| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 226,300 | T→C | 5.1% | noncoding (542/2904 nt) | rrlH → | 23S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 226,300 | 0 | T | C | 5.1% | 392.5 / 7.4 | 137 | noncoding (542/2904 nt) | rrlH | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base T (68/62); new base C (3/4); total (71/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 143 total aligned reads displayed.
GGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCG > AP009048/226231‑226370 | ggCGAAAAGAACCCCGGCCAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATg < 1:2069943/71‑1 (MQ=17) gCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGc < 1:5805552/71‑1 (MQ=34) cGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCt < 1:6343241/71‑1 (MQ=34) gAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCt > 1:1933621/1‑70 (MQ=35) aaaGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAg > 1:7896166/1‑71 (MQ=34) aaGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCt > 1:4174558/1‑67 (MQ=35) aaGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAgg > 1:2621903/1‑71 (MQ=34) ccccGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCgtg < 1:363367/70‑1 (MQ=35) cccGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCAt < 1:5004856/58‑1 (MQ=31) ggCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACt > 1:6392815/1‑71 (MQ=34) cGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGc > 1:7715246/1‑71 (MQ=34) ggAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTAcc < 1:7795138/71‑1 (MQ=34) gAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCt > 1:234955/1‑71 (MQ=34) gAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCt > 1:7462528/1‑71 (MQ=34) aGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCtt < 1:664744/71‑1 (MQ=34) gTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCttt > 1:3389556/1‑71 (MQ=34) tGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCtttt > 1:1016332/1‑71 (MQ=34) tGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCtt < 1:4857287/69‑1 (MQ=35) tGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCtt < 1:1040391/69‑1 (MQ=35) tGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCG > 1:6714369‑M2/1‑53 (MQ=255) aaaaaGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTa > 1:1010816/1‑47 (MQ=38) aaaaaGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTa > 1:5203070/1‑47 (MQ=38) aaaaaGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTg > 1:6551940/1‑70 (MQ=35) aaaaGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAg < 1:2317061/47‑1 (MQ=38) aaaaGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAg < 1:5585895/47‑1 (MQ=38) aaaaGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAg < 1:1815121/47‑1 (MQ=38) aaaGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGta > 1:2795673/1‑70 (MQ=35) aaaGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGta > 1:6989932/1‑70 (MQ=35) aaGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGa < 1:4311353/53‑1 (MQ=35) aaGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGa > 1:7526771/1‑53 (MQ=35) aaGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGC < 1:2074707‑M2/47‑1 (MQ=255) aGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTa > 1:4083065/1‑43 (MQ=38) aGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGa > 1:4533717/1‑52 (MQ=35) aGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGa > 1:3382218/1‑52 (MQ=35) aGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATaa > 1:3864628/1‑71 (MQ=34) aGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATaa > 1:6364523/1‑71 (MQ=34) aGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATaa < 1:6998833/71‑1 (MQ=34) aCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCt > 1:4978433/1‑38 (MQ=40) aCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATgg > 1:2271276/1‑71 (MQ=34) aCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACATTTTGta > 1:5708179/1‑65 (MQ=25) ccTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTa < 1:3760066/39‑1 (MQ=40) ccTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTa < 1:3088307/39‑1 (MQ=40) cTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGC > 1:528305‑M2/1‑41 (MQ=255) cTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGC > 1:4549344‑M2/1‑41 (MQ=255) cTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGC > 1:7152365‑M2/1‑41 (MQ=255) cTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGC > 1:844986‑M2/1‑41 (MQ=255) cTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGC > 1:1949896‑M2/1‑41 (MQ=255) tGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACt < 1:2829785/48‑1 (MQ=38) tGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTg > 1:5018490/1‑60 (MQ=35) tGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATggg > 1:6809811/1‑69 (MQ=35) aaaCCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCttt > 1:4179325/1‑56 (MQ=35) aaaCCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTc < 1:513008/69‑1 (MQ=35) aaCCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGc < 1:1310610/71‑1 (MQ=34) aaCCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGc < 1:2629430/71‑1 (MQ=34) aaCCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGc < 1:3635753/71‑1 (MQ=34) aaCCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGc > 1:740508/1‑71 (MQ=34) ccGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTc < 1:1763792/66‑1 (MQ=35) ccGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTc < 1:4107163/66‑1 (MQ=35) gtacgtacAAGCAGTGGGAGCACGCTTAGGCGTacacggtttcaggttctttttcactcccctcgccgg > 1:5017502‑M2/1‑33 (MQ=255) gtacgtacAAGCAGTGGGAGCACGCTTAGGCGTacacggtttcaggttctttttcactccactcgcc < 1:409489‑M2/67‑35 (MQ=255) acgtacAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTtata > 1:2151637/1‑71 (MQ=34) cgtacAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTtata < 1:3163905/70‑1 (MQ=35) cgtacAAGCAGTGGGAGCATGCTTAGCCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTtata < 1:794234/70‑1 (MQ=25) gtacAAGCAGTGGGAGCATGCTTAGGCGTacacggtttcaggttctttttcactcccctcgccggggttct < 1:7452879‑M2/71‑43 (MQ=255) gtacAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCttt > 1:5324795/1‑45 (MQ=38) gtacAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATAtt > 1:1106597/1‑71 (MQ=34) gtacAAGCAGTGGGAGCACGCTTAGGCGTacacggtttcaggttctttttcactcccctcgcc > 1:4352168‑M2/1‑29 (MQ=255) tacAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAg < 1:7358497/59‑1 (MQ=35) tacAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAg < 1:7852256/59‑1 (MQ=35) tacAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAg < 1:6529289/59‑1 (MQ=35) acAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGta > 1:6609759/1‑47 (MQ=38) acAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCt > 1:425481/1‑71 (MQ=34) acAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCt > 1:434832/1‑71 (MQ=34) acAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCt > 1:2545282/1‑71 (MQ=34) cAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCt < 1:661792/70‑1 (MQ=35) cAAGCAGTGGGAGCACGCTTGTACGTacacggtttcaggttctttttcactcccctcgccggggttc < 1:2394356‑M2/67‑42 (MQ=255) aaGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTg > 1:748124/1‑70 (MQ=35) aaGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTg > 1:6346096/1‑70 (MQ=35) aaGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGt < 1:3492039/71‑1 (MQ=34) gCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATaa > 1:696232/1‑46 (MQ=38) gCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAg > 1:566552/1‑71 (MQ=34) aGTGGGAGCATGCTTAGGCGTacacggtttcaggttctttttcactcccctcgccggg < 1:3922802‑M2/58‑38 (MQ=255) aGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCa < 1:6707024/71‑1 (MQ=34) tGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCaa < 1:6133785/70‑1 (MQ=35) tGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAg > 1:3568052/1‑71 (MQ=34) ggAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTta < 1:4392620/55‑1 (MQ=35) ggAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGt > 1:5912354/1‑71 (MQ=34) ggAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGt > 1:1472728/1‑71 (MQ=34) aGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTa < 1:2159529/71‑1 (MQ=34) gCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTaa < 1:2464687/71‑1 (MQ=34) cATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGc < 1:3777794/45‑1 (MQ=38) cATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGc < 1:1725804/45‑1 (MQ=38) cATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCt < 1:2376826/57‑1 (MQ=35) aTGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTtata < 1:5961458/52‑1 (MQ=33) aTGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTtata < 1:3326394/52‑1 (MQ=33) tGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGc < 1:764172/43‑1 (MQ=32) tGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGc < 1:1464785/43‑1 (MQ=32) tGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAAcc > 1:3407065/1‑70 (MQ=31) tGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCg < 1:2513160/71‑1 (MQ=31) tGCTTAGGCCTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAAcc > 1:6518601/1‑70 (MQ=14) | GGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCG > AP009048/226231‑226370 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |