < 1:456495/60‑1 (MQ=255) aaCCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACa < 1:7246440/59‑1 (MQ=255) ccGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggtt > 1:715476/1‑43 (MQ=255) ccGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggtt > 1:3104950/1‑43 (MQ=255) ccGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTaa > 1:3103976/1‑68 (MQ=255) ccGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGc < 1:1975375/71‑1 (MQ=255) ccGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGc < 1:4249472/71‑1 (MQ=255) cGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCt < 1:1336211/71‑1 (MQ=255) gCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTg > 1:167499/1‑71 (MQ=255) cAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATc < 1:3426539/35‑1 (MQ=255) cAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTg < 1:3417455/70‑1 (MQ=255) cAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTg < 1:4766084/70‑1 (MQ=255) cAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTg < 1:3142571/70‑1 (MQ=255) aaaGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGt < 1:6394351/71‑1 (MQ=255) aaaGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTAGt < 1:7064151/71‑1 (MQ=255) agaggCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTgg > 1:116151/1‑68 (MQ=255) agaggCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTgg > 1:6656302/1‑68 (MQ=255) | ACCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAAGAAGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGT > AP009048/3824881‑3825014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |