| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,825,051 | G→A | 100% | E135E (GAG→GAA) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,825,051 | 0 | G | A | 100.0% | 331.5 / NA | 111 | E135E (GAG→GAA) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (45/66); total (45/66) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 111 total aligned reads displayed.
GGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAGTCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAG > AP009048/3824983‑3825117 | ggtAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATc > 1:1553501/1‑71 (MQ=255) gtAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATc < 1:1876396/70‑1 (MQ=255) tAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGa < 1:6286794/71‑1 (MQ=255) gcgcGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGAt < 1:3761688/70‑1 (MQ=255) gcgcGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGAt < 1:7555156/70‑1 (MQ=255) gcgcGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATg < 1:1066503/71‑1 (MQ=255) gTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGgaga < 1:444967/71‑1 (MQ=255) ttACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGAt < 1:4149135/65‑1 (MQ=255) ttACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATgg < 1:1339233/67‑1 (MQ=255) tACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGat > 1:480159/1‑70 (MQ=255) aCAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGatta > 1:5470721/1‑71 (MQ=255) aGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGattatt < 1:3001918/70‑1 (MQ=255) aGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTc < 1:3258637/71‑1 (MQ=255) aGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTc < 1:5121780/71‑1 (MQ=255) aGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTc < 1:5579526/71‑1 (MQ=255) gACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAg < 1:6761424/71‑1 (MQ=255) aCGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAgg > 1:6288733/1‑71 (MQ=255) cgcgTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGa > 1:4222431/1‑55 (MQ=255) cgTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGct > 1:6926563/1‑69 (MQ=255) cgTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGct > 1:7727211/1‑69 (MQ=255) gTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAgg > 1:2055702/1‑67 (MQ=255) gTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa < 1:6282804/71‑1 (MQ=255) gTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa < 1:4785340/71‑1 (MQ=255) tAATTCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGAt < 1:4817860/52‑1 (MQ=255) tAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGAt < 1:1538750/52‑1 (MQ=255) tAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAAt < 1:7784833/71‑1 (MQ=255) aaTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATg > 1:2030488/1‑71 (MQ=255) aaTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATg > 1:26930/1‑71 (MQ=255) aTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATgg > 1:7648557/1‑71 (MQ=255) aTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATg > 1:365609/1‑70 (MQ=255) tGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGa < 1:4344280/48‑1 (MQ=255) tGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGa < 1:1800035/48‑1 (MQ=255) tGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGa < 1:1641696/48‑1 (MQ=255) gCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcg > 1:7114799/1‑71 (MQ=255) gCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcg > 1:2391000/1‑71 (MQ=255) cTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcg < 1:4432314/70‑1 (MQ=255) cTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcg < 1:2561234/70‑1 (MQ=255) tGGTAGATGATGTGGTCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcgc > 1:3130425/1‑70 (MQ=255) tGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATc < 1:1538004/43‑1 (MQ=255) gTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGcgcg < 1:5179997/69‑1 (MQ=255) tAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCg > 1:7606935/1‑41 (MQ=255) tAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGAc < 1:5261663/70‑1 (MQ=255) aGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATg < 1:2426835/43‑1 (MQ=255) aGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAgg > 1:7825941/1‑56 (MQ=255) aGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGc > 1:6434619/1‑71 (MQ=255) aGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGc > 1:4901962/1‑71 (MQ=255) gatgatGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa > 1:3147875/1‑59 (MQ=255) tgatgTGATCACCGCCGGAACGGCGATTCTCGAATCGATGGAGATTATTCAGGCTAAt < 1:2671265/58‑1 (MQ=255) tgatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACg < 1:1194095/67‑1 (MQ=255) tgatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTg > 1:7870506/1‑71 (MQ=255) gatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTc > 1:6886789/1‑49 (MQ=255) gatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTg < 1:7503314/70‑1 (MQ=255) atgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCa < 1:2180801/49‑1 (MQ=255) atgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAg < 1:7055347/50‑1 (MQ=255) atgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGc < 1:295193/70‑1 (MQ=255) atgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGc < 1:7571131/70‑1 (MQ=255) gtgATCACCGCCGGAACGGCGCTTCGCGAATCGATGGAGATTATTCAGGCTAATGGcgcgc > 1:3611878/1‑60 (MQ=255) tgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGct < 1:3997054/50‑2 (MQ=39) gATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgc < 1:7336396/55‑1 (MQ=255) gATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgc < 1:297207/55‑1 (MQ=255) aTCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGct < 1:5800934/48‑2 (MQ=39) aTCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGct < 1:3080618/48‑2 (MQ=39) aTCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTg < 1:234587/64‑1 (MQ=255) aTCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgt < 1:4082365/71‑1 (MQ=255) tCACCGCCGGAACGGCGATTCGCGAATCGATGGAGattat < 1:1605894/40‑1 (MQ=255) tCACCGCCGGAACGGCGATTCGCGAATCGATGGAGattat < 1:4170209/40‑1 (MQ=255) cACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCt > 1:504949/1‑64 (MQ=255) cACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat > 1:1299631/1‑69 (MQ=255) cACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat > 1:2268702/1‑69 (MQ=255) aCCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTAtt < 1:5728880/71‑1 (MQ=255) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTg > 1:7361761/1‑57 (MQ=255) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGc < 1:3063206/58‑1 (MQ=255) cgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat < 1:3156961/65‑3 (MQ=255) cgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAttt > 1:7100908/1‑71 (MQ=255) cgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt > 1:4227228/1‑70 (MQ=255) ggAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGAGGCTTGCTGGCGTATTGATTTc < 1:3742956/71‑1 (MQ=255) ggAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCt > 1:1876256/1‑53 (MQ=39) aaCGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGc < 1:1803908/71‑1 (MQ=255) aaCGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGc < 1:6605633/71‑1 (MQ=255) cGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt > 1:1066493/1‑70 (MQ=255) ggCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTc < 1:6915248/66‑1 (MQ=255) ggCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCttg < 1:4503422/71‑3 (MQ=255) ggCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCttg < 1:4019174/71‑3 (MQ=255) gCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCttg < 1:5605231/70‑3 (MQ=255) cGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGa > 1:311305/1‑70 (MQ=255) cGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGAt > 1:6975432/1‑71 (MQ=255) cGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGAt > 1:5169266/1‑71 (MQ=255) cGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCGGGCGTAtt > 1:6523140/1‑58 (MQ=255) gATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat < 1:584479/56‑3 (MQ=255) gATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat < 1:2385735/56‑3 (MQ=255) gATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGa < 1:2778039/59‑1 (MQ=255) aTTCGCGAATCGATGGAGATTATTCAGGCTAATGgc < 1:5992511/36‑1 (MQ=37) aTTCGCGAATCGATGGAGATTATTCAGGCTAATGgc < 1:5630287/36‑1 (MQ=37) aTTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATc > 1:3558396/1‑70 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTc > 1:59857/1‑60 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt > 1:1835380/1‑63 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt > 1:4473423/1‑63 (MQ=255) cgcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCttg < 1:372328/64‑3 (MQ=255) cgcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt > 1:6563619/1‑69 (MQ=255) gcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAg < 1:782192/71‑1 (MQ=255) | GGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAGTCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAG > AP009048/3824983‑3825117 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |