| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,825,072 | C→T | 100% | A142A (GCC→GCT) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,825,072 | 0 | C | T | 100.0% | 363.3 / NA | 120 | A142A (GCC→GCT) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (53/67); total (53/67) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 120 total aligned reads displayed.
GTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAGTCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGGAACGCGGGCGCGGCGAGATTT > AP009048/3825004‑3825139 | gTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa > 1:4102933/1‑71 (MQ=255) tAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAAt < 1:7784833/71‑1 (MQ=255) aaTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATg > 1:26930/1‑71 (MQ=255) aaTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATg > 1:6141976/1‑71 (MQ=255) aTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATgg > 1:7648557/1‑71 (MQ=255) aTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATg > 1:365609/1‑70 (MQ=255) gCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcg > 1:7114799/1‑71 (MQ=255) cTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcg < 1:2561234/70‑1 (MQ=255) cTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcg < 1:4432314/70‑1 (MQ=255) tGGTAGATGATGTGGTCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcgc > 1:3130425/1‑70 (MQ=255) gTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGcgcg < 1:5179997/69‑1 (MQ=255) tAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGAc < 1:5261663/70‑1 (MQ=255) aGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGc > 1:4901962/1‑71 (MQ=255) aGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGc > 1:6434619/1‑71 (MQ=255) gatgatGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa > 1:3147875/1‑59 (MQ=255) tgatgTGATCACCGCCGGAACGGCGATTCTCGAATCGATGGAGATTATTCAGGCTAAt < 1:2671265/58‑1 (MQ=255) tgatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACg < 1:1194095/67‑1 (MQ=255) tgatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTg > 1:7870506/1‑71 (MQ=255) gatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGCCGCTTg > 1:4530168/1‑70 (MQ=255) atgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGc < 1:295193/70‑1 (MQ=255) atgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGc < 1:7571131/70‑1 (MQ=255) gtgATCACCGCCGGAACGGCGCTTCGCGAATCGATGGAGATTATTCAGGCTAATGGcgcgc > 1:3611878/1‑60 (MQ=255) gATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgc < 1:7336396/55‑1 (MQ=255) gATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgc < 1:297207/55‑1 (MQ=255) aTCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTg < 1:234587/64‑1 (MQ=255) cACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa > 1:6908543/1‑48 (MQ=38) cACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCt > 1:504949/1‑64 (MQ=255) cACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat > 1:2268702/1‑69 (MQ=255) cACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat > 1:1299631/1‑69 (MQ=255) aCCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTAtt < 1:5728880/71‑1 (MQ=255) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTg > 1:7361761/1‑57 (MQ=255) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGc < 1:3063206/58‑1 (MQ=255) cgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat < 1:3156961/65‑3 (MQ=255) cgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt > 1:4227228/1‑70 (MQ=255) ggAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGAGGCTTGCTGGCGTATTGATTTc < 1:3742956/71‑1 (MQ=255) ggAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCt > 1:1876256/1‑53 (MQ=39) aaCGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGc < 1:2327582/71‑1 (MQ=255) aaCGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGc < 1:6605633/71‑1 (MQ=255) aaCGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGc < 1:1803908/71‑1 (MQ=255) cGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt > 1:1066493/1‑70 (MQ=255) ggCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCttg < 1:4503422/71‑3 (MQ=255) ggCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCttg < 1:4019174/71‑3 (MQ=255) gCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCttg < 1:5605231/70‑3 (MQ=255) cGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGAt > 1:6975432/1‑71 (MQ=255) cGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGAt > 1:5169266/1‑71 (MQ=255) cGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCGGGCGTAtt > 1:6523140/1‑58 (MQ=255) gATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat < 1:2385735/56‑3 (MQ=255) gATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat < 1:584479/56‑3 (MQ=255) aTTCGCGAATCGATGGAGATTATTCAGGCTAATGgc < 1:5992511/36‑1 (MQ=37) aTTCGCGAATCGATGGAGATTATTCAGGCTAATGgc < 1:5630287/36‑1 (MQ=37) aTTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCg < 1:526998/71‑1 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:7534149/71‑1 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTc > 1:59857/1‑60 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt > 1:1835380/1‑63 (MQ=255) cgcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCttg < 1:372328/64‑3 (MQ=255) cgcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCttg < 1:2964414/64‑3 (MQ=255) cgcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt > 1:6563619/1‑69 (MQ=255) gcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAg < 1:782192/71‑1 (MQ=255) gaatCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGa < 1:1355059/68‑1 (MQ=255) atCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCt > 1:2723881/2‑57 (MQ=255) tCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCttg < 1:2019938/58‑3 (MQ=255) tCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGAt > 1:5707806/1‑60 (MQ=255) tCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcg > 1:5370576/1‑71 (MQ=255) gATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcggg < 1:7069772/71‑3 (MQ=255) gATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcggg < 1:3374391/71‑3 (MQ=255) aTGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgta < 1:5951954/42‑2 (MQ=38) aTGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgta < 1:5369063/42‑2 (MQ=38) tgcagaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGgggg < 1:6495493/68‑1 (MQ=255) ggAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTAtt > 1:5823641/1‑42 (MQ=38) gagaTTATTCAGGCTAGTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgcg < 1:7508201/71‑1 (MQ=255) gagaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGc > 1:3997918/1‑49 (MQ=39) gagaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGc > 1:6715784/1‑49 (MQ=39) gagaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATc > 1:6287203/1‑55 (MQ=255) agaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgcgc < 1:3476906/71‑1 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt > 1:2898531/1‑48 (MQ=38) gaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGa > 1:5475235/1‑51 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcggg > 1:6991996/1‑63 (MQ=255) attattCAGGGTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcgg < 1:5128627/63‑2 (MQ=255) attattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt < 1:433017/42‑1 (MQ=38) attattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt < 1:4010147/42‑1 (MQ=38) attattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt < 1:1169622/42‑1 (MQ=38) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTg < 1:7302691/38‑1 (MQ=37) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt < 1:7601099/41‑1 (MQ=38) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt < 1:7609964/41‑1 (MQ=38) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt < 1:6957727/41‑1 (MQ=38) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt < 1:460887/41‑1 (MQ=38) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:357762/1‑71 (MQ=255) ttatcCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt < 1:7062323/41‑1 (MQ=37) attCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGc < 1:4475441/43‑1 (MQ=38) attCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGc < 1:1532098/43‑1 (MQ=38) attCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggcg < 1:2487428/70‑1 (MQ=255) ttgaGGCTATTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCga < 1:6790716/67‑1 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:4590875/50‑1 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCga < 1:6430320/70‑1 (MQ=255) cAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGAt > 1:2381877/1‑45 (MQ=38) cAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAt > 1:4139146/1‑71 (MQ=255) aGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGc < 1:4004857/39‑1 (MQ=37) aGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcg > 1:7772683/1‑55 (MQ=255) aGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAtt < 1:3833071/71‑1 (MQ=255) ggCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAttt < 1:7350767/71‑1 (MQ=255) | GTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAGTCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGGAACGCGGGCGCGGCGAGATTT > AP009048/3825004‑3825139 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |