| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 4,311,972 | A→T | 7.3% | L169Q (CTG→CAG) | alsK ← | D‑allose kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 4,311,972 | 0 | A | T | 7.3% | 146.4 / 2.1 | 53 | L169Q (CTG→CAG) | alsK | D‑allose kinase |
| Reads supporting (aligned to +/- strand): ref base A (25/24); new base T (2/2); total (27/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGC > AP009048/4311902‑4312042 | cgcCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCa < 1:6010360/71‑1 (MQ=255)cgcCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCa < 1:3342307/71‑1 (MQ=255) ccATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAgg < 1:5632194/71‑1 (MQ=255) ttCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACTCGCAGTGTTGGGTCATATCTCCCAgggg > 1:7138180/1‑70 (MQ=255) ccAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGa < 1:184821/70‑1 (MQ=255) ccAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGa > 1:4293080/1‑70 (MQ=255) agCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGa < 1:6280284/71‑1 (MQ=255) gCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGa < 1:7297246/70‑1 (MQ=255) aTTGGTTTCCAGGCACCCAGGATTGCCACACGTGCAGTGTTGGGTCATATCTCCCAGGGGGatat < 1:4253313/65‑1 (MQ=255) aTTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAgg < 1:5939205/58‑1 (MQ=255) aTTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGAcc > 1:5825961/1‑69 (MQ=255) ggTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAg > 1:5219794/1‑54 (MQ=255) ggTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGt > 1:2603593/1‑70 (MQ=255) ggTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGt > 1:7829215/1‑70 (MQ=255) gTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGtt < 1:4838508/70‑1 (MQ=255) tCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGcc > 1:6118296/1‑71 (MQ=255) ccAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGa < 1:279144/54‑1 (MQ=255) cAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGata > 1:4507095/1‑55 (MQ=25) cAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGata > 1:3711675/1‑55 (MQ=25) aGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGc < 1:7683898/71‑1 (MQ=255) ggCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAg > 1:498656/1‑46 (MQ=255) ggCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAg > 1:44297/1‑46 (MQ=255) ggCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAg > 1:1196665/1‑46 (MQ=255) gCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGa > 1:7637531/1‑55 (MQ=255) gCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCca > 1:5636280/1‑71 (MQ=255) cccAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGt < 1:1905303/58‑1 (MQ=255) cccAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGt < 1:2556360/58‑1 (MQ=255) ccAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGatat < 1:3884894/49‑1 (MQ=255) ccAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGatat < 1:5362441/49‑1 (MQ=255) ccAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGatat < 1:7029045/49‑1 (MQ=255) cAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAgg < 1:2029082/41‑1 (MQ=255) cAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCg < 1:2491336/71‑1 (MQ=255) cAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCg < 1:3964394/71‑1 (MQ=255) aGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCgt < 1:3027843/71‑1 (MQ=255) aGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCgt < 1:5061556/71‑1 (MQ=255) ggATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCgtg < 1:1288495/71‑1 (MQ=255) gATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCgtgt < 1:325149/71‑1 (MQ=255) gATTGACACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACCCCgtgt > 1:2231038/1‑71 (MQ=255) cccgcgCAGTGTTGTGTCATATCTCCCTGGGTGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGt < 1:1675707/69‑1 (MQ=255) tgtTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGgcgc < 1:4669530/71‑1 (MQ=255) gtTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCa > 1:1897395/1‑71 (MQ=255) tCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGc < 1:6773618/49‑1 (MQ=255) atatCTCCCAGGGGGATATGCCCCAGTTCGCCTGCCCCACCGTGTGCACCCGTCCACGGCGCACCGTTCa > 1:2134283/1‑70 (MQ=255) atatCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCa < 1:1622652/70‑1 (MQ=255) atatCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCAt > 1:7702335/1‑71 (MQ=255) atCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCgtg < 1:1005745/42‑1 (MQ=18) tctcCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCAt > 1:7939607/1‑68 (MQ=255) tctcCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCAt > 1:7907842/1‑68 (MQ=255) tctcCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCAt > 1:5975534/1‑68 (MQ=255) ctcCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCAc > 1:3162310/1‑60 (MQ=255) cccAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCAc > 1:4009314/1‑43 (MQ=255) cccAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCAc > 1:4426371/1‑43 (MQ=255) cccAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCAc > 1:6215562/1‑43 (MQ=255) cccAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCAt > 1:7878764/1‑65 (MQ=255) ccAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACt > 1:499503/1‑71 (MQ=255) aGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGc > 1:7601632/1‑71 (MQ=255) aGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGc > 1:6489896/1‑71 (MQ=255) | CGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGC > AP009048/4311902‑4312042 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |