| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,825,216 | C→T | 100% | Y190Y (TAC→TAT) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,825,216 | 0 | C | T | 100.0% | 274.4 / NA | 92 | Y190Y (TAC→TAT) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (40/52); total (40/52) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATCGCGAAGAGTTT > AP009048/3825150‑3825279 | ggAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTgg < 1:1550208/71‑1 (MQ=255)ggAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTg > 1:7330236/1‑70 (MQ=255) aaGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTgg > 1:6722452/1‑69 (MQ=255) aGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAag < 1:2094922/71‑1 (MQ=255) gTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTgg < 1:5848457/67‑1 (MQ=255) gTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGaa > 1:802478/1‑69 (MQ=255) gTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAaga < 1:3594807/71‑1 (MQ=255) gTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAaga < 1:3269838/71‑1 (MQ=255) gTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAaga < 1:251676/71‑1 (MQ=255) gTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAaga < 1:145593/71‑1 (MQ=255) ttGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAagag < 1:832222/71‑1 (MQ=255) tGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAagag > 1:7545473/1‑70 (MQ=255) gAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAaga > 1:404399/1‑68 (MQ=255) aGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAg > 1:2813475/1‑71 (MQ=255) gCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAgaga > 1:3147174/1‑68 (MQ=255) gCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGc < 1:786453/71‑1 (MQ=255) tGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGcc < 1:7353509/69‑1 (MQ=255) tGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGcc < 1:5879748/69‑1 (MQ=255) tGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCgg < 1:1606917/71‑1 (MQ=255) aTTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCgg > 1:7330531/1‑69 (MQ=255) aTTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCgg > 1:5849133/1‑69 (MQ=255) aTTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCgg > 1:7036496/1‑69 (MQ=255) aTTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGaa < 1:5783270/71‑1 (MQ=255) aTTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGaa < 1:5910878/71‑1 (MQ=255) aTTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGa < 1:5559905/70‑1 (MQ=255) tACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAAt > 1:3717963/1‑71 (MQ=255) aCAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAagag > 1:1338930/1‑59 (MQ=38) cAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATgg > 1:3776052/1‑71 (MQ=255) cAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATg < 1:4790229/70‑1 (MQ=255) cAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATg < 1:6926011/70‑1 (MQ=255) cAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATg < 1:987778/70‑1 (MQ=255) aaCTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGc < 1:554199/61‑1 (MQ=255) aaCTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGc < 1:1412897/71‑1 (MQ=255) cTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCg > 1:3510420/1‑70 (MQ=255) tGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCgg > 1:5284581/1‑70 (MQ=255) cAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCg < 1:7676805/67‑1 (MQ=255) cAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCg < 1:3258414/67‑1 (MQ=255) cAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGaa > 1:3668861/1‑70 (MQ=255) cAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGa < 1:2694892/69‑1 (MQ=255) cAAAGTGATCTCTATCATCACCCTGAAAGATATGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCgg > 1:6504479/1‑68 (MQ=255) aaaGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACa < 1:2316765/71‑1 (MQ=255) gTGATCTCTATCATCACCCTGAAAGATCTGATGGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCt > 1:6465145/1‑71 (MQ=255) tGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGc > 1:2667087/1‑51 (MQ=38) tGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGc > 1:5288726/1‑51 (MQ=38) tGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGc > 1:4897106/1‑51 (MQ=38) aTCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAAt < 1:1772639/56‑1 (MQ=38) tctATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGaa < 1:5017695/44‑1 (MQ=25) tctATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTggcggc > 1:3900297/1‑71 (MQ=255) ctATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGgcggcg > 1:2687971/1‑71 (MQ=255) tATCATCACCCTGAAAGATCTGATTGCCTATCTGGa < 1:6962896/36‑1 (MQ=21) tATCATCACCCTGAAAGATCTGATTGCCTATCTGGa < 1:2956585/36‑1 (MQ=21) tATCATCACCCTGAAAGATCTGATTGCCTATCTGGa < 1:3878447/36‑1 (MQ=21) tATCATCACCCTGAAAGATCTGATTGCCTATCTGGa < 1:5909923/36‑1 (MQ=21) tATCATCACCCTGAAAGATCTGATTGCCTATCTGGa < 1:5738160/36‑1 (MQ=21) tATCATCACCCTGAAAGATCTGATTGCCTATCTGGa < 1:1290945/36‑1 (MQ=21) tATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTggcggc < 1:2485798/69‑1 (MQ=39) tATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTggcggc < 1:2654715/69‑1 (MQ=39) tATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTggcggc < 1:7011387/69‑1 (MQ=39) tATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGcggcgg > 1:6820695/1‑71 (MQ=255) atcatcACCCTGAAAGATCTGATTGCCTATCTGGa < 1:6046047/35‑1 (MQ=21) atcatcACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAg > 1:2507891/1‑42 (MQ=25) atcatcACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAg > 1:5645982/1‑42 (MQ=25) tcatcaCCCTGAAAGATCTGATTGCCTATCTGTAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGtt < 1:2948899/71‑1 (MQ=255) catcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGc > 1:3481267/1‑51 (MQ=38) atcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGtt > 1:1660342/1‑69 (MQ=39) atcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTaa < 1:4819452/71‑1 (MQ=255) tcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAg < 1:5303332/71‑1 (MQ=255) tcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAg < 1:6820222/71‑1 (MQ=255) caCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAAt < 1:4128047/45‑1 (MQ=37) caCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTggcgg < 1:3603088/62‑1 (MQ=38) ccTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAAc < 1:2717707/50‑1 (MQ=38) cTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAgg < 1:3597620/67‑1 (MQ=39) tGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACAt > 1:3660055/1‑50 (MQ=38) tGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTAt > 1:5741753/1‑71 (MQ=255) tGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTAt > 1:7101017/1‑71 (MQ=255) agAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGCCGGTTaa > 1:5323970/2‑64 (MQ=255) gAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGtt < 1:371193/61‑1 (MQ=38) aaaGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAAc > 1:417316/1‑46 (MQ=37) aaaGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATcg > 1:7479453/1‑71 (MQ=255) aGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATcgcg < 1:1863989/71‑1 (MQ=255) aGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATcgcg < 1:6358385/71‑1 (MQ=255) gatcTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATcgcg < 1:5757150/67‑1 (MQ=255) tcTGATTGCCTATCTGGAAGAGAAGCCGGAAATTGCGGAACATCTggcg > 1:7514/2‑49 (MQ=37) tcTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGCCCTATcgcg < 1:1933089/67‑1 (MQ=38) gATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTa < 1:2029221/53‑1 (MQ=38) aTTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGcc > 1:2063292/1‑57 (MQ=38) aTTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGcc > 1:4939391/1‑57 (MQ=38) aTTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGcc > 1:5685929/1‑57 (MQ=38) aTTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATCGCGAAGAGtt < 1:2957130/71‑1 (MQ=255) ttGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATCGCGAAGAGttt < 1:6031585/71‑1 (MQ=255) tGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTaa > 1:4204105/1‑51 (MQ=38) tGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTaa > 1:1837806/1‑51 (MQ=38) | GGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATCGCGAAGAGTTT > AP009048/3825150‑3825279 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |