Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AP009048 547,694 A→G 100% pseudogene (114/261 nt)
pseudogene (114/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP009048547,6940AG100.0% 314.2 / NA 90pseudogene (114/261 nt)
pseudogene (114/1259 nt)
ylbE
ylbE
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (47/43);  total (47/43)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGG  >  AP009048/547631‑547761
                                                               |                                                                   
tcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa                                                              >  1:5924029/1‑71 (MQ=255)
    gcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACt                                                          >  1:7170142/1‑71 (MQ=255)
      tCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGc                                                        >  1:2536725/1‑71 (MQ=255)
        gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa                                                              >  1:3536714/1‑63 (MQ=255)
        gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa                                                              >  1:7862048/1‑63 (MQ=255)
        gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa                                                              >  1:2749219/1‑63 (MQ=255)
         tCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTc                                                     <  1:3869207/71‑1 (MQ=255)
             cacTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGc                                                 <  1:154908/71‑1 (MQ=255)
               ctggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa                                                              <  1:2327204/56‑1 (MQ=255)
               ctggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa                                                              <  1:6972456/56‑1 (MQ=255)
                tggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAac                                                             <  1:589814/56‑1 (MQ=255)
                 ggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAac                                                             <  1:3570915/55‑1 (MQ=255)
                    tggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccg                                          >  1:2819293/1‑71 (MQ=255)
                     ggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgc                                         >  1:4948252/1‑71 (MQ=255)
                      gATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc                                        <  1:3680676/71‑1 (MQ=255)
                      gATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc                                        <  1:7478964/71‑1 (MQ=255)
                      gATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgc                                         >  1:3909403/1‑70 (MQ=255)
                        tgtgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa                                      <  1:3081394/71‑1 (MQ=255)
                          tgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATg                                    >  1:5832198/1‑71 (MQ=255)
                           gCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgc                                   >  1:6973459/1‑71 (MQ=255)
                            cAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgc                                            >  1:4885387/1‑61 (MQ=255)
                            cAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgcg                                  >  1:1857700/1‑71 (MQ=255)
                             aaCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgcc                                           <  1:7703263/61‑1 (MQ=255)
                             aaCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgcgc                                 >  1:1599334/1‑71 (MQ=255)
                              aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa                                                              >  1:7382177/1‑41 (MQ=255)
                              aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa                                                              >  1:1882860/1‑41 (MQ=255)
                              aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCa                                                                 <  1:4284346/38‑1 (MQ=255)
                              aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCt                                <  1:3315813/71‑1 (MQ=255)
                               ccGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCggg                                             >  1:1603525/1‑57 (MQ=255)
                               ccGGCTTCTTAACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgc                                         >  1:2873995/1‑61 (MQ=255)
                                  gCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTgg                              >  1:868031/1‑69 (MQ=255)
                                   cttcttCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg                           <  1:5023774/71‑1 (MQ=255)
                                    ttcttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgc                                   <  1:549107/62‑1 (MQ=255)
                                      cttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAgg                          <  1:187725/69‑1 (MQ=255)
                                      cttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAgg                          <  1:5530445/69‑1 (MQ=255)
                                      cttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga                         <  1:5407191/70‑1 (MQ=255)
                                      cttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga                         <  1:2422077/70‑1 (MQ=255)
                                       ttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa                                      >  1:5404250/1‑56 (MQ=255)
                                       ttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa                                      >  1:2272267/1‑56 (MQ=255)
                                       ttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCa                                       >  1:3973560/1‑55 (MQ=255)
                                        tcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCa                                                    >  1:3753697/1‑41 (MQ=255)
                                        tcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGc                             >  1:6305907/1‑64 (MQ=255)
                                         cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgaga                       >  1:3236096/1‑69 (MQ=255)
                                         cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGAt                      <  1:2427925/70‑1 (MQ=255)
                                          aCTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATg                     <  1:5950369/70‑1 (MQ=255)
                                           cTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCa                                                    >  1:7758258/1‑38 (MQ=255)
                                           cTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCAGCCAATGCGCTGGCAGGAGATGAc                   >  1:5960138/1‑71 (MQ=255)
                                            ttATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCg                                               <  1:3064278/42‑1 (MQ=255)
                                            ttATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCg                                               <  1:1629657/42‑1 (MQ=255)
                                            ttATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGAcc                  >  1:3236425/1‑71 (MQ=255)
                                              aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACg                                                  <  1:7809005/37‑1 (MQ=255)
                                              aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACg                                                  >  1:3412403/1‑37 (MQ=255)
                                              aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACg                                                  <  1:6016398/37‑1 (MQ=255)
                                              aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCg                                               >  1:3189977/1‑40 (MQ=255)
                                              aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCg                                               >  1:720337/1‑40 (MQ=255)
                                               tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgaga                       >  1:3494838/1‑63 (MQ=255)
                                               tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgaga                       >  1:2829397/1‑63 (MQ=255)
                                                 aGCGAACTAAACGAGGGCAAAACACTGCTTCACGc                                                 >  1:3564832/1‑35 (MQ=255)
                                                  gCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCt                                <  1:1158993/51‑1 (MQ=255)
                                                  gCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCt                                <  1:6037993/51‑1 (MQ=255)
                                                  gCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCt                                <  1:2377863/51‑1 (MQ=255)
                                                  gCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAccc            <  1:2998884/71‑1 (MQ=255)
                                                   cGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa                                      >  1:4926141/1‑44 (MQ=255)
                                                   cGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa                                      >  1:5552281/1‑44 (MQ=255)
                                                   cGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg                           <  1:1686518/55‑1 (MQ=255)
                                                    gAACTAAACGAGGGCAAAACACTGCTTCACGCCGTGCCGCCaa                                      >  1:250564/1‑43 (MQ=38)
                                                    gAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg                 >  1:4465780/1‑64 (MQ=255)
                                                    gAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg                 >  1:4559208/1‑64 (MQ=255)
                                                     aaCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga                         >  1:6924627/1‑55 (MQ=255)
                                                     aaCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga                         >  1:4652969/1‑55 (MQ=255)
                                                     aaCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga                         >  1:1862631/1‑55 (MQ=255)
                                                      aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa        <  1:6166055/71‑1 (MQ=255)
                                                      aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa        <  1:2660775/71‑1 (MQ=255)
                                                      aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa        <  1:714744/71‑1 (MQ=255)
                                                       cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATg         >  1:4783028/1‑69 (MQ=255)
                                                       cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGaa       <  1:4875446/71‑1 (MQ=255)
                                                        tAAACGAGGGCAAAACACTGCTTCACGCCGGgccgc                                         <  1:6454422/36‑1 (MQ=255)
                                                        tAAACGAGGGCAAAACACTGCTTCACGCCGGgccgc                                         <  1:6463705/36‑1 (MQ=255)
                                                        tAAACGAGGGCAAAACACTGCTTCACGCCGGgccgc                                         <  1:5538353/36‑1 (MQ=255)
                                                        tAAACGAGGGCAAAACACTGCTTCACGCCGGgccgc                                         <  1:1646531/36‑1 (MQ=255)
                                                        tAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa        >  1:1919307/1‑69 (MQ=255)
                                                          aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATg         <  1:7480869/66‑1 (MQ=255)
                                                          aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGaaa      <  1:3012084/69‑1 (MQ=255)
                                                          aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgg    <  1:2624597/71‑1 (MQ=255)
                                                          aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAg     >  1:7463557/1‑70 (MQ=255)
                                                          aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAg     <  1:1851724/70‑1 (MQ=255)
                                                           aCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAggg   <  1:7035797/71‑1 (MQ=255)
                                                           aCGAGGGCAAAACACTGCTTCACACCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAggg   <  1:6031263/71‑1 (MQ=255)
                                                            cGAGGGCAAAACACTGGTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgggg  >  1:6314987/1‑71 (MQ=255)
                                                            cGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgcgc                                 >  1:6550700/1‑40 (MQ=255)
                                                               |                                                                   
TCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGG  >  AP009048/547631‑547761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: