| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,825,123 | C→G | 100% | R159R (CGC→CGG) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,825,123 | 0 | C | G | 99.0% | 249.0 / ‑6.0 | 72 | R159R (CGC→CGG) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (27/44); minor base T (1/0); total (28/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch. | |||||||||||
TGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGGAACGCGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTAT > AP009048/3825056‑3825188 | tgcagaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGgggg < 1:6495493/68‑1 (MQ=255) gagaTTATTCAGGCTAGTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgcg < 1:7508201/71‑1 (MQ=255) agaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgcgc < 1:3476906/71‑1 (MQ=255) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:357762/1‑71 (MQ=255) attCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggcg < 1:2487428/70‑1 (MQ=255) ttgaGGCTATTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCga < 1:6790716/67‑1 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCga < 1:6430320/70‑1 (MQ=255) tCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:5225201/1‑67 (MQ=255) cAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAt > 1:4139146/1‑71 (MQ=255) aGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAtt < 1:3833071/71‑1 (MQ=255) ggCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAttt < 1:7350767/71‑1 (MQ=255) gctaaTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTc < 1:4689352/68‑1 (MQ=255) gctaaTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTc < 1:2315815/68‑1 (MQ=255) aaTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGc > 1:3837055/1‑71 (MQ=255) aTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCgg < 1:2229671/69‑1 (MQ=255) aTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCgg < 1:2024813/69‑1 (MQ=255) tGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGa < 1:800517/71‑1 (MQ=255) gcgcGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGAtt < 1:7416865/71‑1 (MQ=255) gcgcGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGAtt < 1:7566626/71‑1 (MQ=255) cgcgACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTc < 1:6785786/71‑1 (MQ=255) cgcgACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGCCGATTc < 1:4525007/71‑1 (MQ=255) gcgACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCa < 1:7031708/71‑1 (MQ=255) aCGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGgggg < 1:3725865/45‑1 (MQ=38) gCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGTGGgcg > 1:918467/1‑45 (MQ=38) gCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAg > 1:848342/1‑71 (MQ=255) cTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGc < 1:5283355/59‑1 (MQ=255) cTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGc < 1:3750757/59‑1 (MQ=255) cTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGc < 1:5265666/59‑1 (MQ=255) gCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGtt < 1:7671517/69‑1 (MQ=255) cTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGa < 1:2018133/70‑1 (MQ=255) ggCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCg > 1:7003781/1‑71 (MQ=255) ggCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCg > 1:506524/1‑71 (MQ=255) gCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGtt < 1:1610088/65‑1 (MQ=255) gCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGc < 1:3917853/69‑1 (MQ=255) gCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGt < 1:6366694/71‑1 (MQ=255) gtattGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGa > 1:466300/4‑71 (MQ=255) tattGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTc < 1:3346246/44‑1 (MQ=38) attGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGAt > 1:3564878/2‑70 (MQ=255) ttGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGAtt < 1:4341040/70‑1 (MQ=255) ttGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTa < 1:6153803/71‑1 (MQ=255) tGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTa > 1:3099649/1‑70 (MQ=255) aTTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACaa < 1:1214513/71‑1 (MQ=255) aTTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACaa > 1:442962/1‑71 (MQ=255) aTTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACa < 1:6057039/70‑1 (MQ=255) tttCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAgg < 1:7902882/51‑1 (MQ=255) tCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGAtt > 1:4306547/1‑45 (MQ=255) cGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTg > 1:2452764/1‑70 (MQ=255) ttgATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCa > 1:7226851/3‑69 (MQ=255) tgATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGaa > 1:7514079/2‑46 (MQ=255) tgATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCaa < 1:1831210/68‑1 (MQ=255) tgATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAg > 1:669132/2‑71 (MQ=255) gATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAg > 1:7634300/1‑51 (MQ=255) gATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGa > 1:4041512/1‑56 (MQ=255) gATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGt > 1:6755176/1‑71 (MQ=255) gATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGt > 1:1618125/1‑71 (MQ=255) gATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGt > 1:1627321/1‑71 (MQ=255) aTCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTg < 1:435955/71‑1 (MQ=255) cGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGAt > 1:3531612/1‑71 (MQ=255) tCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGa < 1:3495287/45‑1 (MQ=255) cAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGa > 1:769484/1‑44 (MQ=255) cAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAg > 1:5448236/1‑45 (MQ=255) cAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAg > 1:2260655/1‑45 (MQ=255) aGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACt > 1:3576937/1‑57 (MQ=255) aGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACt > 1:2306142/1‑57 (MQ=255) aGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATctct < 1:7169939/71‑1 (MQ=255) ggAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTa < 1:6830450/71‑1 (MQ=255) gAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGa < 1:2212033/35‑1 (MQ=255) gAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGa < 1:3225603/35‑1 (MQ=255) gAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGa < 1:5129365/35‑1 (MQ=255) gAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGAtc < 1:7916849/66‑1 (MQ=255) gAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTat < 1:1462365/71‑1 (MQ=255) gAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTat < 1:1079423/71‑1 (MQ=255) aaCGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGt < 1:7561623/44‑1 (MQ=255) aCGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTa < 1:5970889/68‑1 (MQ=255) | TGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGGAACGCGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTAT > AP009048/3825056‑3825188 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |