| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 4,070,984 | T→C | 5.9% | I630T (ATT→ACT) | glgX → | glycogen debranching enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 4,070,984 | 0 | T | C | 5.9% | 289.7 / 7.7 | 102 | I630T (ATT→ACT) | glgX | glycogen debranching enzyme |
| Reads supporting (aligned to +/- strand): ref base T (47/49); new base C (4/2); total (51/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
TTTTGATCGCAATTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGACCTGCACACGGATTGTG > AP009048/4070915‑4071053 | ttttGATCGCAATTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCAtt > 1:7278759/1‑71 (MQ=255)ttttGATCGCAATTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCAtt > 1:6529780/1‑71 (MQ=255)ttttGATCGCAATTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCAtt > 1:6676174/1‑71 (MQ=255)ttttGATCGCAATTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCAtt > 1:2845256/1‑71 (MQ=255) tttGATCGCAATTAACGCCATGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTc > 1:2582300/1‑71 (MQ=255) tttGATCGCAATTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCAt < 1:7930430/69‑1 (MQ=255) tttGATCGCAATTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTc > 1:2274432/1‑71 (MQ=255) tttGATCGCAATTAACG‑‑ACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTccc > 1:6128497/1‑71 (MQ=255) ttGATCGCAATTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTcc > 1:7018638/1‑71 (MQ=255) aTCGCAATTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTcccc > 1:3896118/1‑70 (MQ=255) cGCAATTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCAt > 1:5460491/1‑71 (MQ=255) gCAATTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCAtt < 1:6210915/71‑1 (MQ=255) gCAATTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCAtt > 1:3363929/1‑71 (MQ=255) gCAATTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCAtt < 1:2154538/71‑1 (MQ=255) gCAATTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCAtt > 1:2155683/1‑71 (MQ=255) aaTTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCg > 1:934418/1‑71 (MQ=255) aaTTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCg > 1:1521085/1‑71 (MQ=255) aaTTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCg > 1:2782101/1‑71 (MQ=255) aaTTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCg > 1:6269138/1‑71 (MQ=255) aaTTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCg > 1:2317453/1‑71 (MQ=255) aTTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTc < 1:5345761/61‑1 (MQ=255) aTTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGc > 1:1384798/1‑71 (MQ=255) aTTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGc > 1:6793701/1‑71 (MQ=255) aTTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGc > 1:1586168/1‑71 (MQ=255) ttAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCt > 1:2841118/1‑71 (MQ=255) ttAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCt < 1:4528444/71‑1 (MQ=255) ttAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCt < 1:7770552/71‑1 (MQ=255) aCGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTg > 1:5705385/1‑69 (MQ=255) gCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCTCTGgaga < 1:349367/71‑1 (MQ=255) gCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCACCCATTCGCTGgaga < 1:6280408/71‑1 (MQ=255) aCGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCAt < 1:6265523/50‑1 (MQ=255) cGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAgg > 1:3871670/1‑69 (MQ=255) cTTGATGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCAGGCCATTCCCCCATTCg < 1:5313353/58‑1 (MQ=39) gTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGt < 1:4979237/71‑1 (MQ=255) gTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGt < 1:2396357/71‑1 (MQ=255) aCAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCa > 1:6151480/1‑66 (MQ=255) aCAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCa > 1:2783351/1‑66 (MQ=255) aCAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGAt < 1:3237279/71‑1 (MQ=255) aCAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGAt < 1:6700468/71‑1 (MQ=255) cAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTc < 1:1224310/47‑1 (MQ=255) agagATTGTTTTACCTGCTGGGGAGTGGCAcgccattccgccattc < 1:1231140/46‑1 (MQ=255) gagaTTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGga > 1:378656/1‑51 (MQ=255) gagaTTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTAc < 1:3620276/71‑1 (MQ=255) agaTTGTTTTACCTGCTGGGGAGTGGCACGCCATTc > 1:1317952/1‑36 (MQ=255) agaTTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGc < 1:318645/46‑1 (MQ=255) agaTTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGc < 1:7086343/46‑1 (MQ=255) agaTTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGa > 1:1238355/1‑55 (MQ=255) agaTTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACg > 1:5853160/1‑71 (MQ=255) agaTTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACg > 1:1065879/1‑71 (MQ=255) agaTTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACg > 1:4412035/1‑71 (MQ=255) ttGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCt > 1:2257584/1‑71 (MQ=255) tGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTg > 1:7672307/1‑71 (MQ=255) ttttACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAAcc < 1:1675617/54‑1 (MQ=255) ttttACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTc > 1:883312/1‑71 (MQ=255) ttttACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTc > 1:2206448/1‑71 (MQ=255) ttttACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTc < 1:6207334/71‑1 (MQ=255) ttACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTg > 1:7323207/1‑71 (MQ=255) tACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTg < 1:1161142/70‑1 (MQ=255) tACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTg < 1:756771/70‑1 (MQ=255) tACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTg < 1:5724549/70‑1 (MQ=255) aCCTGCTGGGGAGTGGCACGCTATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTgg < 1:3325704/70‑1 (MQ=255) aCCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTg < 1:5094912/69‑1 (MQ=255) ccTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCa < 1:6528276/71‑1 (MQ=255) ccTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCa < 1:362831/71‑1 (MQ=255) ctgctgGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGt < 1:6613039/64‑1 (MQ=255) ctgctgGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAg > 1:5038890/1‑71 (MQ=255) tgctgGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTc < 1:1725612/64‑1 (MQ=255) ctgGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGa < 1:6149224/71‑1 (MQ=255) tgGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGa < 1:7749815/39‑1 (MQ=255) tgGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGAc > 1:5461095/1‑71 (MQ=255) ggggAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCa < 1:7150982/65‑1 (MQ=255) gggAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGAc < 1:7855066/69‑1 (MQ=255) gggAATGGCGTGCCACTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGAc < 1:110766/69‑1 (MQ=38) gggAATGGCGTGCCACTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGAc < 1:3169231/69‑1 (MQ=38) ggAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGACCTg < 1:1477304/71‑1 (MQ=255) gAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAAcc < 1:789456/40‑1 (MQ=255) aGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGACCTGca > 1:6288287/1‑71 (MQ=255) gTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGACCTGca > 1:6627056/1‑70 (MQ=255) tGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGAt < 1:6836202/44‑1 (MQ=255) tGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGACCTGcaca < 1:1719632/71‑1 (MQ=255) tGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGACCTGcaca < 1:3601144/71‑1 (MQ=255) tGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGACCTGcaca < 1:2169638/71‑1 (MQ=255) gCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTAc > 1:4615087/1‑45 (MQ=255) gCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAggg > 1:7182959/1‑60 (MQ=255) gCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAggg > 1:1924364/1‑60 (MQ=255) gCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAggg > 1:7173559/1‑60 (MQ=255) cACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAg > 1:7877001/1‑37 (MQ=255) cACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGt > 1:5530073/1‑38 (MQ=255) cACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCa > 1:1319621/1‑56 (MQ=255) cACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGACCTGCACACgg < 1:2247559/71‑1 (MQ=255) gCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGACCTGCACACGGAtt < 1:4904224/71‑1 (MQ=255) gCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGACCTGCACACGGAtt > 1:5898155/1‑71 (MQ=255) gCCACTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTg > 1:7486637/1‑50 (MQ=255) gCCACTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTg > 1:2010340/1‑50 (MQ=255) gCCACTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTg > 1:1826812/1‑50 (MQ=255) gCCACTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTg > 1:1724506/1‑50 (MQ=255) cATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGACCTGCACACGGa < 1:292549/67‑1 (MQ=255) cATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGACCTGCACACGGa < 1:3144533/67‑1 (MQ=255) cATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGACCTGCACACGGATtgt < 1:6461994/71‑1 (MQ=255) aTTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGACCTGCACACGGATtgtg > 1:1815192/1‑71 (MQ=255) | TTTTGATCGCAATTAACGCCACGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCAGGGACCTGCACACGGATTGTG > AP009048/4070915‑4071053 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |