| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 4,609,912 | T→G | 6.5% | E144D (GAA→GAC) | fhuF ← | ferric iron reductase involved in ferric hydroximate transport |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 4,609,912 | 0 | T | G | 6.5% | 234.4 / 11.4 | 92 | E144D (GAA→GAC) | fhuF | ferric iron reductase involved in ferric hydroximate transport |
| Reads supporting (aligned to +/- strand): ref base T (43/43); new base G (2/4); total (45/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
GAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCGGTTTCGTGAAACTCAGCATGGAAATGTTCC > AP009048/4609845‑4609978 | gAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTca < 1:1299682/71‑1 (MQ=255)gAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTca > 1:3718481/1‑71 (MQ=255)gAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTca < 1:3831616/71‑1 (MQ=255) aCCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTcaca > 1:5384058/1‑71 (MQ=255) ccAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTcacac < 1:4525907/71‑1 (MQ=255) aGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTcacaca > 1:6466774/1‑70 (MQ=255) gcgcCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTcacacac > 1:3148519/1‑70 (MQ=255) cgcCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTcacacaca > 1:3563841/1‑70 (MQ=255) gcCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATc < 1:1208238/71‑1 (MQ=255) gcCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATc < 1:3184143/71‑1 (MQ=255) ccTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTca < 1:6150064/62‑1 (MQ=255) ccTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTca < 1:5355938/62‑1 (MQ=255) cTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCt < 1:3724004/58‑1 (MQ=255) cTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCt < 1:1584719/58‑1 (MQ=255) cTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCt < 1:5011507/58‑1 (MQ=255) cTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCt < 1:750908/58‑1 (MQ=255) tGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCt < 1:1755133/57‑1 (MQ=255) tGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGAc > 1:2596594/1‑71 (MQ=255) ggCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTcaca > 1:2518684/1‑61 (MQ=255) gCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGAccc < 1:7100851/71‑1 (MQ=255) tGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCtt < 1:7460721/54‑1 (MQ=255) tGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCtt < 1:2341359/54‑1 (MQ=255) tGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCtt < 1:4246624/54‑1 (MQ=255) tGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTc < 1:28236/55‑1 (MQ=255) gATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCa > 1:5900560/1‑69 (MQ=255) ttAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATc < 1:4491983/61‑1 (MQ=255) ttAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATc < 1:1976580/61‑1 (MQ=255) ttAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATc < 1:4108040/61‑1 (MQ=255) aaCGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTca > 1:5345596/1‑51 (MQ=255) aaCGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGaaa > 1:3966561/1‑69 (MQ=255) aaCGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAAc < 1:4818912/70‑1 (MQ=255) aCGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAg > 1:3769507/1‑71 (MQ=255) gTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCg < 1:3172289/57‑1 (MQ=255) gTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCg < 1:3729401/57‑1 (MQ=255) gTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAgg < 1:503090/70‑1 (MQ=255) gTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGc < 1:6373998/71‑1 (MQ=255) gTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGc < 1:1216790/71‑1 (MQ=255) gTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGc < 1:4664274/71‑1 (MQ=255) tttCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAgg < 1:1963853/69‑1 (MQ=255) ttCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCtt > 1:7069385/1‑44 (MQ=255) ttCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAgg > 1:1628135/1‑68 (MQ=255) ttCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAgg > 1:2917468/1‑68 (MQ=255) ttCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCAAGAAACAgg > 1:3981574/1‑68 (MQ=255) tCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGAc > 1:7396678/1‑71 (MQ=255) ccATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACAt > 1:3170592/1‑51 (MQ=255) ccATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATc < 1:6896804/52‑1 (MQ=255) cATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTcac > 1:5337382/1‑44 (MQ=255) cATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGAcg < 1:4446002/70‑1 (MQ=255) cGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAgg > 1:4419173/1‑61 (MQ=255) cGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAgg > 1:1597092/1‑61 (MQ=255) cGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTcc > 1:2899865/1‑71 (MQ=255) agATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTc > 1:7146194/2‑70 (MQ=255) gATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGc < 1:5433765/61‑1 (MQ=255) gATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTcc < 1:7932280/70‑1 (MQ=255) aTGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGaaa < 1:2517375/55‑1 (MQ=255) tgctgcGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGa > 1:6805122/1‑61 (MQ=255) tgctgcGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGa > 1:6185399/1‑61 (MQ=255) tgctgcGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGa > 1:5004355/1‑61 (MQ=255) ctgcGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCGGttt > 1:4518919/1‑71 (MQ=255) ctgcGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCGGtt < 1:5814660/70‑1 (MQ=255) ctgcGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCGGtt < 1:2390063/70‑1 (MQ=255) gcgAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCGGTTTCGTGa < 1:3534692/71‑1 (MQ=255) aaTGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAAc < 1:171017/45‑1 (MQ=255) aaTGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAAc < 1:271125/45‑1 (MQ=255) tgtgGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCGGTTTCGTGAAACt > 1:1319513/1‑70 (MQ=255) tggtgTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGc < 1:7389618/45‑1 (MQ=255) tggtgTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGc < 1:7346643/45‑1 (MQ=255) tggtgTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGa > 1:1125342/1‑47 (MQ=255) tggtgTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCGGTTTCGTGAAACTCAg > 1:2179439/1‑71 (MQ=255) gtgtTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGt < 1:4844943/50‑1 (MQ=255) gtgtTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGt < 1:6915520/50‑1 (MQ=255) gtgtTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCGGTTTCGTGAAACTCAGc < 1:5696097/70‑1 (MQ=255) gtTGCGTTTTTATCTTCACACACATCGACCCAGaaa < 1:4679838/36‑1 (MQ=255) gtTGCGTTTTTATCTTCACACACATCGACCCAGaaa < 1:6177513/36‑1 (MQ=255) ttGCGTTTTTATCTTCACACACATCGACCCAGAAACAgg > 1:7922548/1‑39 (MQ=255) ttGCGTTTTTATCTTCACACACATCGACCCAGAAACAgg > 1:7711778/1‑39 (MQ=255) ttGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGa > 1:3562038/1‑42 (MQ=255) ttGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGa > 1:429632/1‑42 (MQ=255) ttGCGTTTTTATCGTCACACACATCGACCCAGAAAc < 1:5171989/36‑1 (MQ=255) ttGCGTTTTTATCGTCACACACATCGACCCAGAAAc < 1:4666019/36‑1 (MQ=255) ttGCGTTTTTATCGTCACACACATCGACCCAGAAAc < 1:5259782/36‑1 (MQ=255) ttGCGTTTTTATCGTCACACACATCGACCCAGAAAc < 1:3962464/36‑1 (MQ=255) tccGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGt > 1:6756205/3‑46 (MQ=255) gCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTc < 1:3930536/46‑1 (MQ=255) gCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTc < 1:2823272/46‑1 (MQ=255) gCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCGGTTTCGTGAAACt > 1:3860695/1‑61 (MQ=255) gCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCGGTTTCGTGAAACTCAGCATgg > 1:124750/1‑69 (MQ=255) cGTTTTTATCTTCACACTCATCGACCCAGAAACAGGCGACGCGTCCGGTTTCGTGAAACTCAGCATGGaa < 1:2754343/70‑1 (MQ=255) cGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTc < 1:7060184/45‑1 (MQ=255) gTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCg > 1:7733266/1‑46 (MQ=255) gTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCGGTTTCGTGAAACTCAGCATGGAAAt > 1:3535062/1‑71 (MQ=255) tttttATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCGGTTTCGTGAAACTCAGCATGGAAATg > 1:467168/1‑71 (MQ=255) tttttATCGTCACACACATCGACCCAGAAACAGGCGGCACGGCCGGTTTCGTGAAACTCAGCAt > 1:4313486/1‑64 (MQ=255) tttttATCGTCACACACATCGACCCAGAAACAGGCGGCACGGCCGGTTTCGTGAAACTCAGCAt > 1:5798674/1‑64 (MQ=255) ttttATCTTCACACACATCGACCCAGAAACAGGCg > 1:6661058/1‑35 (MQ=255) tttATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCGGTTTCGTGAAACTCAGCATGGAAATGtt > 1:1915113/1‑71 (MQ=255) tttATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCGGTTTCGTGAAACTCAGCATGGAAATGtt > 1:4894513/1‑71 (MQ=255) tttATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCGGTTTCGTGAAACTCAGCATGGAAATGt < 1:1308101/70‑1 (MQ=255) ttATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCGGtt < 1:3448621/45‑1 (MQ=255) ttATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCGGTTTCGTGAAACTCAGCATGGAAATGTTc > 1:6510551/1‑71 (MQ=255) | GAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTGGTGTTGCGTTTTTATCTTCACACACATCGACCCAGAAACAGGCGACGCGTCCGGTTTCGTGAAACTCAGCATGGAAATGTTCC > AP009048/4609845‑4609978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |