| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,985,588 | T→A | 5.4% | M49L (ATG→TTG) | yhiF ← | predicted DNA‑binding ranscriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,985,588 | 0 | T | A | 5.4% | 425.1 / 12.2 | 147 | M49L (ATG→TTG) | yhiF | predicted DNA‑binding ranscriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (69/70); new base A (5/3); total (74/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
Only 100 of 155 total aligned reads displayed.
AAAATAATGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTCTGTAT > AP009048/3985518‑3985654 | aaaaTAATGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCAt < 1:2505885/71‑1 (MQ=255) aataatGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCAtt > 1:4600485/1‑70 (MQ=255) taatGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTaa > 1:6379511/1‑70 (MQ=255) taatGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAAt < 1:6227926/71‑1 (MQ=255) taatGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAAt < 1:7170197/71‑1 (MQ=255) taatGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAAt > 1:330653/1‑71 (MQ=255) taatGACATGGACAGGTTTTAATAGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAAt > 1:6322599/1‑71 (MQ=255) atGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATg > 1:7256560/1‑70 (MQ=255) atGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATg > 1:1912963/1‑70 (MQ=255) atGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGt < 1:2000561/71‑1 (MQ=255) tGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGt > 1:3539366/1‑70 (MQ=255) cATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATc > 1:2614252/1‑70 (MQ=255) cATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCa < 1:4308808/71‑1 (MQ=255) tGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaat < 1:2852621/71‑1 (MQ=255) tGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaa < 1:1828089/70‑1 (MQ=255) aCAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTa > 1:501894/1‑58 (MQ=255) aCAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaataat < 1:6283109/71‑1 (MQ=255) aCAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaataat < 1:6107411/71‑1 (MQ=255) aCAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaataat < 1:4716354/71‑1 (MQ=255) aCAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaataat < 1:5314264/71‑1 (MQ=255) aGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTATTGATACATTAATGa < 1:3201127/71‑1 (MQ=37) ggTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGAc > 1:4307000/1‑71 (MQ=255) gTTTTAATCGTTCAATTTTAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaata < 1:5217415/65‑1 (MQ=255) gTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATTAATAATGAc > 1:5636271/1‑70 (MQ=255) gTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACg < 1:6327927/71‑1 (MQ=255) gTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACg < 1:6771886/71‑1 (MQ=255) ttttAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACg < 1:4667897/70‑1 (MQ=255) ttttAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGa < 1:5157708/71‑1 (MQ=255) ttttAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGa < 1:5148182/71‑1 (MQ=255) tAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaata < 1:903626/61‑1 (MQ=255) aTCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCa < 1:7301542/71‑1 (MQ=255) tCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCAt > 1:3079096/1‑71 (MQ=255) tCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCAt > 1:5919592/1‑71 (MQ=255) tCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCAt < 1:129538/71‑1 (MQ=255) gTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTc > 1:408861/1‑71 (MQ=255) tCAATTTGAGTGAGAAAATTAGAATGAAATACATAATTCAtt > 1:2144183/1‑42 (MQ=255) cAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTg < 1:5123216/70‑1 (MQ=255) aaTTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGa < 1:7844567/70‑1 (MQ=255) aaTTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGAt > 1:6369991/1‑71 (MQ=255) aTTTGAGTGAGAAAATTAGAATGAAATACATTATTCAtt < 1:4331105/39‑1 (MQ=255) aTTTGAGTGAGAAAATTAGAATGAAATACATTATTCAtt < 1:5352809/39‑1 (MQ=255) aTTTGAGTGAGAAAATTAGAATGAAATACATTATTCAtt < 1:82653/39‑1 (MQ=255) aTTTGAGTGAGAAAATTAGAATGAAATACATTATTCAtt < 1:1897330/39‑1 (MQ=255) aTTTGAGTGAGAAAATTAGAATGAAATACATTATTCAtt < 1:2114608/39‑1 (MQ=255) tttGAGTGAGAAAATTAGAATTAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGc < 1:5762774/71‑1 (MQ=255) tttGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGaa > 1:93267/1‑58 (MQ=255) tttGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGc < 1:4040635/71‑1 (MQ=255) ttGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaa > 1:3848210/1‑47 (MQ=255) ttGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaa > 1:6637949/1‑47 (MQ=255) tgagtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCaa > 1:1623621/1‑71 (MQ=255) gagtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCaa < 1:2940259/70‑1 (MQ=255) agtgagAAAATTAGAATGAAATACATTATTCATTAATGt < 1:5974578/39‑1 (MQ=255) agtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACa < 1:283733/71‑1 (MQ=255) agtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACa < 1:2951635/71‑1 (MQ=255) gtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCaat < 1:1850129/44‑1 (MQ=255) gtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCAtt > 1:3971425/1‑59 (MQ=255) gagaAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGa < 1:3637760/50‑1 (MQ=255) agaAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTa < 1:6857613/70‑1 (MQ=255) agaAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTAc < 1:5629336/71‑1 (MQ=255) agaAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTAc < 1:614123/71‑1 (MQ=255) gaAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCt < 1:1255461/57‑1 (MQ=255) aaaaTTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGa > 1:7864440/1‑58 (MQ=255) aaaaTTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGt > 1:2925153/1‑71 (MQ=255) aaTTAGAATGACATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCg < 1:273478/71‑1 (MQ=255) aaTTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGaa > 1:6826973/1‑46 (MQ=255) aaTTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGaa > 1:939367/1‑46 (MQ=255) ttAGAATGAAATACATTATTCATTAATGTATCAATAATGACGa > 1:6748605/1‑43 (MQ=255) ttAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCTTTCTGATGCAACATTACGTCg > 1:5115844/1‑69 (MQ=255) ttAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGAt < 1:4387147/71‑1 (MQ=255) gAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGa > 1:5767621/1‑51 (MQ=255) aaTGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATctct < 1:5368297/71‑1 (MQ=255) tGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGAt > 1:1722611/1‑65 (MQ=255) tGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGAt > 1:5228206/1‑65 (MQ=255) tGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCtcttc > 1:1135501/1‑71 (MQ=255) tgaTACATTAATGAATAATGTATTAATAATGACGAAGGCATTCTGATGCAACATTACGTCGAtctc > 1:1537633/3‑66 (MQ=25) tgaTACATTAATGAATAATGTATTAATAATGACGAAGGCATTCTGATGCAACATTACGTCGAtctc > 1:2393729/3‑66 (MQ=25) tgaTACATTAATGAATAATGTATTAATAATGACGAAGGCATTCTGATGCAACATTACGTCGAtctc > 1:3027315/3‑66 (MQ=25) aaaTACATTATTCATTAATGTATCAATAATGACGAAGGCAt < 1:3125217/41‑1 (MQ=255) aaaTACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTAc < 1:5469590/57‑1 (MQ=255) gaTACATTAATTAATAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTAc < 1:7163777/55‑1 (MQ=255) gaTACATTAATGAATAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTAc < 1:2383549/55‑1 (MQ=37) aaTACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCttcttc > 1:7549727/1‑71 (MQ=255) aTACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCg > 1:5384949/1‑71 (MQ=255) aTACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCg < 1:4009303/71‑1 (MQ=255) aTACATTAATGAATAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCtcttc < 1:890980/67‑1 (MQ=38) tACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGt < 1:426062/71‑1 (MQ=255) aCATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGtt > 1:6996859/1‑71 (MQ=255) aCATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGt > 1:955632/1‑70 (MQ=255) aCATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGt > 1:389942/1‑70 (MQ=255) cATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGAt < 1:3768597/42‑1 (MQ=255) cATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGAt < 1:3490887/42‑1 (MQ=255) cATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTc < 1:3851976/55‑1 (MQ=255) cATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTc < 1:3716232/55‑1 (MQ=255) cATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTc > 1:7022627/1‑71 (MQ=255) tattCATTAATGTATCAATAATGACGAAGGCATTCt < 1:2200689/36‑1 (MQ=255) tattCATTAATGTATCAATAATGACGAAGGCATTCTg > 1:7644928/1‑37 (MQ=255) ttCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTCTGtat < 1:3910078/71‑1 (MQ=255) ttCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTCTGt < 1:5962736/69‑1 (MQ=255) cATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGAtctc < 1:5114191/54‑1 (MQ=255) aTTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCtctt > 1:2249321/1‑55 (MQ=255) | AAAATAATGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTCTGTAT > AP009048/3985518‑3985654 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |