| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 4,184,570 | G→T | 7.6% | S90Y (TCT→TAT) | gspC ← | general secretory pathway component, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 4,184,570 | 0 | G | T | 7.6% | 344.8 / 7.8 | 105 | S90Y (TCT→TAT) | gspC | general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base G (39/58); new base T (6/2); total (45/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
CCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCTTTTACCCCAGCATGATTTTCGTTA > AP009048/4184503‑4184638 | ccccTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATaga > 1:4524401/1‑69 (MQ=255) ccTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATagagag < 1:5987243/70‑1 (MQ=255) cTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCTCTCTATAGCGCt > 1:5817377/1‑71 (MQ=255) attaattaAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCTCTCTATAGCGCTTa < 1:7207890/70‑1 (MQ=38) attaattaAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAg > 1:462704/1‑71 (MQ=255) attaattaAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAg > 1:6971277/1‑71 (MQ=255) ttaattaaGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCTCTCTATAGCGCTTaat > 1:5572189/1‑69 (MQ=37) ttaaGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGtt > 1:3293153/1‑71 (MQ=255) taaGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATaga > 1:5927617/1‑57 (MQ=255) taaGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATaga > 1:129124/1‑57 (MQ=255) aaGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCt < 1:3372984/61‑1 (MQ=255) aaGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCt < 1:3476300/61‑1 (MQ=255) aaGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTc > 1:1247002/1‑71 (MQ=255) aaGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTc > 1:4790044/1‑71 (MQ=255) aaGACAAAAGAAGTCTCATCATTCGATGAGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTc > 1:7721733/1‑71 (MQ=255) aaaaGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGAt > 1:2073131/1‑71 (MQ=255) aaaGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGAtt < 1:3706105/71‑1 (MQ=255) aaaGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGAtt < 1:5026325/71‑1 (MQ=255) aagaagTCTCATCATTCGATGTGAGTACAATGCCATTAAGCTCTCTatag > 1:1597508/1‑50 (MQ=255) aagaagTCTCATCATTCGATGTGAGTACAATGCCATTAAGCTCTCTatag > 1:7857770/1‑50 (MQ=255) agaagTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATATAGAGCTTAGGTGTTTCCTGATTaa < 1:1871918/71‑1 (MQ=255) agaagTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTa < 1:7916754/55‑1 (MQ=255) agaagTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTaa < 1:4137830/71‑1 (MQ=255) agaagTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTaa < 1:3022148/71‑1 (MQ=255) agaagTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTaa < 1:2671956/71‑1 (MQ=255) gaagTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAAc < 1:2177471/71‑1 (MQ=255) aagTCTCATGATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACt < 1:5583356/71‑1 (MQ=255) aagTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACt < 1:3134777/71‑1 (MQ=255) aagTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACt < 1:1275916/71‑1 (MQ=255) agTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACt < 1:2316132/70‑1 (MQ=255) gTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAgg < 1:6643235/53‑1 (MQ=255) gTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAgg < 1:3715444/53‑1 (MQ=255) gTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAgg < 1:1315421/53‑1 (MQ=255) gTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAgg < 1:3495242/53‑1 (MQ=255) tctcATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAgg < 1:1717440/52‑1 (MQ=255) tctcATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGcc < 1:2497103/71‑1 (MQ=255) tctcATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGcc < 1:6440155/71‑1 (MQ=255) ctcATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCa > 1:7680516/1‑71 (MQ=255) tcatcaTTCGATGTGAGTACAATGCCATTAAGCTCTCTATAGCGCTTaat > 1:2158074/1‑48 (MQ=21) tcatcaTTCGATGTGAGTACAATGCCATTAAGCTCTCTATAGCGCTTaat > 1:5059164/1‑48 (MQ=21) tcatcaTTCGATGTGAGTACAATGCCATTAAGCTCTCTATAGCGCTTaat > 1:5100275/1‑48 (MQ=21) tcatcaTTCGATGTGAGTACAATGCCATTAAGCTCTCTATAGCGCTTaat > 1:2190831/1‑48 (MQ=21) tcatcaTTCGATGTGAGTACAATGCCATTAAGCGCTATagaga > 1:5120851/1‑43 (MQ=255) atcatTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGttt > 1:7896899/1‑53 (MQ=255) atcatTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGAt > 1:4197705/1‑59 (MQ=255) atcatTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCa > 1:5409187/1‑68 (MQ=255) atcatTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCAc < 1:2660450/69‑1 (MQ=255) catTCGATGTGAGTACAATGCCATTAAGCGCTATGGAGAGCTTAg < 1:7427049/45‑1 (MQ=255) catTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAg < 1:4185169/45‑1 (MQ=255) catTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAg < 1:4116320/45‑1 (MQ=255) catTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAg < 1:5054669/45‑1 (MQ=255) atTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCt > 1:4465862/1‑41 (MQ=255) atTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCt > 1:6532666/1‑41 (MQ=255) atTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCt > 1:6119892/1‑41 (MQ=255) atTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCt > 1:1011996/1‑41 (MQ=255) ttCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGtt < 1:2278766/48‑1 (MQ=255) cGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTc > 1:3961625/1‑48 (MQ=255) aTGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCACggg < 1:3395715/64‑1 (MQ=255) gtgAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCt < 1:111545/46‑1 (MQ=255) gtgAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCt < 1:7215175/46‑1 (MQ=255) gtgAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGAtt < 1:4858926/50‑1 (MQ=255) aGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGtt > 1:4448571/1‑39 (MQ=255) aGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGtt > 1:6550530/1‑39 (MQ=255) aGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGtt > 1:1761004/1‑39 (MQ=255) gTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGa > 1:4830091/1‑44 (MQ=255) gTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCtttt < 1:2445984/71‑1 (MQ=255) tACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGa > 1:230301/1‑43 (MQ=255) tACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCTTTTa < 1:5413207/71‑1 (MQ=255) cAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCt > 1:1904573/1‑39 (MQ=255) cAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCt > 1:1839687/1‑39 (MQ=255) cAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCTTTTAcc < 1:191614/71‑1 (MQ=255) cAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCTTTTAc < 1:7652246/70‑1 (MQ=255) aaTGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCAc < 1:6107562/51‑1 (MQ=255) tGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGAtt > 1:4633808/1‑40 (MQ=255) tGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGAt > 1:2032273/1‑39 (MQ=255) tGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCTTTTAccc > 1:820227/1‑69 (MQ=255) tGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCTTTTACCCCa < 1:7540516/71‑1 (MQ=255) gCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTaa > 1:631734/1‑41 (MQ=255) cATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGc < 1:2328816/43‑1 (MQ=255) aTTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCTTTTACCCCAGCAt > 1:2512653/1‑71 (MQ=255) ttAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCTTTTAc > 1:4114897/1‑62 (MQ=255) ttAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCTTTTACCCCAGCATg < 1:6832301/71‑1 (MQ=255) ttAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCTTTTACCCCAGCATg < 1:3383280/71‑1 (MQ=255) aaGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGc > 1:1763152/1‑39 (MQ=255) aaGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGc > 1:2479446/1‑39 (MQ=255) aGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGc < 1:4946938/38‑1 (MQ=255) aGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGc < 1:2615344/38‑1 (MQ=255) aGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGc < 1:4773447/38‑1 (MQ=255) gcgcTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCTTTTACCCCAGCATg > 1:1590219/1‑67 (MQ=255) gcgcTAAAGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCTTTTACCCCAGCATGAttt > 1:1020781/1‑71 (MQ=255) cgcTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCTTTTACCCCAGCATGAtttt < 1:1073382/71‑1 (MQ=255) gcTATAGAGAGCTTAGGTGCTTCCTGATTAACTGCCACGGGATTTGCATCTTTTACCCCAGCATGATTTTc > 1:5404167/1‑71 (MQ=255) tataGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCTTTTACCACAGCATGATTTTCGt > 1:1268066/1‑71 (MQ=255) ataGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCtttt < 1:716995/51‑1 (MQ=255) taGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCTTTTACCCCAGCATGATTTTCGt < 1:2045282/69‑1 (MQ=255) taGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCTTTTACCCCAGCATGATTTTCGTTa > 1:2267066/1‑71 (MQ=255) gagagCTTAGGTGTTTCCTGTTTAACTGCCACGGGAtt < 1:1062890/38‑1 (MQ=255) gagagCTTAGGTGTTTCCTGATTAACTGCCACGGGAtt < 1:3593625/38‑1 (MQ=255) gagagCTTAGGTGTTTCCTGATTAACTGCCACGGGAtt < 1:3931278/38‑1 (MQ=255) gagagCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCtttt > 1:7788920/1‑48 (MQ=255) | CCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCTGATTAACTGCCACGGGATTTGCATCTTTTACCCCAGCATGATTTTCGTTA > AP009048/4184503‑4184638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |