| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 281,467 | T→C | 6.4% | intergenic (‑260/‑14) | yagA ← / → yagE | predicted DNA‑binding transcriptional regulator/predicted lyase/synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 281,467 | 0 | T | C | 6.4% | 262.6 / 6.5 | 93 | intergenic (‑260/‑14) | yagA/yagE | predicted DNA‑binding transcriptional regulator/predicted lyase/synthase |
| Reads supporting (aligned to +/- strand): ref base T (43/44); new base C (3/3); total (46/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 102 total aligned reads displayed.
CCCTTCCGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCC > AP009048/281400‑281537 | cccttccGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCtat < 1:7561986/68‑1 (MQ=255)cccttccGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCtat < 1:1233771/68‑1 (MQ=255) ccGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAg > 1:490412/1‑70 (MQ=255) ccGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAg > 1:3757659/1‑70 (MQ=255) ccGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAg > 1:6255713/1‑70 (MQ=255) ccGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAg > 1:6126176/1‑70 (MQ=255) cGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATATTATAGaa < 1:6575941/71‑1 (MQ=255) gATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCtata < 1:5249897/62‑1 (MQ=255) gATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCtata < 1:2839651/62‑1 (MQ=255) gATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGaa > 1:6917864/1‑70 (MQ=255) gATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGCTCtata < 1:1024750/62‑1 (MQ=255) tttACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACa > 1:7639474/1‑70 (MQ=255) tttACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTACATTATAGaa > 1:6536296/1‑68 (MQ=255) tttACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTACATTATAGaa > 1:2430532/1‑68 (MQ=255) tttACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTACATTATAGaa > 1:2424635/1‑68 (MQ=255) ttACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATaa > 1:730736/1‑60 (MQ=255) aCCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACaaaa < 1:5751288/70‑1 (MQ=255) ctctCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGt > 1:6079022/1‑59 (MQ=255) ctctCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAAt < 1:4607699/69‑1 (MQ=255) ctctCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGa < 1:5333116/71‑1 (MQ=255) ctcCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTa > 1:432553/1‑58 (MQ=255) cTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGAtt < 1:4509334/68‑1 (MQ=255) gTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGc < 1:5005513/69‑1 (MQ=255) cGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCATCAAGgag < 1:1087739/69‑1 (MQ=255) cGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGgaga < 1:7039784/70‑1 (MQ=255) cGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGgag < 1:4181812/69‑1 (MQ=255) gTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGAtc < 1:4137231/71‑1 (MQ=255) tCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATg < 1:6118683/42‑1 (MQ=255) tCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAg < 1:4265104/60‑1 (MQ=255) tCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAg < 1:4119151/60‑1 (MQ=255) tCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGgag < 1:2692638/67‑1 (MQ=255) tCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGgag < 1:4179382/67‑1 (MQ=255) tCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGAtct > 1:6153583/1‑71 (MQ=255) tCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGAtct < 1:1064177/71‑1 (MQ=255) tCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGAtc > 1:6273007/1‑70 (MQ=255) aTAGTATGATCGTTAAATAAACGAACTCTGTTCTATAATGTAGAACAAAATGATTCAGCa < 1:4873981/60‑1 (MQ=255) aTAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCa < 1:609944/60‑1 (MQ=255) aTAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCa > 1:672493/1‑60 (MQ=255) aTAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCa < 1:453463/60‑1 (MQ=255) aTAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCa > 1:543205/1‑71 (MQ=255) aTAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCa > 1:6965878/1‑71 (MQ=255) aTAGTATGATCGATAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGAtctc > 1:2609032/1‑70 (MQ=255) aTAGTAGGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCa > 1:5341/1‑71 (MQ=255) tAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGt < 1:2848051/40‑1 (MQ=255) tAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTa < 1:5558990/41‑1 (MQ=255) gTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTc > 1:5283960/1‑53 (MQ=255) gTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGc > 1:4381938/1‑71 (MQ=255) gTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGc > 1:3071101/1‑71 (MQ=255) tATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGcc > 1:2538096/1‑71 (MQ=255) tATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGc < 1:6776212/70‑1 (MQ=255) aTGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAAt < 1:3023817/46‑1 (MQ=255) tGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCg > 1:5475826/1‑70 (MQ=255) aTCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGAtt > 1:5987454/1‑47 (MQ=255) aTCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCa < 1:5862311/70‑1 (MQ=255) cGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAAt > 1:5366599/1‑41 (MQ=255) cGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTc > 1:3251992/1‑71 (MQ=255) cGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTc > 1:3372525/1‑71 (MQ=255) cGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTc > 1:1950095/1‑71 (MQ=255) gTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAgg < 1:5223138/52‑1 (MQ=255) ttAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATg < 1:6492225/40‑1 (MQ=255) taaataaaCGAACGCTGTTCTATAATGTAGAACAAAAt < 1:6641321/38‑1 (MQ=255) aaataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCcgcg > 1:2345891/1‑71 (MQ=255) aaataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCcgc < 1:3693098/70‑1 (MQ=255) aataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCgt > 1:5974070/1‑71 (MQ=255) ataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCa < 1:445809/41‑1 (MQ=255) ataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCa < 1:7811676/41‑1 (MQ=255) ataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGAtctc < 1:1868853/54‑1 (MQ=255) ataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGAtctc < 1:4483953/54‑1 (MQ=255) ataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGAtctc < 1:6313564/54‑1 (MQ=255) ataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGAtctc < 1:7882778/54‑1 (MQ=255) ataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGAtctc < 1:4152298/54‑1 (MQ=255) aaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCaa > 1:2179177/1‑43 (MQ=255) aaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAg > 1:452925/1‑44 (MQ=255) aaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGgag > 1:4163657/1‑47 (MQ=255) aaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGgag > 1:5851716/1‑47 (MQ=255) aacgaacgCTGTTCTATAATGTAGAACAAAATGATTCAGCaa < 1:2981770/42‑1 (MQ=255) aacgaacgCTGTTCTATAATGTAGAACAAAATGATTCAGCaa < 1:1401791/42‑1 (MQ=255) aacgaacgCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCgttgtt < 1:2661578/71‑1 (MQ=255) gaacgCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCAc > 1:2511477/1‑71 (MQ=255) acgCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCgttgt > 1:501378/1‑65 (MQ=255) ttttGTTCTACATTATAGAACAGAATGATTCAGCAAGGAGATCTCATGCCGCAGTCcgcg < 1:6542623/57‑1 (MQ=25) ttttGTTCTACATTATAGAACAGAATGATTCAGCAAGGAGATCTCATGCCGCAGTCcgcg < 1:1620353/57‑1 (MQ=25) cgCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACgg > 1:7927700/1‑70 (MQ=255) tttGTTCTACATTATAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTcc < 1:4026281/54‑1 (MQ=37) gCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGAt > 1:6179362/1‑41 (MQ=255) gCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGAt > 1:1861016/1‑41 (MQ=255) gCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGAt > 1:4977244/1‑41 (MQ=255) gCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGAt > 1:7684649/1‑41 (MQ=255) gCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGa < 1:2400836/71‑1 (MQ=255) tGTTCTATAATGTAGAACAAAATGATTCAGCAAGga < 1:11211/36‑1 (MQ=255) tGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCgttgtt < 1:4116765/62‑1 (MQ=255) tGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAAt > 1:727059/1‑71 (MQ=255) ttCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGc < 1:3221803/47‑1 (MQ=255) ttCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATc < 1:7899816/70‑1 (MQ=255) tCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCCCGGGAATCAt > 1:1087706/1‑71 (MQ=255) cTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCAtt > 1:7548570/1‑71 (MQ=255) tataATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCcgcg < 1:6784368/52‑1 (MQ=255) ataATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAAt > 1:7749231/1‑65 (MQ=255) taATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGaa > 1:4460215/1‑63 (MQ=255) taATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTccc > 1:1393273/1‑71 (MQ=255) | CCCTTCCGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCC > AP009048/281400‑281537 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |