| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 236,101 | A→C | 79.8% | D12A (GAT→GCT) | dnaQ → | DNA polymerase III epsilon subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 236,101 | 0 | A | C | 79.8% | 1885.2 / 278.4 | 681 | D12A (GAT→GCT) | dnaQ | DNA polymerase III epsilon subunit |
| Reads supporting (aligned to +/- strand): ref base A (70/68); new base C (293/249); total (364/317) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.20e-01 | |||||||||||
Only 100 of 689 total aligned reads displayed.
ACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCC > AP009048/236032‑236171 | aCTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGa > 1:2622251/1‑70 (MQ=255) gcgtacaactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTAc > 1:2255740‑M2/11‑71 (MQ=255) gtacaactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCg > 1:6949643‑M2/9‑71 (MQ=255) gtacaactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCg > 1:6164143‑M2/9‑71 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:3007664‑M2/6‑71 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:4753763‑M2/6‑71 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:5468908‑M2/6‑71 (MQ=255) cGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAacc < 1:6610053/71‑1 (MQ=255) ctCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTa < 1:2369407‑M2/60‑1 (MQ=255) ctCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAacca > 1:1007987‑M2/3‑71 (MQ=255) ctCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAacca > 1:6059205‑M2/3‑71 (MQ=255) tCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTAccgca < 1:94161‑M2/65‑3 (MQ=255) cAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAaccacc < 1:4450826/71‑1 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg < 1:4168394/71‑1 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg > 1:3562732/1‑71 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg > 1:2724213/1‑71 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAaccac > 1:5850072/1‑69 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTCCAATTACACGCCAGATCGTTCTCGCTACCGCAaccacc < 1:5887597/70‑1 (MQ=255) aGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg < 1:5155581/71‑1 (MQ=255) gTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCgca < 1:6837388/62‑3 (MQ=255) gTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg > 1:7107839/1‑70 (MQ=255) tGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTAt > 1:7019119/1‑70 (MQ=255) tGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTAt > 1:1098055/1‑70 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGaa > 1:1927370/1‑71 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGaa > 1:4283961/1‑71 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa < 1:7627486/70‑1 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa < 1:146536/70‑1 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa < 1:4910447/70‑1 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGaa > 1:6453366/1‑71 (MQ=255) aTAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAac < 1:6685854/57‑1 (MQ=255) aTAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAac < 1:3984915/57‑1 (MQ=255) tAAATTACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa < 1:6086221/71‑1 (MQ=255) tAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa < 1:2061799/71‑1 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCg < 1:4982398/51‑1 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAacca > 1:3867051/1‑57 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:6262299/1‑68 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCGGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:5228035/1‑67 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTAt > 1:640929/1‑63 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:2437997/1‑67 (MQ=255) aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg < 1:2925599/59‑1 (MQ=255) aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAt < 1:2330768/71‑1 (MQ=255) gACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccacc < 1:850088/55‑1 (MQ=255) aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCgca < 1:1612060/48‑3 (MQ=255) cGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCg > 1:4531475/1‑44 (MQ=255) gCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgc > 1:3435216/1‑71 (MQ=255) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgcg > 1:7891808/1‑71 (MQ=255) aTGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTa > 1:4702941/1‑52 (MQ=255) aTGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCa > 1:1984573/1‑71 (MQ=255) tGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCAc < 1:1756076/71‑1 (MQ=255) gAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa > 1:4095601/1‑57 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:1147802/1‑40 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:5042650/1‑40 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAcc < 1:1560773/55‑1 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTa < 1:7275392/71‑1 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTa < 1:1806257/71‑1 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:203284/71‑1 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:7060756/71‑1 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:5308080/71‑1 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:2151342/1‑36 (MQ=38) cTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTa < 1:4660062/45‑1 (MQ=38) tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg > 1:4600539/1‑41 (MQ=255) tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATg < 1:393394/46‑1 (MQ=39) tGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTAt < 1:6224793/67‑1 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg > 1:3093526/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCTCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc < 1:274497/71‑1 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg > 1:899808/1‑70 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:5773138/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTa > 1:691255/1‑64 (MQ=255) aaaTTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATg > 1:5664377/2‑44 (MQ=38) aTTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:1253268/1‑45 (MQ=38) aTTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCca < 1:745732/71‑1 (MQ=255) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt < 1:3169465/62‑1 (MQ=255) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg > 1:1317819/1‑63 (MQ=255) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg > 1:6742845/1‑63 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa < 1:5103860/40‑1 (MQ=38) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:556902/1‑42 (MQ=38) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:7207730/1‑69 (MQ=255) cacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa > 1:6004346/1‑43 (MQ=38) cacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGTTTGGTGCGCACTATGAAGGCCACaa < 1:4365580/70‑1 (MQ=255) cacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTa < 1:1838047/58‑1 (MQ=255) acGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgcg > 1:3714299/1‑52 (MQ=255) gCCAGATCGTTTTCGCTACCGCAACCACCGGTATGAACCAGAtt < 1:4217774/44‑1 (MQ=25) aGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAATATCAtt < 1:3354295/71‑1 (MQ=255) aGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt < 1:7567058/71‑1 (MQ=255) gATCGTTCTCGCTACCGCAACCACCTGTATGAACCAGATTGGTGCGCACTATg < 1:3231219/53‑1 (MQ=255) gATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTTCGCACTATGaa > 1:3768270/1‑55 (MQ=255) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCa < 1:7765007/35‑1 (MQ=37) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:2976309/52‑1 (MQ=255) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga < 1:2846552/71‑1 (MQ=255) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:2528684/1‑68 (MQ=255) cGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:3636719/1‑67 (MQ=255) gTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAt < 1:3527767/71‑1 (MQ=255) gTTATCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTgaga < 1:2671277/70‑1 (MQ=255) ttCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTg < 1:635297/66‑1 (MQ=255) ttCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAtt > 1:228117/1‑71 (MQ=255) ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTgg > 1:3467980/1‑71 (MQ=255) ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTg < 1:7331915/70‑1 (MQ=255) ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTg < 1:1894453/70‑1 (MQ=255) tcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAt < 1:1689959/61‑1 (MQ=255) tcGATACCGAAACCACCGGTATGAACCAGATTGGTgcg < 1:5376142/38‑1 (MQ=255) | ACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCC > AP009048/236032‑236171 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |